LC Packings SWITCHOS II, User Manual

Page: 52 / 90

Software Control
4-10
D850R3
User’s Manual Switchos II
f) Define the gradient in the Flow Gradient Options box FIGURE 4-10).
FIGURE 4-10 The Flow Gradient Options Box
Note: The Flow Gradient box is not present if the ‘isocratic’ option was selected
in the Pump Options box in the previous step.
g) Setup the pressure limits for the separation column and the UltiMate pump,
and define separation column parameters (e.g. I.D., length and stationary
phase) in the
UltiMate Pump Options box (FIGURE 4-11).
FIGURE 4-11 The UltiMate Pump Options Box
Note: If you do not specify the ‘Column’ parameters (e.g. the option ‘other’ is
selected), you need to enter a CRP (Column Resistance Parameter) value. The
CRP is required to calculate the (master) flow rate of the UltiMate Micropump.
h) Specify the detector settings (e.g. the data collection rate) in the UV Options
box.
i) Define the type of injection you want to use and the corresponding injection
parameters in the
Sampler Options box.