LC Packings SWITCHOS II, User Manual

Page: 35 / 90

Installation and Getting Started
User’s Manual Switchos
D850R3
2-13
Dimension Standard Version Inert Version
50 cm x 130 µm I.D. P/N 160180 P/N 160180
100 cm x 130 µm I.D. P/N 160181 P/N 160181
b) Connect port 6 of the injection valve of the FAMOS Microautosampler to the
port 1 of valve A of the Switchos II as described in item a).
c) Connect the outlet flow of the UltiMate System (e.g. on the left side panel or
from the upper T-Piece of the flow splitter) to port 4 of valve A of the
Switchos. Use the appropriate tubing for your application.
Application (I.D.) Standard Version Inert Version
Capillary LC (50 µ m) P/N 161479 P/N 161261 (1)
Nano LC (20 µm) P/N 160178 P/N 161259 (1)
(1) use PEEK fingertight fittings only
d) Connect port 3 of valve A of the Switchos to the UltiMate column bulkhead or
directly to the micro column located in the UltiMate column compartment. Use
the appropriate tubing for your application.
Application (I.D.) Standard Version Inert Version
Capillary LC (50 µm) P/N 161480 P/N 161262 (1)
Nano LC (20 µm) P/N 160179 P/N 161260 (1)
(1) use PEEK fingertight fittings only
e) Connect ports 6 and 10 of valve A of Switchos to waste (e.g. using 200 -
500 µm I.D. PTFE tubing).
f) Connect the trap column between ports 2 and 5 of valve A of the Switchos II,
using the appropriate tubing supplied with the trap column.
A typically loading solvent is 100% water with 0.05% TFA. Fill solvent bottle A
with this solvent. Fill bottle B with either the same solvent or a mixture of
methanol/water (1:1), bottles C and D may be left empty).
After pre-concentrating the sample on the trap column, the valve A of Switchos
will be switched into position 10-1 to elute the sample (FIGURE 2-13).
Micro flow
from UltiMate
Waste
FAMOS
port 1
FAMOS
port 6
Valve A
presented
in position
10-1
Micro flow
to UltiMate
Analytical microcolumn
Trap column
1
2
3
4
5
6
7
8
9
10
Switchos
Waste
Valve A
FIGURE 2-13 Eluting Sample adsorbed on the Trap Column (in Backflush Mode)