Aperio GT 450 DX User’s Guide, Revision B © Leica Biosystems Imaging, Inc. 2022
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Chapter 3: Slide Preparation
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There is no mounting adhesive around the edges of a slide
. This can cause it to stick or catch in the scanner stage
area.
CAUTION:
Do not attempt to scan damaged or broken slides, as doing so may damage the scanner.
Tissue Placement
Place the tissue in the middle of the slide, a distance from the edges of the slide, the label, and any other markings. Ensure
the following:
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The coverslip covers all the tissue.
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The label does not cover any tissue.
The example below shows minimum distances from the side and bottom edges of a 26mm x 76mm slide.
≥ 0.77 mm
≥ 0.31 mm
≥ 1.61 mm
For more details on slide specifications, see
“Slide Types Supported” on page 26
Fixing Slide Preparation Errors
Some physical problems of a slide can be resolved by cleaning the slide with a lint-free cloth or trimming the sides with a
razor blade. Permanent problems with a slide may require the preparation of a new slide.
Coverslips
Coverslips are required when using the Aperio GT 450 DX. They must be fully-cured or have completed the on-board drying
process for an automated coverslipper, such as the Leica Biosystems HistoCore SPECTRA Coverslipper.
The
Aperio GT 450 DX supports common industry glass or plastic coverslips used for 1-inch x 3-inch (2.54 cm x 7.62 cm)
slides. See the
Aperio GT 450 DX Scanner Specifications
for coverslip specifications. (Glass coverslips are recommended.)
The quality of the scan is affected by the condition of the coverslip.
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Keep the mounting media that attaches the coverslip to the slide to a minimum. Excess mounting media makes it
hard for the tissue finder to distinguish between actual tissue and the mounting media.
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Make sure there are no air pockets under the coverslip.
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For best results, do not mark or write on the coverslip.
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The coverslip must not hang over the side of the slide.
