Leica SP8-X, Abridged Instructions

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Introduction
The Leica SP8-X SMD confocal microscope is located in room 00/075, Lab 1. The
microscope is suitable for both live cell and fixed specimens on slides, and it will
run alongside the old Zeiss LMS 510 MetaHead confocal.
The new Leica SP8 has:
• An inverted DMI 6000 AFC microscope for live cell viewing
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Full live cell chamber with temperature, CO2 and humidity control
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SuperZ Galvo Stage and hardware Auto-Focus
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10x & 20x HC PL Fluotar air objectives, and 40x & 63x HC PL Apo oil CS2
objectives
• HC PL Apo x63 Mott Corr Water CS2 Objective with software control of the
correction collar
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Transmitted Light Brightfield Detector
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Inverted motorised scanning XY Stage including Mark & Find and
Autostitching/Mosaic Modules
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Passive anti-vibration microscope air-table
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AOBS filter free scanhead
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WLL2 tuneable pulsed single diode laser with 200 lines from 470 to 670nm,
including Pulse Picker for FLIM (80, 40, 20 10 MHz rep. rate)
• 405nm Diode Laser for DAPI
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Two Internal Spectral Detector Channels (PMT)
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Two Internal Spectral Detector Channels (HyD) GaAsP
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SMD Excitation: 440nm laser pulsed for FLIM
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SMD Emission FLIM+FCS 2Ch HyD Fibre coupled X1 port
• SMD Filter Cubes Alexa488/Alexa633 and CFP/YFP, others available
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Twin premium Workstations and 30 inch Monitors
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Software Modules for all Acquisition modes, 3D, FRAP/FRET/FLIP, Spectral
Dye Separation, Online Dye Separation, Live Data Mode advanced live cell
imaging module, Image Processing, Measurement & Reporting
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Extra licence for PQ ST Offline Software FCS_FLIM