MagCore EDA Operation Manual Download Page 6

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Model

EDA

System Method 

Cellulose Coated Magnetic Beads

System Components

1.  Pipetting Unit: dispensing, transferring, 2 X-Y axis model 

2.  Electric Control: PLC model and Arm-based main board embedded in

3.  UV Light: power 8W, life duration 11,000hr

4.  Contamination Prevention: HEPA filtration, Pipette tips with filter

5.  Heating Block: RT-95

o

C

6.  Nucleic Acid Concentration Detection Source: D2 lamp

7.  Detection Wavelength: 230nm, 260nm, 280nm

8.  Liquid detection sensor : PCR setup Zone

9.  Barcode scanning: Primary tube

10. Accessories: T-rack, Cartridge racks, trash drawer, 96-well PCR rack, 24-well 1.5ml tube rack

Power Supply

Voltage: AC 200-240V; 

Frequency: 50/60Hz ; Max 7A, 1500W

Dimension

W1240*D920*H830 (mm) 

Net Weight 

280 Kg / 617 lbs

Net Weight (with accessories)

320 Kg / 706 lbs

Specification

━ Operating Parameters

Processing Capacity

1-48 samples (in batches of 24)

Processing Time

30-120 minutes (depends on sample type)

Sample Volume

200/400/1200/4000 µl (application dependent)

Elution Volume

40/60/100/150/200 µl

(application dependent)

Yield

200ul whole blood (average 6ug gDNA)

400ul whole blood (average 12ug gDNA)

Purity

DNA: O.D A

260

/A

280

 ratio 1.8±0.1

RNA: O.D A

230

/A

260

/A

280

 ratio 2.0±0.2

Pipetting Accuracy

Extraction Zone: 40µl <5%; 60µl <2%; 100-900µl <1.5% 

PCR setup Zone: 50–5 µl≤1%; 5–2 µl≤5%; 1µl≤10%

━ Operating Environment

Using Environment

indoor use

Temperatures Allowed During Operation

18~30°C

Temperatures Allowed During Transportation/Storage Packaging

15~40°C

Humidity Allowed During Operation

30~75%

Overvoltage category Category II

Pollution Degree Level 2

Sound level No Hazardous sound emission

━ Applications

Applications: Whole Blood DNA (200μl/400μl/1,200μl), Viral Nucleic Acid (DNA/RNA 200μl/400μl/1,200μl), FFPE Genomic DNA, 

FFPE Total RNA, Forensic DNA, Plasma and Circulating Cell-Free DNA (1,200μl/4,000μl), Tissue Genomic DNA, Plant Genomic 

DNA, Bacterial DNA, Whole Blood Total RNA, Cultured Cells Total RNA, Tissue Total RNA, Plant Total RNA, PCR Set-up.

Summary of Contents for EDA

Page 1: ...meters 5 OperatingEnvironment 5 Applications 5 Unpacking 6 EDAOnly 6 EDAStandSet 8 OpenLids 10 RemoveSafetyBrackets 11 Accessories 12 Azone 12 Bzone 12 Installation 13 SystemOverview 14 InstallMagCore...

Page 2: ...e not sure of the type of power supply at your lab consultthesellerorlocalpowercompany Forproductsintendedtooperatefrom batteryorothersources refertotheoperatinginstructions 11 Grounding or Polarizati...

Page 3: ...Foradetaileddescriptionofinstrumentsetup andhandling refertotheMagCore instrumentOperator sManual StoretheReagentCartridgeat 15to 25 Cbeforeuse Thekitmaynotworkifyouusethereagentsat temperaturesoutsi...

Page 4: ...sbufferarecompletelyinactivatedandnon infectious Itisrecommendedthatalldisposableplasticsareautoclavedordiscardedunderlocalregulations aftersampleprocessingiscompleted Donoteat drinkorsmokeinthelabora...

Page 5: ...tfor n tests Warning electricity Instructionmanualmustbereadbeforeoperating Consultinstructionsforuse Alternatecurrentinput Use byDate Nouseperiod Temperaturestorageconditions15 C 30 C Cataloguenumber...

Page 6: ...ProcessingTime 30 120minutes dependsonsampletype SampleVolume 200 400 1200 4000 l applicationdependent ElutionVolume 40 60 100 150 200 l applicationdependent Yield 200ulwholeblood average6uggDNA 400u...

Page 7: ...6 MagCore EDA Unpacking EDAOnly Cutoffallplastic packingbelts A Removethe cornerprotectors B Disassembleeach packingsides C...

Page 8: ...7 www rbcbioscience com Removethescrews alongthemetalbar onbothsidesofthe instrument D Removethefixing plateandmetalbars E Usethepallettruckor forklifttocarryEDAto theareayou veprepared F...

Page 9: ...8 MagCore EDA EDAStand Set Cutoffallplastic packingbelts A Removeallof the cornerprotectors B Disassembleeach packingsides C...

Page 10: ...9 www rbcbioscience com Takeouttherampsinside thestandingcabinet E Theloadingrampscan helpyouunloadtheEDA setatease F Removethescrews onthemetalfixing plateateachcorners D...

Page 11: ...10 MagCore EDA OpenLids Turnthelockpointto90 byusing ahexscrewdriver andthenyoucan takeoffthelids Besuretotakeoffthesidelids whenremovingfixingplate...

Page 12: ...ioscience com RemoveSafety Brackets A B E F G C D H E F G A B C D H A Piston out Azone B Piston side Azone C Piston out Bzone D Piston side Bzone E Drawer Azone F Spectrophotometer G Cooler EL ER H Dr...

Page 13: ...ipetteTipX20 10 MagCoreEDACuvette 10pcs X1 11 TrashCanwithTubeRackX1 12 BarcodeScannerX1 13 TypeAconnectorX1 1 2 3 4 5 7 6 8 9 10 11 12 13 YoucanpurchaseCat No P46040013 MSC100MagCoreCuvetteSet X100 f...

Page 14: ...the voltage of the outlet is between 220V and 240V Keep your hands dry Also make sure that the machine is turned off when you insert theplug Pluginthepowercordandturnonthemainpower Installation 1220mm...

Page 15: ...4 MagCore EDA System Overview PLCModule Mainboard Coolingfans TopServiceLid FrontDoor LogoIndicator OperationArea A Zone OperationArea Bzone Hepa Cooler PowerOutlet EthernetPort USBPort MainPowerSwitc...

Page 16: ...4oftheT Rack Insertthefrontofthecartridgeintothespace underthefixingrackofthecartridgeRack MakesuretoinserttheCartridge RackfirstbeforeT Rack W5 5mlTube W4 200 lSPTip W3 TipHolderset W2 DNase TipHolde...

Page 17: ...direction Pre coolingPremixReagentRackina 20 Cenvironment freezer Whentakenout itcanlastupto30minutes Thesurfacemightcondensesomewaterwhen takenoutfromthefreezer BesuretowipeoutextradropletsonthePremi...

Page 18: ...17 www rbcbioscience com BeforeStarting RemovethecapofUSBport LinkMagcoreEDAtocomputerwithprovidedTypeAconnector...

Page 19: ...entsavedprocess H Reset Resettodefaultstatus I EStop Emergencystopbutton J Link allzoneconnectioninstruction A H I G B C D E F Make sure to read the safety information to stop themachineproperlyincase...

Page 20: ...entkityouwantto use andselectthekitnumberonthesoftware page KitBarcodecanbefoundonthekitpackage Aftercompletingthescan clickonNexttocontinue theprocess 8 Thenumbercanbefoundin thestickeronthecartridge...

Page 21: ...toperformtheextraction Makesurethedooranddrawerareclosed 11 Thebuilt inscannerautomaticallyscansthe barcodeontheprimarytubes PulloutthedrawerinAzoneandslowlypushitin ClicktheAutoPrimaryScanoption TheE...

Page 22: ...lution thenplacethemontherack YouonlyhavetoselectPreMixAcheckbox PositiveandNegativecontrolwillshowinslot23and24 Standardwillshowintheslotbetween17 22 ThesetupNTCwillshowinslot12 Premixwater didnotset...

Page 23: ...platevolumeforyou YoucancheckthecheckboxtoactivateTemplate Mixataspirate MixatDispense 7 AtDeckLayout 1 4 draganddroptherackinto thedeck Clickonawellatthelasttippositionyou ve inserted Therackwillfill...

Page 24: ...ack Drag ReactionintoPCRrack 9 MakesurerefertothenumberdisplaysinEstimated Vol ul addtheliquidinthetubetothesameor highervolume andkeyintheactualvolumeinto ManualInput ul Makesurethedooranddrawerarecl...

Page 25: ...ectedtothemachine 1 2 ClicktheSerialDilutionbuttontomakethesetup 3 SetuptheconditionsforSamples Tubetypeand DilutionBlanks Selectsamplesinthelistandclickthedilute multiple The markmeansthatsomeunitsha...

Page 26: ...5 Thetiprackcanbedraggedanddroppedto positions1 4inthedecklayout Clickthelocationofthetipboxcontainingthetips bluecirclesindicatethatthereareavailabletips YoucanclickClear ifyouneedtoresettherackposit...

Page 27: ...neormoretipson theRack Makesurethedooranddrawerareclosed Confirmandcheckallyoursetup pressStartto execute 7 MakesurerefertothenumberdisplaysinEstimated Vol ul addtheliquidinthetubetothesameor highervo...

Page 28: ...to perform andselectthekitnumberonthesoftware page 3 Confirmyoursettings clickManualScan Barcodetocontinuetheprocess 4 ScanSampleorElution KitBarcodecanbefoundonthekitpackage Aftercompletingthescan cl...

Page 29: ...nthe barcode PullouttheAzonedrawer andthenslowlypushin 8 Confirmandcheckallyoursetup moveontoB ZonetosetupPCRassay ChooseoneofthethreemodefromManual PCR Kit andAutomatic 10 Formoredetailedinformationa...

Page 30: ...e morethanoneassay pleasegobacktoPremix ManualSetuppagetosetup 13 11 12 Youcanclickclearifyouneedtoresettherackposition Thetiprackcanbedraggedanddroppedto positions1 4inthedecklayout Clickthelocationo...

Page 31: ...heemptydeck IntheExtractandAssaySetup thesamples Template areinEL ERslots Drag ReactionintoPCRrack AftertheAandBzonesettingsarecompleted click ExecuteExtraction andtheEDAstartstoexecute thenucleicacid...

Page 32: ...thecartridge 3 Confirmyoursettings clickManualScan Barcodetocontinuetheprocess 4 ScanSampleorElution 6 UseBarcodeScannertoscanthebarcodeofthe stickeronthetube IftheSampleNumberSettingvalueis 24 waitfo...

Page 33: ...andkeyinDilute tosameconcentrationvalueyouneed Thebuilt inscannerwillautomaticallyscanthe barcodeontheprimarytubes PulloutthedrawerinAzoneandslowlypushitin 8 ConfirmandcheckallyoursettingsinAzoneand...

Page 34: ...kthelocationofthetipboxcontainingthetips bluecirclesindicatethatthereareavailabletips Ifyouwantthesamplesolutiontoperformmixing actions youcanchecktheboxintheform Clickthe Assay thenenterthetotalreact...

Page 35: ...wer the Tip requirement you need to go back to Deck Layout 1 4 to add oneormoretipsontheRack Makesurethedooranddrawerareclosed Whenallyoursetupwerecheckup pressContinue tostartfinalassayprocess 15 14...

Page 36: ...hedoorisclosed youcandecideto continueoraborttheprogress Duringprogramprocessing youcanpauseor abortthecurrentexecution PressPauseandthemachinesuspendsoperation immediately ClickAbort tocancelallprogr...

Page 37: ...eorBZone Youcancheckthesystemstatusonthesettingpage System EjectTip Onthesettingpage youcancheckthesystem ejectthetip andmaintaintheEDAdevice Usersneedtoperformthesechecksregularlyto ensurethesystemis...

Page 38: ...bnormalsituationoccurs youcanpauseandabortthe movementprocess ThefollowingstepsforreplacingtheO ring 1 RemovethedamagedO Ring 2 ReplacetheO ringwithanewone 3 ApplygreasetotheO ringandperformthepistont...

Page 39: ...dandwhetherthetipcanbe takenoutfromtheT Racksmoothly SelectthewelloftheT rackwherethetip wolud liketobetested 2 Followthesteps thenclickNexttocontinuethe test Hereisanexampleofthepistontestbyclicking...

Page 40: ...ndabortthemovementprocessifthere san abnormalsituation 1 SelectHeater CoolerTesttostartthetesting procedure 1 TherearetwotemperaturesforAzone sheating andoneforcoolingsystem Beforetestingtheheater ple...

Page 41: ...ordcanbe providedtoengineerssothattheycandetermine thecauseofmachinefailure Themachinecanrecordthealarminghistory happeningintheAzoneorBzoneseparately Thealarmrecordcanauto generateandsaveintheEDAsyst...

Page 42: ...41 www rbcbioscience com MagCore EDA...

Page 43: ...42 MagCore EDA...

Page 44: ...15F No 15 QiaoheRd ZhongheDist NewTaipeiCity235029 Taiwan TEL 886289121200FAX 86289121300...

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