MagCore EDA Operation Manual Download Page 20

19

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A Zone - Nucleic Acid Extration

━ Start Programs

3

Log in to the EDA operating application.

1

Make sure the link shows green light to indicate that 

it is connected to the machine.

Click the 

Nucleic Acid Extration

 

button to start 

the extraction setting.

2

Confirm your settings, click 

Manual Scan 

Barcode 

to continue the process.

6

4

Confirm the code of the reagent kit you want to 

use, and select the kit number on the software 

page.

Kit Barcode 

can be found on the kit package.

After completing the scan, click on 

Next

 to continue 

the process.

8

!

The number can be found in 

the sticker on the cartridge.

Setup all the required conditions and press 

Next

 to 

continue.

5

*  When setting 

Sample Number

, if value >24, then the extraction will 

be going to two run(1~24, 25~48) for the same condition.

Scan 

Sample

 or 

Elution

Use Barcode Scanner to scan the barcode of the 

sticker on the tube.

7

*  If the 

Sample Number Setting

 value is >24, wait for procedure goes 

to second run, then you can scan 25~48 tubes.

Summary of Contents for EDA

Page 1: ...meters 5 OperatingEnvironment 5 Applications 5 Unpacking 6 EDAOnly 6 EDAStandSet 8 OpenLids 10 RemoveSafetyBrackets 11 Accessories 12 Azone 12 Bzone 12 Installation 13 SystemOverview 14 InstallMagCore...

Page 2: ...e not sure of the type of power supply at your lab consultthesellerorlocalpowercompany Forproductsintendedtooperatefrom batteryorothersources refertotheoperatinginstructions 11 Grounding or Polarizati...

Page 3: ...Foradetaileddescriptionofinstrumentsetup andhandling refertotheMagCore instrumentOperator sManual StoretheReagentCartridgeat 15to 25 Cbeforeuse Thekitmaynotworkifyouusethereagentsat temperaturesoutsi...

Page 4: ...sbufferarecompletelyinactivatedandnon infectious Itisrecommendedthatalldisposableplasticsareautoclavedordiscardedunderlocalregulations aftersampleprocessingiscompleted Donoteat drinkorsmokeinthelabora...

Page 5: ...tfor n tests Warning electricity Instructionmanualmustbereadbeforeoperating Consultinstructionsforuse Alternatecurrentinput Use byDate Nouseperiod Temperaturestorageconditions15 C 30 C Cataloguenumber...

Page 6: ...ProcessingTime 30 120minutes dependsonsampletype SampleVolume 200 400 1200 4000 l applicationdependent ElutionVolume 40 60 100 150 200 l applicationdependent Yield 200ulwholeblood average6uggDNA 400u...

Page 7: ...6 MagCore EDA Unpacking EDAOnly Cutoffallplastic packingbelts A Removethe cornerprotectors B Disassembleeach packingsides C...

Page 8: ...7 www rbcbioscience com Removethescrews alongthemetalbar onbothsidesofthe instrument D Removethefixing plateandmetalbars E Usethepallettruckor forklifttocarryEDAto theareayou veprepared F...

Page 9: ...8 MagCore EDA EDAStand Set Cutoffallplastic packingbelts A Removeallof the cornerprotectors B Disassembleeach packingsides C...

Page 10: ...9 www rbcbioscience com Takeouttherampsinside thestandingcabinet E Theloadingrampscan helpyouunloadtheEDA setatease F Removethescrews onthemetalfixing plateateachcorners D...

Page 11: ...10 MagCore EDA OpenLids Turnthelockpointto90 byusing ahexscrewdriver andthenyoucan takeoffthelids Besuretotakeoffthesidelids whenremovingfixingplate...

Page 12: ...ioscience com RemoveSafety Brackets A B E F G C D H E F G A B C D H A Piston out Azone B Piston side Azone C Piston out Bzone D Piston side Bzone E Drawer Azone F Spectrophotometer G Cooler EL ER H Dr...

Page 13: ...ipetteTipX20 10 MagCoreEDACuvette 10pcs X1 11 TrashCanwithTubeRackX1 12 BarcodeScannerX1 13 TypeAconnectorX1 1 2 3 4 5 7 6 8 9 10 11 12 13 YoucanpurchaseCat No P46040013 MSC100MagCoreCuvetteSet X100 f...

Page 14: ...the voltage of the outlet is between 220V and 240V Keep your hands dry Also make sure that the machine is turned off when you insert theplug Pluginthepowercordandturnonthemainpower Installation 1220mm...

Page 15: ...4 MagCore EDA System Overview PLCModule Mainboard Coolingfans TopServiceLid FrontDoor LogoIndicator OperationArea A Zone OperationArea Bzone Hepa Cooler PowerOutlet EthernetPort USBPort MainPowerSwitc...

Page 16: ...4oftheT Rack Insertthefrontofthecartridgeintothespace underthefixingrackofthecartridgeRack MakesuretoinserttheCartridge RackfirstbeforeT Rack W5 5mlTube W4 200 lSPTip W3 TipHolderset W2 DNase TipHolde...

Page 17: ...direction Pre coolingPremixReagentRackina 20 Cenvironment freezer Whentakenout itcanlastupto30minutes Thesurfacemightcondensesomewaterwhen takenoutfromthefreezer BesuretowipeoutextradropletsonthePremi...

Page 18: ...17 www rbcbioscience com BeforeStarting RemovethecapofUSBport LinkMagcoreEDAtocomputerwithprovidedTypeAconnector...

Page 19: ...entsavedprocess H Reset Resettodefaultstatus I EStop Emergencystopbutton J Link allzoneconnectioninstruction A H I G B C D E F Make sure to read the safety information to stop themachineproperlyincase...

Page 20: ...entkityouwantto use andselectthekitnumberonthesoftware page KitBarcodecanbefoundonthekitpackage Aftercompletingthescan clickonNexttocontinue theprocess 8 Thenumbercanbefoundin thestickeronthecartridge...

Page 21: ...toperformtheextraction Makesurethedooranddrawerareclosed 11 Thebuilt inscannerautomaticallyscansthe barcodeontheprimarytubes PulloutthedrawerinAzoneandslowlypushitin ClicktheAutoPrimaryScanoption TheE...

Page 22: ...lution thenplacethemontherack YouonlyhavetoselectPreMixAcheckbox PositiveandNegativecontrolwillshowinslot23and24 Standardwillshowintheslotbetween17 22 ThesetupNTCwillshowinslot12 Premixwater didnotset...

Page 23: ...platevolumeforyou YoucancheckthecheckboxtoactivateTemplate Mixataspirate MixatDispense 7 AtDeckLayout 1 4 draganddroptherackinto thedeck Clickonawellatthelasttippositionyou ve inserted Therackwillfill...

Page 24: ...ack Drag ReactionintoPCRrack 9 MakesurerefertothenumberdisplaysinEstimated Vol ul addtheliquidinthetubetothesameor highervolume andkeyintheactualvolumeinto ManualInput ul Makesurethedooranddrawerarecl...

Page 25: ...ectedtothemachine 1 2 ClicktheSerialDilutionbuttontomakethesetup 3 SetuptheconditionsforSamples Tubetypeand DilutionBlanks Selectsamplesinthelistandclickthedilute multiple The markmeansthatsomeunitsha...

Page 26: ...5 Thetiprackcanbedraggedanddroppedto positions1 4inthedecklayout Clickthelocationofthetipboxcontainingthetips bluecirclesindicatethatthereareavailabletips YoucanclickClear ifyouneedtoresettherackposit...

Page 27: ...neormoretipson theRack Makesurethedooranddrawerareclosed Confirmandcheckallyoursetup pressStartto execute 7 MakesurerefertothenumberdisplaysinEstimated Vol ul addtheliquidinthetubetothesameor highervo...

Page 28: ...to perform andselectthekitnumberonthesoftware page 3 Confirmyoursettings clickManualScan Barcodetocontinuetheprocess 4 ScanSampleorElution KitBarcodecanbefoundonthekitpackage Aftercompletingthescan cl...

Page 29: ...nthe barcode PullouttheAzonedrawer andthenslowlypushin 8 Confirmandcheckallyoursetup moveontoB ZonetosetupPCRassay ChooseoneofthethreemodefromManual PCR Kit andAutomatic 10 Formoredetailedinformationa...

Page 30: ...e morethanoneassay pleasegobacktoPremix ManualSetuppagetosetup 13 11 12 Youcanclickclearifyouneedtoresettherackposition Thetiprackcanbedraggedanddroppedto positions1 4inthedecklayout Clickthelocationo...

Page 31: ...heemptydeck IntheExtractandAssaySetup thesamples Template areinEL ERslots Drag ReactionintoPCRrack AftertheAandBzonesettingsarecompleted click ExecuteExtraction andtheEDAstartstoexecute thenucleicacid...

Page 32: ...thecartridge 3 Confirmyoursettings clickManualScan Barcodetocontinuetheprocess 4 ScanSampleorElution 6 UseBarcodeScannertoscanthebarcodeofthe stickeronthetube IftheSampleNumberSettingvalueis 24 waitfo...

Page 33: ...andkeyinDilute tosameconcentrationvalueyouneed Thebuilt inscannerwillautomaticallyscanthe barcodeontheprimarytubes PulloutthedrawerinAzoneandslowlypushitin 8 ConfirmandcheckallyoursettingsinAzoneand...

Page 34: ...kthelocationofthetipboxcontainingthetips bluecirclesindicatethatthereareavailabletips Ifyouwantthesamplesolutiontoperformmixing actions youcanchecktheboxintheform Clickthe Assay thenenterthetotalreact...

Page 35: ...wer the Tip requirement you need to go back to Deck Layout 1 4 to add oneormoretipsontheRack Makesurethedooranddrawerareclosed Whenallyoursetupwerecheckup pressContinue tostartfinalassayprocess 15 14...

Page 36: ...hedoorisclosed youcandecideto continueoraborttheprogress Duringprogramprocessing youcanpauseor abortthecurrentexecution PressPauseandthemachinesuspendsoperation immediately ClickAbort tocancelallprogr...

Page 37: ...eorBZone Youcancheckthesystemstatusonthesettingpage System EjectTip Onthesettingpage youcancheckthesystem ejectthetip andmaintaintheEDAdevice Usersneedtoperformthesechecksregularlyto ensurethesystemis...

Page 38: ...bnormalsituationoccurs youcanpauseandabortthe movementprocess ThefollowingstepsforreplacingtheO ring 1 RemovethedamagedO Ring 2 ReplacetheO ringwithanewone 3 ApplygreasetotheO ringandperformthepistont...

Page 39: ...dandwhetherthetipcanbe takenoutfromtheT Racksmoothly SelectthewelloftheT rackwherethetip wolud liketobetested 2 Followthesteps thenclickNexttocontinuethe test Hereisanexampleofthepistontestbyclicking...

Page 40: ...ndabortthemovementprocessifthere san abnormalsituation 1 SelectHeater CoolerTesttostartthetesting procedure 1 TherearetwotemperaturesforAzone sheating andoneforcoolingsystem Beforetestingtheheater ple...

Page 41: ...ordcanbe providedtoengineerssothattheycandetermine thecauseofmachinefailure Themachinecanrecordthealarminghistory happeningintheAzoneorBzoneseparately Thealarmrecordcanauto generateandsaveintheEDAsyst...

Page 42: ...41 www rbcbioscience com MagCore EDA...

Page 43: ...42 MagCore EDA...

Page 44: ...15F No 15 QiaoheRd ZhongheDist NewTaipeiCity235029 Taiwan TEL 886289121200FAX 86289121300...

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