3. Getting Started
34
3.9
Optimizing for Best Performance
The instrument has been fully factory tested to ensure that its performance is
within the specified limits (see 4.4.2 for details).
The greatest accuracy can be obtained from the instrument by observing the
recommendations mentioned below:
3.9.1
Instrument Location
The instrument should be positioned in an appropriate place (see chapter 3.3 for
detailed information).
3.9.2
Operating Procedure
General
•
It is recommended to follow the standard operating procedures for the
assays used.
•
The best reproducibility is obtained, when the measurement wavelength
corresponds to the maximum absorbance wavelength of the particular
solution.
It is important to use the maximum absorbance wavelength, if the
absorbance curve of the sample is over a narrow wavelength band.
Please be aware that measurements in the slope of an absorbance peak
will limit the accuracy of OD values
.
•
After each microplate has been measured, please refer to the test kit
package for information regarding the validation procedure.
•
Use the recommended absorbance filters for the LT-4500.
Microplates
•
The instrument can be used with those types of microplates which are
described in chapter 4.4.3. The best results are obtained when flat
bottom microplates are used. Depending on the type of microplate being
used, the measurement results may vary.
Take care especially when using microplates with C-, U- or V-
shaped bottom or strip-well plates because it is possible that the
measurement results might differ to the specifications described in
this document.
Make sure that the type of microplate used with the LT-4500
absorbance
reader is suitable for the respective application.
•
Use only perfectly clean microplates.
•
Do not allow dust to settle onto the solutions or the microplate during an
incubation period prior to the measurement.
It is recommended to use a cover for protection.
•
Inaccuracies in the amount of solution pipetted have a greater effect on
the results obtained, when small amounts of solutions are used.
•
The form of the meniscus of the solution can cause inaccuracies in the
results, particularly if small amounts of solution are used.
