6
10.2.5
The Pinhole and Its Effects............................................................................. 93
10.2.6
Image Detail and Raster Settings................................................................... 95
10.2.7
Signal and Noise ............................................................................................ 99
10.2.8
Profile Cuts................................................................................................... 101
10.3
Multiparameter Fluorescence......................................................................... 102
10.3.1
Illumination ................................................................................................... 102
10.3.2
Beam Splitting .............................................................................................. 104
10.3.3
Emission Bands............................................................................................ 104
10.3.4
Crosstalk ...................................................................................................... 104
10.3.5
Sequential Scanning .................................................................................... 105
10.3.6
Unmixing ...................................................................................................... 105
10.4
3D Series........................................................................................................ 106
10.4.1
Z-stack.......................................................................................................... 106
10.4.2
Section Thicknesses .................................................................................... 106
10.4.3
Distances...................................................................................................... 107
10.4.4
Data Volumes............................................................................................... 107
10.4.5
Depictions..................................................................................................... 108
10.4.5.1
Gallery ..................................................................................................... 108
10.4.5.2
Movie ....................................................................................................... 108
10.4.5.3
Orthogonal Projections ............................................................................ 109
10.4.5.4
Rotated Projections ................................................................................. 110
10.5
Time Series .................................................................................................... 110
10.5.1
Scan Speed.................................................................................................. 110
10.5.2
Points ........................................................................................................... 111
10.5.3
Lines............................................................................................................. 111
10.5.4
Planes .......................................................................................................... 111
10.5.5
Spaces (Time-Space)................................................................................... 111
10.5.6
FRAP Measurements ................................................................................... 112
10.6
Spectral Series ............................................................................................... 112
10.6.1
Data Acquisition and Utilization.................................................................... 112
10.6.2
About Spectral Resolution............................................................................ 112
10.7
Combinatorial Analysis................................................................................... 112
11.
Care and Maintenance .................................................................................... 115
11.1
General........................................................................................................... 115
11.2
Cleaning the Optical System .......................................................................... 115
