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LI-COR ODYSSEY Sa, Operator'S Manual

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LI-COR ODYSSEY Sa Operator'S Manual Download Page 10

Chapter 1: Safety and Operational Information

Safety Interlocks

There are no user serviceable parts inside the Odyssey

®

Sa Imager and it is not recommended that

users open the instrument enclosure.

When the top panel of the instrument is opened, safety interlocks immediately turn off the lasers.
Audible tones also sound when the top panel of the enclosure is opened and closed.

Figure 4-1. Back panel label of the Odyssey Sa Imager showing the following: 1) Power requirements, 2) FCC and
CDRH compliance notices, 3) Laser radiation exposure warning, and 4) Class 1 Laser Product classification.

Chemical Safety

LI-COR Biosciences recommends that all biochemicals be handled carefully, and that safe laboratory
procedures be followed at all times. Be aware of the hazards associated with any chemical before you
begin work.

The Odyssey Sa Imager should not be used with any radioactive materials.

Page 10

Summary of Contents for ODYSSEY Sa

Page 1: ... Operator s Manual ...

Page 2: ......

Page 3: ...This product model number 9260 is a CE marked product For conformity information contact LI COR Support at http www licor com biotechsupport Outside of the U S contact your local sales office or distributor Page 3 ...

Page 4: ...ary for you to refer to the manual or accompanying documents in order to protect against damage to the product The product is marked with this symbol when a hazardous voltage may be present Manual Markings WARNING Warnings must be followed carefully to avoid bodily injury CAUTION Cautions must be observed to avoid damaging your equipment NOTE Notes contain additional information and useful tips IM...

Page 5: ...ompany 2015 LI COR Inc Specifications subject to change LI COR Odyssey Image Studio and IRDye are registered trademarks of LI COR Inc in the United States and other countries All other trademarks belong to their respective owners The Odyssey Infrared Imaging Systems and IRDye reagents are covered by U S patents foreign equivalents and patents pending Federal Communications Commission Radio Frequen...

Page 6: ...ciences GmbH Siemensstraße 25A 61352 Bad Homburg Germany Phone 49 0 6172 17 17 771 Fax 49 0 6172 17 17 799 bio eu licor com LI COR Biosciences UK Ltd St John s Innovation Centre Cowley Road Cambridge CB4 0WS United Kingdom Phone 44 0 1223 422104 Fax 44 0 1223 422105 bio eu licor com LI COR Biotechnology Distributor Network www licor com bio distributors Page 6 ...

Page 7: ... Replacement 12 Routine Maintenance 12 Chapter 2 Overview and General Description Buttons and Indicator Lights 15 Power Button 15 Using the Power Button to Reset the Odyssey Sa Imager 15 Eject Button 15 Using the Eject Button to Reset Network Parameters 16 Indicator Lights 16 Back Panel Connectors 16 Hardware Description 16 Scanning and Detection Hardware 16 Odyssey Sa Server and Server Software 1...

Page 8: ...Automatic IP Addressing 27 Reset to Automatic IP Addressing 28 Chapter 5 Installing a BioTek BioStack Plate Stacker Introduction 31 Installation Summary 31 Initial Odyssey Sa Setup 32 Initial BioStack Setup 32 Connecting Power and Communication Cables 34 Leveling the Odyssey Sa Imager 35 Installing the Locator Clamp 36 Leveling the BioStack Plate Stacker 37 Connecting the BioStack Plate Stacker to...

Page 9: ...nstrument Radiation emitted inside the instrument is confined within protective housings and external covers The laser beam cannot escape during any phase of user operation The CDRH implemented regulations for laser products on August 1 1976 CDRH radiation performance standard 21 Code of Federal Regulations Chapter 1 Subchapter J Compliance for products marketed in the United States is mandatory T...

Page 10: ... enclosure is opened and closed Figure 4 1 Back panel label of the Odyssey Sa Imager showing the following 1 Power requirements 2 FCC and CDRH compliance notices 3 Laser radiation exposure warning and 4 Class 1 Laser Product classification Chemical Safety LI COR Biosciences recommends that all biochemicals be handled carefully and that safe laboratory procedures be followed at all times Be aware o...

Page 11: ... 22 inches and a vertical clearance of at least 37 cm 14 When used with the BioTek BioStack microplate stacking system the area required is approximately 61 x 122 cm 24 x 48 Removing the Laser Microscope Assembly Shipping Lock The laser microscope assembly moves beneath the microplate when the Odyssey Sa Imager is scanning It is important to lock this assembly in place during shipment to prevent a...

Page 12: ... the instruments plugged into the extension cord does not exceed the extension cord ampere rating Also make sure the total amperage of instruments plugged into the wall outlet does not exceed the amperage capacity for the outlet In the U S this is usually 15 or 20 amperes Fuse Replacement There are no user serviceable fuses inside the Odyssey Sa Imager If the instrument fails to power up and you s...

Page 13: ...Odyssey Sa Imager carbon tetrachlorides lacquer thinner or alcohol to clean the case l Inspect all cables and power cords for evidence of fraying exposed wire or loose connections Page 13 ...

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Page 15: ...omes unresponsive the instrument can be reset by pressing and holding in the power button for five seconds This procedure cuts power to the instrument and internal computer so it should only be used when communication cannot be established by any other means Normal operation should resume after the power button is pressed again to turn the instrument back on though start up may take several minute...

Page 16: ...Odyssey Sa System on page 25 The receptacle for the power cable is also located on the back panel Note The power switch for the power supply on the back panel should always be in the ON position For normal operation the instrument should always be powered on and off from the power switch on the instrument front panel Hardware Description Scanning and Detection Hardware The Odyssey Sa Imager uses t...

Page 17: ...Studio Software The Odyssey Sa server has a pre installed copy of Odyssey Sa Server software This software is periodically updated with new releases that are posted on LI COR Biosciences web site Users will be contacted with download instructions when new releases are available The Odyssey Sa server communicates with the computer via TCP IP in a stand alone configuration When a scan is started fro...

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Page 19: ...and deep well microplates conforming to the following standards ANSI SBS 1 2004 Microplates Footprint Dimensions ANSI SBS 2 2004 Microplates Height Dimensions ANSI SBS 3 2004 Microplates Bottom Outside Flange Dimensions ANSI SBS 4 2004 Microplates Well Positions Note The ANSI SBS standards can be found on the Internet at http www sbsonline org The ANSI SBS standards specify standard dimensions for...

Page 20: ...ned by scanning a plate containing experimental and control samples at 1 7 2 0 3 0 and 4 0 mm focus offsets Use the same intensity settings for each scan After reviewing the collected scans use the focus offset with the highest signal to noise for experiments Scanning Microplates Loading Microplates for Standard Scans Before scanning clean the bottom plate surface with a moist lint free paper to r...

Page 21: ...an time will increase More specific scanning guidelines may also be given in the various protocols in the Odyssey Sa Imager Application Protocols manual Scanning Membranes in the Membrane Carrier The Membrane Carrier allows for scanning blots or gels up to 2 13 16 x 4 1 2 7 2 x 11 4 cm in size The Membrane Carrier has the same outer dimensions as a standard microplate but with a ridge around the e...

Page 22: ...nt front panel to open the microplate carrier Place the Membrane Carrier into the microplate carrier and press the eject button again to close the micro plate carrier Scanning starts on the side where the A1 marker is located on the Odyssey Sa plate carrier If the membrane carrier is placed with the top of the membrane toward the A1 marker you will get an image on the display that is oriented corr...

Page 23: ... Code Reader Optional Accessory When a scan is started the Odyssey Sa bar code reader automatically scans the side of the microplate for a bar code If a compatible bar code is found it is read and stored in memory To be compatible the characters used in the bar code must be acceptable as file name characters These include numerals upper and lower case alphabet space underscore and period The Image...

Page 24: ...f the supported bar code symbologies that will fit these dimensions and the minimum bar code line width of at least 0 177 mm Code 39 17 characters Codabar 18 characters Code 128 30 characters numeric only 18 characters as listed on p 13 Code 93 14 characters If a narrower bar code is used the number of characters must also be reduced so that the minimum line width of the bar code is at least 0 177...

Page 25: ...ey Sa Express software follow the directions below to add the software to Image Studio 1 Insert the Odyssey Sa Express software key CD 2 Click the Application Menu Button and click Import Key 3 Browse to the Odyssey Sa Express Software key file on the CD and click Open 4 Click OK in the pop up window and restart Image Studio software Assembling the Odyssey Sa System Since this chapter is not an in...

Page 26: ...es 6 To confirm that Image Studio Software can communicate with the Odyssey Sa Imager check that the buttons on the Acquire ribbon are active and the status submenu in the ribbon says Ready The instrument will be listed by serial number if connected Select it and click OK If it s not lis ted click on Auto Discover to run the autodiscovery process again It may take several minutes for the Odyssey S...

Page 27: ...ssword in a safe place Adding Users User account information is stored on the Odyssey Sa Imager rather than on the computer If you do not have a user account you will need to create one 8 Open the Application menu hover over Instrument hover over Management and click Admin istration 9 Click Manage Users to set up a new user account 10 Close the System Administration window when you are through add...

Page 28: ...cases use Automatic addressing for the Odyssey Sa Imager Reset to Automatic IP Addressing If you cannot connect to your Odyssey Sa Imager it is possible that it is configured to an unknown manual address It is necessary in this case to reset the network configuration to automatic addressing by following the steps below Page 28 ...

Page 29: ... Press the power button on the Odyssey Sa Imager It will start blinking to show that the instru ment is booting 6 Press and hold the eject button during the boot process when the green scanning status light is blinking Make sure the eject button is depressed at the end of the four times that the green scan ning status light blinks The networking is now configured to automatic addressing and you ca...

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Page 31: ...yssey Sa Imager It also includes communication cables to connect the two instruments The components of the installation kit are shown below Components 1 BioStack stand 2 Leveling and alignment fixture with storage thumb screw 3 Thumbscrews to connect the BioStack Plate Stacker to the stand 4 Locator clamp 5 USB cable and calibration ruler not pictured Installation Summary 1 Connect the BioStack Pl...

Page 32: ... and the weight of the combined systems A sturdy surface is also a requirement Both the Odyssey Sa Imager and the BioStack Plate Stacker must be level during operation Any surface that is susceptible to movement during operation is not a good choice for the Odyssey Sa BioStack combination l Before starting installation each leveling foot on the Odyssey Sa Imager should be turned out one turn Note ...

Page 33: ...ent avant la fin de l installation 3 Orient the BioStack stand so the end with the two large knobs front faces toward the front of the Odyssey Sa Imager Orient the BioStack instrument so the side with the plate gripper also faces toward the Odyssey Sa Imager 4 Place the BioStack Plate Stacker on the stand with the plate gripper facing the front of the stand and the back of the BioStack Plate Stack...

Page 34: ...tack Plate Stacker to the stand using four thumb screws Connecting Power and Communication Cables 6 Connect one end of the USB cable into one of the Odyssey Sa Imager USB ports 7 Connect the other end of the USB cable to the USB port on the BioStack Plate Stacker Page 34 ...

Page 35: ...d on before the Odyssey Sa Imager 11 Turn on the Odyssey Sa Imager The Odyssey Sa Imager should be turned on for all remaining steps Leveling the Odyssey Sa Imager 12 If necessary move the BioStack Plate Stacker so there is at least 18 cm 7 in clearance between the Odyssey Sa Imager and the BioStack plate gripper If there is insufficient clearance the extended Odyssey Sa Imager plate carrier will ...

Page 36: ...ect button to retract the plate carrier Installing the Locator Clamp A locator clamp is provided that locks around the front legs of the Odyssey Sa Imager In later steps the BioStack stand will be connected to the locator clamp which locks both the Odyssey Sa and the BioStack Plate Stacker in position 17 Make sure the clamp is open before starting Flip the latch cover up and rotate the latch until...

Page 37: ...ker 21 Push the eject button on the Odyssey Sa Imager front panel to extend the plate carrier 22 Move the BioStack Plate Stacker directly in front of the Odyssey Sa Imager Be careful not to strike the Odyssey Sa Imager plate carrier 23 Look between the instruments with your line of sight level with the Odyssey Sa Imager plate car rier Adjust the leveling feet on the BioStack stand so the Odyssey S...

Page 38: ...ect button on the Odyssey Sa Imager front panel to retract the plate carrier 25 Turn off the BioStack Plate Stacker 26 Slide the leveling and alignment fixture into the BioStack plate gripper and make sure it rests flat on the gripper arms The A1 corner of the fixture should be in the same orientation that it was in the Odyssey Sa Imager plate carrier Push the fixture all the way in until it conta...

Page 39: ...ract the plate carrier Remove the leveling and alignment fixture from the plate gripper Connecting the BioStack Plate Stacker to the Locator The BioStack stand has two large knobs on the front of the stand that tighten two alignment pins These alignment pins fit into the two holes on the locator clamp The steps below can be used to adjust the height of the alignment pins and to connect the BioStac...

Page 40: ...ded washer below the stand and unscrew the knob until it comes off While holding the threaded washer screw the align ment pin in or out as needed to adjust the height and then replace the knob but don t tighten it 33 Lift the front of the BioStack stand and guide the alignment pins into the holes in the locator clamp Page 40 ...

Page 41: ...Stacker is now close to the final position but some small movements will be needed in the X Y dimensions to ensure that microplates are placed squarely on the plate carrier 36 With the leveling and alignment fixture still in the BioStack plate gripper manually lower the plate gripper until it is just above the Odyssey Sa Imager plate carrier Make sure the BioStack stacker is off 37 Observe the pos...

Page 42: ...e Stacker carefully and only in small increments Excessive movement can stress the plate gripper assembly potentially causing damage 39 Observe the bubble level on the fixture and make any necessary adjustments to level the BioStack Plate Stacker 40 Tighten the knob on both alignment pins You may need to hold the threaded washer until the knob begins to tighten 41 Manually raise the BioStack plate...

Page 43: ... on the Z axis is too small the plate will drop onto the Odyssey Sa Imager plate carrier rather than being placed on it If the value of the vertical travel distance is too large the plate will hit the Odyssey Sa Imager plate carrier and the plate gripper arms will continue to travel downward which could result in the bottom of the gripper assembly impacting the microplate as it rests on the Odysse...

Page 44: ... Plate Stacker 46 Click Z Axis Training in the BioTek BioStack window 47 Click Start in the Z axis training window This moves the BioStack plate gripper to its highest vertical position and opens the Odyssey Sa Imager plate carrier Page 44 ...

Page 45: ...e Amount mm field or use the arrows to set a value Three millimeters are added because the gripper arms should travel just below the upper surface of the Odyssey Sa Imager plate carrier 50 Click Move Down The bottom edge of the gripper should travel slightly below the top surface of the Odyssey Sa Imager plate carrier and the bend in the gripper arm that a plate normally rests on should be slightl...

Page 46: ...e Studio software expects microplates on the plate carrier to be in a certain orientation If the input stacks are loaded randomly some images will be right side up and others upside down Note The Odyssey Sa Imager membrane carrier is not compatible with the BioStack Plate Stacker In Image Studio Software you must start an express run to use the BioStack Plate Stacker To start an express run you mu...

Page 47: ...ate you will need to define a new express run for the plates remaining in the input stack In general you will have the least difficulty with jams and other problems related to the BioStack Plate Stacker if you use the recommended plates Chapter 3 and don t mix different types of plates in the input stack Another category of errors that can occur are communication errors between the Odyssey Sa Imag...

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Page 49: ...re 15 35 C and dew point not greater than 19 C non condensing altitude not to exceed 2000 m Environmental Conditions Pollution Degree 1 Fuse Ratings Fuses are non user replaceable A 5 A fast blow fuse protects the Digital Signal Processor DSP Power Requirements Automatic voltage selection between 100 127 VAC and 200 240 VAC voltage fluctuations not to exceed 10 of the nominal voltage 47 63 Hz 1 1 ...

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