1. Rotate the revolving nosepiece so that the 10X
objective comes above the specimen.
Here, we use the 10X objective because it makes
focusing easiest.
2. While looking at the microscope from the side, rotate
the coarse focus adjustment knob to approach the
specimen until it is as close as possible to the
objective.
3. While observing the specimen through the eyepiece
lenses, lower the stage by slowly rotating the coarse
focus adjusment knob and, when coarse focusing is
obtained, adjust to precise focus by rotating the fine
focus adjustment knob ‚.
The following table shows the distance between
the objective and specimen (“A” in Fig. 18) when
precise focus is obtained.
Fig. 18
29.0
6.30
0.6
mm
Mm
mm
1
2
1
1
2
0.16
mm
100x
A
