Chapter 4
Flow-mode option
Page 4-6
MAN 0487
Traces
The traces on the plot display the measured parameters with
respect to the volume passed through the flow-mode system
arrangement. Use the cursor to select each trace; the trace
selected will be displayed in the plot legend and the displayed
Trace
tab.
The traces shown can be chosen by right-clicking on the y-
axes or by using the
Trace
tab.
Generally the
Z-average mean
values are shown as dots on
the plot, whilst the
Intensity
is shown as a continuous line.
Peaks
The
peaks
detected by the analysis are indicated by the
blue
vertical bars
on the plot. The peaks displayed are determined
by inspecting the Z-average mean data points.
When a peak is selected, by either clicking on the bar or
selecting the peak check box, the
Peak
tab will be displayed.
This shows the peak details (see below). Note that the
selected peak will be highlighted deep blue.
By clicking and dragging, it is possible to select a region of the
chromatogram, which is highlighted in purple. By right clicking
the mouse, it is then possible to perform a DLS analysis of this
region. This calculates and intensity-weighted average Z-aver-
age that is calculated in the box in the bottom right of the dis-
play.
Note that when the user clicks
OK
, although the data is saved,
it is not displayed in the reports and must be accessed by
returning to the
Edit results
dialogue.
Viewing tools
These enable different plot views to be shown. The tools can
be selected and used in two ways:
•
Select directly from the drop-down menu and perform the
action.
•
With the drop-down menu showing the Peak/trace high-
lighter, hold the appropriate control key down to perform the
action.
The actions available are:
Peak / Trace highlighter (cursor)
When the cursor is moved over the traces, peaks and axes of
the plot, this will change to a hand, allowing the feature to be
selected. When a trace or peak is selected the respective tab
will be displayed.
Zoom
(Alt)
Use to place a “marquee” around a specific part of the plot
and enlarge. The axes will automatically adjust to match the
enlarged plot.