5
6.3.3
VIS and UV Systems with Inverted or Upright Microscope .............................54
6.4
Specimen Area .................................................................................................55
6.5
Changing Specimens........................................................................................56
6.6
Changing Objectives.........................................................................................57
6.7
Changing the Transmitted-Light Lamp Housing................................................58
6.8
Mirror housing on upright microscope...............................................................60
6.9
Changing Filter Cubes, Beam Splitters or Condenser ......................................62
6.10
Piezo focus on upright microscope ...................................................................63
6.10.1
Objective Change with Piezo Focus Configuration .........................................64
7.
Starting Up the System .....................................................................................65
7.1
Switching On the System..................................................................................65
7.2
Starting the LAS AF ..........................................................................................70
7.3
Setting Up Users...............................................................................................72
8.
Switching Off the System .................................................................................73
9.
Introduction to LAS AF .....................................................................................75
9.1
General .............................................................................................................75
9.2
Online Help .......................................................................................................75
9.2.1
Structure of the Online Help............................................................................75
9.2.2
Accessing the Online Help ..............................................................................76
9.2.3
Full-text Search with Logically Connected Search Terms...............................76
9.3
Structure of the graphical user interface ...........................................................78
9.3.1
General Structure of the Graphical User Interface..........................................78
9.4
Key Combinations.............................................................................................79
10.
Introduction to Confocal Work .........................................................................81
10.1
Preparation .......................................................................................................81
10.1.1
The Objective ..................................................................................................82
10.1.2
Conventional Microscopy ................................................................................82
10.1.3
Why Scan?......................................................................................................85
10.1.4
How Is an Optical Section Created? ...............................................................86
10.2
Acquiring Optical Sections ................................................................................88
10.2.1
Data Acquisition ..............................................................................................88
10.2.2
Illumination ......................................................................................................90
10.2.3
Beam Splitting .................................................................................................91
10.2.4
Emission Bands ..............................................................................................92
