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Switch off procedure
1)
If used, clean the oil/water immersion objective lenses with Lens Tissues
The 10x and 20x air objectives don’t need cleaning after use
2)
Leave the 10x objective selected on the microscope nose-piece to
minimise any possibility of damage during start-up when the XY motorised
stage initialises.
3)
Drop the 10x objective to the load position (lowest) via the microscope’s
focus down button or the microscope coarse focus knob
4)
Exit the Leica LAS AF software
5)
Shut Down the PicaQuant Slave PC first
Note: you can push the Slave PC mains on button quickly to shut down the PicaQuant
Slave PC if you accidently shut down the SP8 Master PC first
6)
Shut Down the SP8 Master PC
Transfer any data files to the network drives first
7)
Turn the Laser Emission key to off
There’s no argon laser to cool down and the White Light Laser (WLL) goes to standby
automatically on exiting the SP8 LSM software, so you don’t have to wait for any of the
lasers to cool before switching off
8)
Switch the Laser Power rocker switch off
9)
Switch the Scanner Power rocker switch off
10)
Switch the PC Microscope rocker switch off
Both PCs must be OFF first
11)
Switch off the distribution block on the rack (Red LED button)
Note: this controls power to the PicaQuant PC and both PC monitors
12)
Switch the epi-fluorescence Xe mercury lamp power box off
If you notice or encounter any problems when using the Leica SP8 microscope,
please write the details in the SP8 log book provided (A5 black diary).
Summary of Contents for SP8-X
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