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Lucetta™ 2 Luminometer Manual – 

Bioscience Solutions

   15

9.2 Technical Data

Sample and Data Handling

Sample Tube Format

Transparent polystyrol tubes and microcentrifuge tubes 

Diameter: maximum up to 15 mm; minimum approximately 12 mm Length: 47-75 mm

Detector

Ultra fast single photon counter with spectral sensitivity range of 380-630 nm

Sensitivity

1 fmol ATP using the ATP Bioluminescence Assay Kit CLSII (Boehringer Mannheim, FRG)

Background Value

Background average <20 RLU/s

Dynamic Range

6 orders of magnitude

Measurement Time

1–999 sec, in increments of 1 sec

Data Format

Data output in RLU

Hardware

Microcontroller to control all instrument functions

Microprocessor Software

8 buttons: 1 <ON/LIGHT> button, 1 <OFF> button, 1 <OK> button, 1 <ESC> button, 4 navigation buttons 

,

,

, and 

 .

Measurement of glow luminescence

2 operating modes (one pre-tailored for MycoAlert™ / MycoAlert™ PLUS Mycoplasma Detection Assay, one for single read measurement)

Display 

Graphic display with backlit LED, 128 x 64 dots

Interfaces

USB 2.0 Type B USB interface for PC connection (data transfer)

Software

Built-in microprocessor, storage of 6 different customized measuring protocols

Data storage

Storage of the last 2000 results

Hardware and Software Requirements 

for Data Upload (Optional)

PC with industrial standard, Microsoft® Windows® 7 (32/64 bit) or higher Microsoft® EXCEL®

PC-software

Data capture software program Lucetta™ Connect for data transfer to Excel® 

Instrument Specifications

Dimensions (w x d x h)

150 mm x 280 mm x 170 mm (5.90 in x 11.02 in x 6.69 in)

Weight

Approx. 2 kg (4.4 lbs), incl. batteries

Power supply

Universal input: 100–240 V AC, 50–60 Hz

Output:

5.9–6 V DC, 2.1 A (Negative pole is on the inside of the plug!)

Protection class

II

Power consumption (instrument)

Max. 15 W

Batteries 

3 rechargeable Ni/MH Akku Baby, 1.2 V / 4000 mAh

Temperature range and humidity

Storage: 5°–40° C, Operation: 15°–30°C 80% humidity at 31°C degreasing linearly to 50% at 40°C, no condensation

Electrical safety 

IEC 61010-1:2010 (Third Edition) UL 61010-1 (Third Edition) (USA) CAN/CSA-C22.2 No. 61010-1-12 (Third Edition) (Canada)

Certification

This instrument bears the CE mark, based on conformity to EU legislation: Electromagnetic compatibility (EMC) 2014/35/EU, Low 

voltage (LVD) 2014/35/EU, Restriction of Hazardous Substances (RoHS) 2011/65/EG

Manufacturing date

The manufacturing year is encoded by the second and third digit of the serial number, e.g. an instrument with a serial number 

719L2xxx was manufactured in 2019.

 

Summary of Contents for Lucetta 2

Page 1: ...Bioscience Solutions Lucetta 2 Luminometer Manual...

Page 2: ......

Page 3: ...ctions 5 2 2 Maintenance 5 2 3 Safety Instructions and Use Precautions 6 2 4 Storage Conditions 6 2 5 Batteries 6 2 6 Return Shipment 7 2 7 Instrument Disposal 7 3 0 Setup Instructions 7 3 1 Lucetta 2...

Page 4: ...ta Storage and PC Upload 11 5 1 Maximum Storage Capacity 11 5 2 Viewing Logged Measurements 11 5 3 Upload to a PC 11 6 0 Setting Instrument Parameters 12 6 1 Password 12 6 2 Customer Settings 12 6 3 D...

Page 5: ...abled by the Lucetta Connect software Broad range of applications Lucetta 2 Luminometer applications include MycoAlert PLUS and MycoAlert Mycoplasma Detection Assays ATP assays e g ViaLight Cell Proli...

Page 6: ...strument is not approved for use in fire or explosion potential areas nor for use with flammable or explosive media The sample holder must remain free of dust and contamination The holder should only...

Page 7: ...tance if required 2 7 Instrument Disposal Decontaminate the instrument before disposal This luminometer contains electronic parts To prevent environmental pollution please dispose of the instrument an...

Page 8: ...nd the age of the battery The battery may auto discharge when the instrument is not used for an extended period To optimize the battery lifespan the display light switches off after 15 sec if the inst...

Page 9: ...from 0 to 9 Press OK The instrument will first perform reading A for all samples and then continue with reading B for all samples For convenient handling of incubation times we recommend a maximum num...

Page 10: ...efine a new protocol 4 Each measurement is given an identifier number displayed under TEST By default the number begins with 1 counting upwards The number can be customized by using the arrow buttons...

Page 11: ...ns to select day month or year Depending on the selected country for the date format for more details see section 6 0 the format is MM DD YYYY USA or DD MM YYYY Europe Theflashingcursorindicatesthenum...

Page 12: ...word for a new instrument is 0000 Maximum number of digits for a password is four 1 Navigate down to SETTINGS and press OK 2 Select PASSWORD and press OK 3 Enter OLD PASSWORD To change the given numbe...

Page 13: ...Figure 13 Display for setting date and time 6 4 System Display brightness and contrast are adaptable to the lighting conditions 1 Navigate down to SETTINGS and press OK 2 Select SYSTEM and press OK 3...

Page 14: ...liance with operating conditions Background or measurement value too low 0 RLU s Detector or electronics damaged or incorrect instrument settings Service required please contact Lonza Unexpected or wr...

Page 15: ...asuring protocols Data storage Storage of the last 2000 results Hardware and Software Requirements for Data Upload Optional PC with industrial standard Microsoft Windows 7 32 64 bit or higher Microsof...

Page 16: ...22 75323 Bad Wildbach Germany and distributed by Lonza Cologne GmbH Nattermannallee 1 50829 Cologne Germany and Lonza Walkersville Inc 8830 Biggs Ford Road Walkersville MD 21793 Microsoft Windows and...

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