6. Application Example
68
6.2.6
Summary of Definition of Quantitative ELISA in LT-com
1. Subtract Blank value
Definitions in LT-com
Click on
Add new transformation
in the control bar and a window appears,
asking if you want to define a
Blank reduction
. Click
Yes
and the
Blank
reduction
formula is assigned automatically to all wells.
2. Define Concentrations
Definitions in LT-com (Control bar – Method layout/ Conc.-,
Dil.-, Ref.-values)
Selected identifier: ST
Unit: UA/ml
ST1_1
5
(ST1_1 ..Standard 1 first experimental group)
ST1_2
10
(ST1_2 ..Standard 2 first experimental group)
ST1_3
20
(ST1_3 ..Standard 3 first experimental group)
ST1_4
40
(ST1_4 ..Standard 4 first experimental group)
ST1_5
80
(ST1_5 ..Standard 5 first experimental group)
ST1_6
160
(ST1_6 ..Standard 6 first experimental group)
3. Define Standard Curve
Definitions in LT-com (Control bar – Concentrations/ Standard
curve)
Input data
blank reduction
Analysis type
linear regression
X-axis
linear
Y-axis
linear
4. Define Cutoffs
Definitions in LT-com (Control bar – Evaluate data/ Cutoff
definition)
Input data:
Mean conc. (UA/ml)
Limits: 22
18
Positive
≥
22 > intermediate
≥
18 > negative
Non-competitive test
5. QC Validation
Definitions in LT-com (Control bar – Evaluate data/QC
validation):
Input data: Single conc. (UA/ml)
Validation condition 1
NC1_1<8
Validation condition 2
NC1_2<8
NC1_1 ..Negative Control first replicate first experimental group
NC1_2 ..Negative Control second replicate first experimental group
