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Instruction manual

Leica CM1510 S – Cryostat
V1.4 English – 04/2010

Always keep this manual near the instrument.
Read carefully prior to operating the instrument.

/HLFD&0 6

Cryostat

Summary of Contents for CM1510S

Page 1: ...Instruction manual Leica CM1510S Cryostat V1 4 English 04 2010 Always keep this manual near the instrument Read carefully prior to operating the instrument HLFD 0 6 Cryostat...

Page 2: ......

Page 3: ...e not to be considered as warranted characteristics of our products These are determined only by the contract provisions agreed between ourselves and our customers Leica reserves the right to change t...

Page 4: ...and installation 18 4 4 Fastening the handwheel 19 4 5 Electrical connection 20 4 6 Prior to operation 20 5 Operation 22 5 1 Control panel 22 5 2 Controller 23 5 3 Setting user definable parameters 24...

Page 5: ...39 8 1 General maintenance instructions 39 8 2 Automatic circuit breaker 40 8 3 Cleaning 41 8 3 1 Spray disinfection with Leica Cryofect 41 8 4 Removing the microtome 43 8 5 Removing the microtome ho...

Page 6: ...e it serves for quick freezing and subsequent sectioning of specimen material The instrument is not designed for unattended storage of specimen material The instrument must be operated only ac cording...

Page 7: ...ions for electrical measuring control regulating and laboratory devices For current information about applicable stan dards please refer to the CE declaration of conformity on our Internet site www le...

Page 8: ...the above will cause damage to the instrument After transporting wait at least 4 hours before switching the instrument on This waiting period is necessary to allow the compressor oil which may have be...

Page 9: ...from gilled evaporator high risk of injury from sharp edges Prior to manipulating knife and specimen when changing the specimen or during breaks always lock the handwheel and cover the cutting edge wi...

Page 10: ...nse and freeze in the cold cryostat and thus may cause malfunctions or damage Warnings maintenance Only qualified and authorized service personnel may access the internal components of the instrument...

Page 11: ...in 1 is located opposite the black dot 2 Push the metal pin outward into the recess at the handwheel if neces sary move the handwheel slightly back and forth until you feel that the locking mechanism...

Page 12: ...ruction manual V 1 4 04 2010 3 Instrument components and specifications 3 1 Overall view instrument parts Fig 3 Specimen disc Section waste tray Brush shelf Knife holder CN Knife holder base Knife hol...

Page 13: ...colli sion of the specimen with the knife edge which can cause severe dam age to both knife and specimen 3 Instrument components and specifications Thermal block optional accessory Heat extractor Quic...

Page 14: ...er draw 495 W 850 W 850 W 495 W Refrigerating output at evaporator temperature 470 W 780 W 780W 470 W of 25 C Nominal pressure 25 bar maximum value Safety factor 3 Refrigerant 215 g 5 g R404A Compress...

Page 15: ...Osram Dulux S 13 W 21 light color LUMILUX bright white Cryocabinet Dimensions Width w o handwheel 880 mm Width with handwheel 680 mm Depth 680 mm Height arm rests 1010 mm Height overall 1175 mm Weigh...

Page 16: ...quate ventilation The place of installation must meet the following requirements no direct sunlight mains power socket at a distance no greater than 2 5 m length of power cord 2 5 m no extension cords...

Page 17: ...6 38503 1 Brush shelf 14 0398 13088 1 Tool set 14 0436 43463 1 Brush fine 14 0183 28642 1 Leica brush w magnet 14 0183 40426 1 Allen key size 1 5 14 0222 10050 1 Allen key size 2 5 14 0222 04137 1 All...

Page 18: ...et Move instrument to installation site on roll ers 2 and 3 The adjustable feet 1 can be used to slightly tilt the instrument max 30 degrees To lift the instrument please use the handle straps provide...

Page 19: ...washer 3 onto screw 4 with the curved surface pointing to the right side see also Fig 6 Tighten screw 4 with the size 6 Allen key To disassemble proceed in reverse order The protective foam parts bet...

Page 20: ...r to operation Verify if mains voltage and frequency in your laboratory comply with the power rating indicated on the name plate of the instrument Place the storage shelves into the chamber Insert sec...

Page 21: ...4 6 1 Mains switch and automatic circuit breaker The automatic circuit breakers mains fuses are also used as mains switch To turn the automatic circuit breaker on flip it to the upper position To disc...

Page 22: ...from the knife The fast specimen movement towards the cut ting edge must be observed carefully to pre vent the specimen from colliding with the cut ting edge as this can cause severe damage to both th...

Page 23: ...eters can be accessed by pressing the P button Each programming field is displayed for 30 seconds for entering new parameter settings After that time the display automatically resets to actual temper...

Page 24: ...ng normal operation the actual cryochamber temperature is dis played Press once Press or to select the desired tem perature Defrost time Press once more Press or to modify the indicated value The firs...

Page 25: ...ay in dication returns to actual temperature Fig 19 To set real time press once more Press or to modify the indicated value hour Press again Press or to modify the indicated value minutes Minimum swit...

Page 26: ...the disc Place the specimen on the disc and orient Remove the cover from the quick freeze shelf Place the specimen disc with the specimen in one of the bores of the quick freeze shelf and freeze the...

Page 27: ...dle the knife holder please refer to the separate instruction manual for your knife holder Lock handwheel with handle in upper posi tion If the knife holder and a knife are in place cover the knife ed...

Page 28: ...n 4 6 knife holder CN and 1 to 2 knife holder CE are ap propriate for most applications Unlock the handwheel Rotate the handwheel cautiously and check if the specimen comes in contact with the cutting...

Page 29: ...e section thickness rotate knob 1 to a setting between 1 and 60 m from 0 10 m in 1 m steps from 10 20 m in 2 m steps from 20 60 m in 5 m steps The graduation scale on the microtome reflects the select...

Page 30: ...possible contamination ensure that the drain hose of the cry ostat is placed into an appropriate waste container at all times during operation During the automatic cryochamber defrosting the quick fre...

Page 31: ...ed 1 defrosting 2 wip ing dry 3 recooling a waiting period of approx 45 min must be observed before sectioning can be resumed If the chamber temperature exceeds 5 C the defrosting cycle is interrupted...

Page 32: ...ife back into the knife box or push the blade into the receptacle for used blades pro vided at the bottom of the dispenser Remove all specimen material from the cryochamber Remove frozen section waste...

Page 33: ...Fatty G Heart and vascular G Intestinal G Kidney G Laryngeal G Lip G Liver G Lung G Lymphoid G Muscle G Nose G Pancreatic G Prostate G Ovarian G Rectal G Skin with fat G Skin without fat G Speenal or...

Page 34: ...gh and thus warm the sections Specimen too cold Static electricity air currents Specimen not cold enough Large area specimen Anti roll plate poorly posi tioned Anti roll plate poorly aligned with knif...

Page 35: ...the specimen disc Specimen disc not clamped tightly Specimen holder ball joint not clamped Knife blade not clamped tight ly enough Section thickness too thick specimen has detached from the specimen d...

Page 36: ...tion Specimen dried up Anti roll guide too warm or incorrectly positioned Static electricity Fat on corner or edge of anti roll plate Rusty knife Static electricity or air cur rents Anti roll plate to...

Page 37: ...c circuit breaker has been activated Compressor defective Leak in the cooling system Inappropriate site conditions Ventilation grid of condenser dirty Friction between slot cover and microtome housing...

Page 38: ...ca Technical Service Fig 25 Having eliminated the possible sources of error press the thermal circuit breaker 1 to turn the instrument back on If the instrument fails to switch on call technical servi...

Page 39: ...to time above all after the instrument has been dried re peatedly in a laboratory oven apply a thin coat of cryostat oil onto clamping device T piece and clamping levers of the microtome base plate g...

Page 40: ...the circuit breaker back on flipping it to the upper position Fig 26 If the automatic circuit breakers cannot be switched back on or if they are activated several times call Leica technical service 8...

Page 41: ...ubstances for cleaning and disinfection Only use the cleaning agents specified in this instruction manual such as Leica Cryofect al cohol or common commercial disinfectants based on alcohol 8 3 1 Spra...

Page 42: ...Dispose of cloth in compliance with the applicable waste disposable regulations of your laboratory 9 Set cryochamber temperature back to the originally selected value If increased frost buildup occurs...

Page 43: ...osen screws 7 for removing the microtome Hold plug 1 of coarse feed motor at metal head and disconnect Disconnect temperature sensor 2 Switch the instrument off and dis connect the mains plug before r...

Page 44: ...he microtome in an oven Place the microtome in an oven at 40 C to 50 C for several hours After repeatedly drying the micro tome in this manner it is recom mended to relubricate the cross roller bearin...

Page 45: ...t 4 Use your right hand to bring the handwheel handle in the lowest position and keep the handle in place Push the microtome to the right with your left hand and if necessary for proper align ment of...

Page 46: ...nsert a screw driver into the slot in the fuse cap lightly press the fuse inwards then rotate 1 4 turn to the left and let go The fuse will automatically be released and can then be easily removed Rem...

Page 47: ...orescent tube down to the left and pull it out of clip 5 Hold lamp with both hands and pull it to the left out of socket 6 Do not use any lamp type other than the one inserted by the manufac turer 8 C...

Page 48: ...n be balanced For that purpose loosen screw 2 and ad just knob 1 upwards or downward Retighten screw 2 to fix knob 1 in the ap propriate position 9 Optional accessories 9 1 Heat extractors Specimen fr...

Page 49: ...ot to overtighten the screw Once the specimen is removed from the disc take the thermal block out of the cold cryochamber 9 Optional accessories 9 1 3 Thermal block The thermal block 8 facilitates the...

Page 50: ...table knife guard Glass insert 50 mm 14 0419 33816 for knife holder CN Anti roll system CN glass 50 mm 14 0419 33981 Consisting of glass insert 50 mm mtal frame CN Knife support bar for knife holder C...

Page 51: ...and glass anti roll guide Clearance angle adjustable Knife holder CE for high profile disposable blades 14 0419 33991 with lateral displacement feature and glass anti roll guide Clearance angle adjust...

Page 52: ...mm 14 0416 19275 Specimen disc 30 mm 14 0370 08587 Transfer block for specimen discs 14 0416 38207 Adapter for Miles Tissue Tek specimen discs 14 0436 26747 Heat extractor stationary for mounting on...

Page 53: ...greement The warranty terms of your Leica sales organization or the organization from which you have purchased the contractual product shall apply exclusively Technical service information If you requ...

Page 54: ...be opend nor servicing commenced until the Company or service engineer have received a satisfactory certificate Should returned goods be considered a hazard by the Company they will be returned immed...

Page 55: ...rn shipment of articles sent to Leica without prop er decontamination documentation If you have any further question please call your local Leica organisation Leica Internal Use If applicable note cor...

Page 56: ...56 Instruction manual V1 4 04 2010 12 Information for the People s Republik of China...

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