Leica DM4000M Operating Manual Download

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Published September 2007 by:

Herausgegeben September 2007 von:

Leica Microsystems CMS GmbH

Ernst-Leitz-Straße

D-35578 Wetzlar (Germany)

Responsible for contents:

Verantwortlich für den Inhalt:

Dr. Jasna Roeth, Stefan Motyka

(Marketing CM, Compound Microscopy, Product Management)

(Marketing CM, Compound Microscopy, Produktmanagement)

Holger Grasse

(Safety Officer according to MPG §30)

(Sicherheitsbeauftragter nach MPG §30)

In case of questions, please contact the hotline:

Bei Fragen wenden Sie sich bitte an die Hotline:

Phone +49 (0) 64 41-29 22 86

Fax

+49 (0) 64 41-29 22 55

E-Mail: [email protected]

Summary of Contents for DM4000M

Page 1: ...1 Leica DM4000B Leica DM4000M Leica DM4500P Leica DM5000B Operating Manual Bedienungsanleitung...

Page 2: ...a Roeth Stefan Motyka Marketing CM Compound Microscopy Product Management Marketing CM Compound Microscopy Produktmanagement Holger Grasse Safety Officer according to MPG 30 Sicherheitsbeauftragter na...

Page 3: ...3 Leica DM4000B Leica DM4000M Leica DM4500P Leica DM5000B Operating Manual...

Page 4: ...emark of Microsoft Corpora tion The names of companies and products used herein may be trademarks of their respec tive owners The instructions contained in the following doc umentation reflect state o...

Page 5: ...x 1 6x tube optics 58 8 9 Light sources 59 8 10 Aperture diaphragm and field diaphragm 59 Contents 1 Important notes about this manual 7 2 Intended purpose of the microscope 8 3 Safety notes 9 3 1 Gen...

Page 6: ...erence contrast 68 9 1 5 1 DM4500 B DM4500 P 68 9 1 5 2 DM5000 B 69 9 2 Fluorescence 70 10 Contrast methods for Leica DM4000 M 71 10 1 Incident light 71 10 1 1 Bright field 71 10 1 2 Dark field 71 10...

Page 7: ...ed when operated incorrectly Explanatory note Instructions on disposing of the microscope accessory components and consumables Item not contained in all configurations Text symbols pictograms and thei...

Page 8: ...ncil Directive 98 79 EEC concerning in vitro diagnostics They also conform to the Council Directives 73 23 EEC concerning electrical ap paratus and 89 336 EEC concerning electromag netic compatibility...

Page 9: ...the user must follow the instructions and warnings contained in this operating manual Caution The devices and accessories described in this operating manual have been tested for safety and potential h...

Page 10: ...Hz max 155VA 2xT2A IEC 127 10 36 C max 80 to 30 C II 2 90 250 V 50 60 Hz max 290VA T6 3 A IEC 60127 2 3 15 35 C max 80 to 30 C II 2 3 2 Electrical safety General specifications Leica CTR5000 electroni...

Page 11: ...sation which can damage the electrical and opti cal components Operating temperature 15 35 C Caution Before exchanging the fuses or lamps be absolutely certain to switch off the main power switch and...

Page 12: ...rized shutter Manual fully encoded DM4000 B 6x 7x with M25 thread DM5000 B 7x M25 DM5000 B With object prism disk 3 positions Leica DM4000 M Leica DM4500 P Transmitted light DM4000 M BF DF PH ICT Pol...

Page 13: ...5x 2x Motorized condenser head Condenser disk for the light ring DF Stop DIC prisms Automatic K hler illumination Optional polarizer integrated and motorized Operating buttons on the stand for all mo...

Page 14: ...nt Leica DM4000 B Leica DM5000 B Only for the Leica DM5000 B Separate operating unit with a power supply for 100 W halogen lamps See p 10 Electrical safety Leica DM4000 M Leica DM4500 P Specification...

Page 15: ...ld diaphragm operating buttons 9 Transmitted light incident light switch 10 Aperture diaphragm operating buttons 11 Brightness adjustment buttons 12 Focus wheel with coarse and fine adjustment 13 Vari...

Page 16: ...Transmitted light filter optional 18 Transmitted light filter optional 19 Variable function keys preset at the factory 20 x y coaxial drive adjustable height 21 Handwheel for fine focus 22 Motorized f...

Page 17: ...ng on product con figuration The external ebq 100 supply unit is delivered in a separate package For the Leica DM5000 B microscope The Leica CTR5000 electronics box is delivered in a separate package...

Page 18: ...p climatic zones require special care in order to prevent the build up of fungus See the chapter on Care of the microscope p 77 for additional instructions Caution Electrical components must be assemb...

Page 19: ...age When using intermediate systems and optical accessories the sequence may vary In this case read chapter 6 10 Optional accessories p 33 6 1 Specimen stage Caution Never install objectives before as...

Page 20: ...et the stage clamp onto the dovetail guide 4 2 and push the stage down wards until the upper end of the dovetail guide is tightly fastened to the upper end of the stage clamp Firmly tighten the stage...

Page 21: ...r there is an orien tation pin 6 1 that must be locked into place in the guiding notch 7 1 Tighten the condenser s clamping screw 5 3 until the condenser locks into place Connect the condenser over th...

Page 22: ...9b 1 Insert the tube into the circular receptacle dovetail ring Retighten the clamping screw 9b 1 For the MBDT motorized tube only Connect the tube to the stand with the con nector bushing 10 1 Fig 9b...

Page 23: ...ransmitted light axis Caution Ensure that the lamp housing has been dis connected from the power supply Unplug the power plug and the power supply during assembly Caution Light sources pose a potentia...

Page 24: ...ew Fig 12 Lamp housing 107 2 Releasing the fastening screw 1 2 Fig 13 Lamp housing 107 2 opened 1 Mount with halogen bulb 2 Collector Fig 14 Rear view of the stand 1 Incident light lamp housing recept...

Page 25: ...the glass parts of the burner with bare hands Never look directly into the beam path blinding hazard Lamp housing 106 106 z This lamp housing is suitable for use with a 12 V 100 W halogen lamp or a va...

Page 26: ...Avoid finger prints on the lamp Remove the dust cover Insert the lamp mount with the burner in stalled into the lamp housing and tighten it with the screws 16 8 Close the lamp housing and retighten th...

Page 27: ...following gas discharge lamps may be used and require different power supplies and lamp mounts Fig 19 Type Typical bulb life 50 W high pressure mercury burner alternating current 100 hrs 100 W high pr...

Page 28: ...sing unscrew the fastening screws on the cover Remove the transport anchor red plastic rod in place of the burner in the lamp mount To do so remove the lower clamp 19 1 Pull up the cooling element 19...

Page 29: ...the side Connect the lamp housing to the external power supply 22 1 1 Fig 20 106 106 z lamp housing on the side open 1 Cover raised 2 Collector 3 12 V 100 W lamp or gas discharge lamp in mount 4 Refl...

Page 30: ...s in operation Remove the face plate from the upper part of the microscope Fig 25 Press the locking pin 25 2 to turn the turret disk When the locking pin is released the turret disk locks into place a...

Page 31: ...pensation slot Incident light polarizers R P polarizer rotating polarizer L ICR polarizer R ICR polarizer Remove the plug cap on the right side of the incident light axis Fig 29 Insert the polarizer i...

Page 32: ...light turret disk p 30 For details on the proper position for the analyzer cube please refer to the identification sheet provided Fig 30 Inserting the analyzer 1 The plug cap is replaced with the anal...

Page 33: ...mirror housing directly onto the lamp housing receptacle on the back of the stand and attach it using the side clamping screw Place the lamp housing s on the mirror hous ing and fasten it using the a...

Page 34: ...stand 1 Connecting to the CTR5000 electronics box 6 11 Connection to the power supply After completing the assembly work connect the stand to the power supply using the power cable Fig 34 2 6 12 Conn...

Page 35: ...sed on the currently activated light axis transmitted light or incident light In addition to the preset function keys for INT AP and FD there are also variable function keys Variable function keys At...

Page 36: ...eld diaphragm transmitted light SHUTTER TL Opens closes the transmitted light shutter FLUO Fluorescence last filter cube CUBE 1 Chooses the fluorescence cube on position 1 CHANGE CUBE Switches through...

Page 37: ...he fluorescence cube on position 1 CHANGE FLUO Switches through all filter cubes CONOS DM4500 P only The Bertrand lens is in the beam path conoscopic beam path FOCUS FINDER Finds the smallest incident...

Page 38: ...ecessary to correct the centering after the microscope has been trans ported and assembled Before proceeding with the necessary steps first familiarize yourself with the stand s display and control pa...

Page 39: ...ve magnification OBJ sometimes followed by the re magnification of the magnification changer appears along with the total magnification Objective x re magnification x eyepiece Light intensity The curr...

Page 40: ...anging the key as signments 7 4 The function keys There is a row of function keys both on the right and left side of the stand These can be broken down into fixed and variable buttons The vari able fu...

Page 41: ...pears in the first line of the display Note on DM4500 P Ensure that the Bertrand lens is swiveled outwards Fig 39 Stage with specimen holder 1 Object motion X direction 2 Object motion Y direction 3 S...

Page 42: ...able aperture and field diaphragm values have been preset for each objective The inci dent light module has also been centered at the factory However it may be necessary to readjust the incident light...

Page 43: ...ze of the diaphragm to the chip size of the camera Adjusting the aperture diaphragm for DM4000 M and DM4500 P only Remove one eyepiece e g right Close the aperture diaphragm with the AP function key 3...

Page 44: ...ring in the condenser disk Therefore a check must be performed for each objective When rotating in an objective that is suitable for phase contrast the corresponding light ring is set automatically P...

Page 45: ...st objectives Always remove the centering keys after the centering procedure Fig 44b Light ring centering 1 Centering key in the phase contrast centering hole 2 Polarization contrast centering hole 1...

Page 46: ...ion when the name of the exchanged filter cube is shown in the upper right of the display Caution Never look directly into the beam path Beware of the glare hazard when switching to reflector BF or Sm...

Page 47: ...he mirror image of the lamp fila ment using the adjusting knobs 46 2 and 46 4 and focus it using the reflector 46 3 Align the mirror image symmetrically to the fila ment image Fig 49 To do so again us...

Page 48: ...ering plane Fig 51 Then rotate the mirror image of the arc with the adjusting knobs 46 2 and 46 4 and fo cus it using the reflector 46 3 Orient the mirror image symmetrically to the direct image Fig 5...

Page 49: ...the direct arc image in the middle of the centering plane whereby the bright tip of the arc the focal spot should lie slightly outside of center Fig 54 Then pivot the arc s mirror image with the ad j...

Page 50: ...mirror image can no longer be su perimposed exactly In this case align both images Using the collector defocus the image with the knob 46 6 until the arc image and mirror image are no longer recogniza...

Page 51: ...itch All motorized microscope components will then run through an initialization phase After the initialization is complete the display on the stand Fig 57 shows the current micro scope setting Fig 57...

Page 52: ...0 5 1 and 2 mm Changing them requires forceful axial removal When attaching the new lock button make sure that the catch pins on the inside are properly positioned For smaller microscopes the limit s...

Page 53: ...es it possible to simultaneously grasp the coaxial drive with your hand while operating the fine adjustment with one finger 8 4 Tubes Note Close any unused tube openings as otherwise stray light can i...

Page 54: ...be The light division between the observation and documentation ports has a definite presetting 50 50 Fig 61 Individual settings of the AET22 tube BDT25 tube The light division is set manually by pull...

Page 55: ...ght into sharp focus Do not use the focus wheel 8 6 Objectives The objectives are moved into the beam path manually Be sure that the turret locks into place The positions of the objectives in the obje...

Page 56: ...ve the centering key must no longer be in the corresponding open ings When centering the objectives Fig 64 65 use two hexagon wrenches to move the objectives so that the optical axis of the objective...

Page 57: ...for clean ing or other such procedures and then rein serted into the same holes If the coarse drive or the height adjustment device is used to change the height of the specimen stage for example when...

Page 58: ...o the 1x tube factor is sufficient Setting the tubes and eyepieces p 53f Features 1x tube factor can be switched to 1 6x Bertrand lens can be activated encoded focused and centered Iris diaphragm in t...

Page 59: ...Coarse adjustment 0 255 Fine adjustment The intensity is individually adjusted and stored for each objective and contrast method For fluorescence The brightness is adjusted in 5 defined incre ments FI...

Page 60: ...TL Switch to the transmitted light axis TL by pushing the TL IL function key Select the PH phase contrast contrast meth od Do so by pressing the variable button PH Alternatively press the variable bu...

Page 61: ...y blocked for this method DF flashes in the display The microscope automatically selects the cor rect light ring in the condenser When the dark field method is selected the ap erture diaphragm is open...

Page 62: ...t of non birefringent or opaque particles then the ana lyzer is usually turned a few degrees away from the cross position to make these particles somewhat visible for specimens that remain dark when t...

Page 63: ...x i e refractive index n The quartz wedge 69 7 allows various color shifts on the polarizing microscope Fig 68 Interference color with dependence on the path difference or on the thickness and color c...

Page 64: ...an iris diaphragm to mask out the measuring points Detailed information can be found in the instruc tions for the compensator The following compensators are available Elliptical compensator based on...

Page 65: ...f these in terference images and the changes that occur in these images when using a compensator make it possible to state how many axes the crystals have uniaxial or biaxial crystals how the axes are...

Page 66: ...fixed compensators can be used in place of variable compensators for determining the opti cal character Even if only one of the optical axes is within the observer s direction of sight the optical cha...

Page 67: ...vex side to the concave side of the isogyres Biaxial negative crystals The interference bands move from the concave side to the convex side Possible faults The polarizers have been damaged discolored...

Page 68: ...and aperture and field diaphragms are as signed to the objective currently in use objec tive magnification for conoscopy is 40x 63x or 100x 9 1 5 Differential interference contrast TL 9 1 5 1 DM4500...

Page 69: ...ble key Alternatively press the variable button CHANGE TL For details on key assignments please see the identification sheet ICT appears on the display The polarizer located in the condenser and the f...

Page 70: ...entification sheet This symbol appears on the display Changing the fluorescence filter cube By pressing the variable key Cube or Cube Bring the image into focus using the focus wheel and set the brigh...

Page 71: ...eica DM4000 M 10 1 2 Dark field IL Switch to the incident light axis IL by push ing the TL IL function key Select the DF dark field contrast method by pressing the variable button DF Alternatively pre...

Page 72: ...s POL Automatic procedure The ICR filter cube is automatically brought into the beam path Manual procedure Manually push the corresponding polarizer 77 3 and the IC P analyzer 78 1 on the stand into t...

Page 73: ...o the slot on the objective turret 77 2 For fine adjustment rotate the knurled screw 77 1 on the objective prism slide 10 2 Transmitted light 10 2 1 Bright field TL Switch to the transmitted light axi...

Page 74: ...orm Service and have the supply unit fuse checked Open the shutter p 39 Check the connection to the lamp housing on the microscope Transmitted light connection Incident light fluorescence connection M...

Page 75: ...he top Make sure that the cover glass thickness is correct and that it meets the specifications on the objective Be sure that a DF objective is being used The objective aperture setting is too high ma...

Page 76: ...embedding material for transmitted light specimen is birefringent Polarizers can be damaged by strong light sources Check to see if the polarizer is dam aged discolored or heavily soiled Check to see...

Page 77: ...ion water may occur 12 2 Cleaning Caution Residual fiber and dust can create unwanted background fluorescence Cleaning coated parts Dust and loose dirt particles can be removed with a soft brush or li...

Page 78: ...t to your Leica subsidiary for repair We also advise against cleaning the inside surfaces of the eyepieces The front lenses of objectives are cleaned as described under Cleaning Glass Surfaces The upp...

Page 79: ...03 W 2 11 500 139 High pressure xenon burner 75 W 106 z lamp housing Screw cap for unused objective receptacles 020 422 570 000 Screw cap M 25 Objective turret Replacement eyecup antiglare protection...

Page 80: ...ergo tube AP Aperture diaphragm BF Bright field COMBI Combination methods CONOS Conoscopy CUBE Fluorescence cube DF Dark field incident transmitted light DIC Differential interference contrast FD Fiel...

Page 81: ...0 71 72 73 Illumination manager 12 Immersion oil 55 78 Incident light axis 12 Incident light polarizers 31 Incident light shutter 39 Incident light turret disk 30 Initialization 38 Interference contra...

Page 82: ...f Conformity Download http www light microscopy com down_ce declaration_DM4000b http www light microscopy com down_ce declaration_DM4000m http www light microscopy com down_ce declaration_DM4500p http...

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