Zetasizer Nano accessories guide
Page 6-1
6
Advanced
protein features
Introduction
This chapter gives an overview of the
Advanced
protein features
option in the
Zetasizer software.
With the Zetasizer Nano ZSP it is now possible to achieve the best possible
measurement of
protein mobilities
. This is achieved with the combination of
the following features:
A system with sufficient sensitivity to measure the low count rates and low
electrophoretic mobilities associated with protein samples.
A measurement technique that minimises the risk of protein aggregation and
minimise the amount of material required. This is the
Diffusion barrier
measurement technique; a description of this follows.
A measurement process that reduces the risk of aggregation but also capable of
identifying any aggregates that are formed and assesses the quality of the
measurement.
Measurement process
The protein mobility measurement combines
size
and
zeta
potential
measurements to check that no protein aggregates are forming during the
measurement.
The mobility measurements will be performed in
groups
of sub-runs. This is to
allow for periods of cooling to be applied at stages within the measurement and so
reduce any chances of aggregation happening through over-heating of the sample.
To further reduce any risk of aggregation, an automated measurement
optimization will check and reduce the voltage required. This is done by
monitoring the sample conductivity.
