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Pixel Pro 3.2

User Manual

To ensure proper use of this instrument as well as to avoid injury while operating instrument, understanding this manual completely

before use is highly recommended.

Summary of Contents for Pixel Pro 3.2

Page 1: ...R Pixel Pro 3 2 User Manual To ensure proper use of this instrument as well as to avoid injury while operating instrument understanding this manual completely before use is highly recommended...

Page 2: ...14 3 8 Histogram 16 3 9 Temperature Control only for Cooling camera 17 3 10 File Save 18 3 11 ROI 19 3 12 Mask 20 3 13 Image Stitching Live 21 3 14 EDF Live 22 3 15 Realtime Dye 23 3 16 Video Record 2...

Page 3: ...1 Automatic Counting Property 42 5 Measure 42 5 1 Measure Tool 43 5 2 Calibration 45 5 3 Measure Layer 47 5 4 Metrics Flow 48 5 5 Graphics Properties 50 5 6 Manual Class Counting 51 5 7 Scale Property...

Page 4: ...PixelPro 3 2 Instruction Manual 3 R 9 1 Preferences 62 9 2 Help 63 9 3 About 63 10 Customer Service 64...

Page 5: ...en a user combines customized configurations with customized work flows the software presents a personalized working layout ensuring accuracy for the user s experiment results whether the software is...

Page 6: ...allation automatic white balance defaults to a gamma value of 2 10 and the exposure mode to the right Industrial At software installation the default color temperature value is 6500K area white balanc...

Page 7: ...en consists of five parts status bar control bar preview window data bar picture bar Status Bar There are seven main modules in the status bar Capture Image Measure Report Camera List Display Config I...

Page 8: ...version Control Bar All function modules supported by the camera are displayed on the left side Click on a module s status bar button to check detailed functions Click the Click the button to display...

Page 9: ...ictures and videos captured or opened from other locations are displayed on the right side of the main window Thumbnails are listed in descending order by time from top to bottom Clicking any image sw...

Page 10: ...n on the left side Click the button to close the window The save location and all the images under this path will display on the right side of the window Click the button to add the current save locat...

Page 11: ...orner to exit contrast Static contrast Contrast between two saved pictures Select an image then right click the picture bar to pop up the operation menu select Contrast to open the selected picture on...

Page 12: ...icture format or picture save path Pause F7 Pause Resume the preview When the preview is paused a pause state prompt appears on the interface as shown below 3 Capture 3 1 Capture Click the button to c...

Page 13: ...he Auto Exposure box is checked the software automatically adjusts exposure time to obtain an image with appropriate brightness Area Exposure When the Area Exposure box is checked the software automat...

Page 14: ...options the area white balance box will appear for preview pictures The size of the area white balance box is adjustable Under stable lighting conditions move the area white balance box to any white p...

Page 15: ...16 bits The default bit depth of USB3 0 color camera is 8 bits The bit depth of USB2 0 camera is 8 bits only 16 bits is not supported 3 7 Image Adjust Conduct real time dynamic adjustment of pictures...

Page 16: ...pots on the microscope eyepieces and objectives With flat field calibration those types of defects can be effectively reduced imaging effects will be more uniform and color reproduction of the images...

Page 17: ...obtain more realistic images for observation and analysis Color level adjustment means redefining the R G B color levels in each channel and then re allocating pixel values Adjust color levels gradat...

Page 18: ...max 3 9 Temperature Control only for Cooling camera The software supports temperature adjustments by cooling cameras noise reduction can be achieved by reducing the working temperature of the picture...

Page 19: ...orted in the Mac version File name The file to be saved is named TS by default but the user can give the file any desired name The file name cannot contain the following special characters If the user...

Page 20: ...to change save paths The default save location is C Users Administrator Desktop Image The path cannot contain the following special characters If the user attempts to enter special characters an error...

Page 21: ...top left corner of the image as well as the width and height then click OK to apply the ROI settings 3 12 Mask Select Masking Area Use a computer mouse Click and drag within the image preview to defin...

Page 22: ...round Color The default background color of ignored areas on the stitched image is black but can be customized Click on to select the desired color in the palette The color takes effect once stitching...

Page 23: ...y images 2 Since the stitched images are large in size and will likely occupy a massive amount of computer memory once the process of stitching takes up 75 of available computer memory the stitching m...

Page 24: ...ing process A new merged Image including all depth focusing information will be generated in the image gallery 3 15 Realtime Dye This module is generally used when taking fluorograms with a monochrome...

Page 25: ...frames Total Time s The length of time spent capturing images based on how long the time is set to Setting ranges from 1 to 9999 seconds Delay time Introduces a delay before capturing images Three tim...

Page 26: ...resolution frame with the total number of frames at 10 frames and the total default capturing time at 10 seconds Image data is saved by default to the local hard drive 3 17 Delay Capture Total Time C...

Page 27: ...yback frame rate The final value of total time total number of frames playback frame rate is rounded down 3 18 Trigger only for Monochrome Cooling camera There are two output modes for image output fr...

Page 28: ...ntegration Captures continuous multi frame images according to specified parameters combines multi frame data into one image and previews integration effect in real time 1 Integration by frames combin...

Page 29: ...rames ranges from 1 to 99 Default is 10 4 Import and export are import and export correction factors respectively 5 Dark field correction should be performed for different exposure times and scenes as...

Page 30: ...currently selected parameter group files Export Saves parameter group files to a specified folder Import Loads selected parameter group files from a specified folder Reset All Restores factory default...

Page 31: ...d due to moving the sample or camera the camera will automatically adjust the focal length to ensure a clear picture One Shot AF Select focus area in preview screen Camera will conduct one time focusi...

Page 32: ...in the preview image with the mouse then double left click or double right click to complete the screenshot The screenshot will appear in the picture bar to the right Click to save the current screens...

Page 33: ...ng focal length on blurred edges of the image leading to a more vivid color in the specified area of the image Default value is 0 Adjustment ranges from 0 to 3 To finalize the parameter adjustments fo...

Page 34: ...ye Cancel Cancels adding custom colors to dyes 4 3 Fluorescence In biological related science research fields different fluorochromes are used to label different cell structures A complete fluorescent...

Page 35: ...to combine together An instructional dialog window appears as shown below 3 Select the original image requiring fluorescence synthesis from the right picture bar the synthesized image will be display...

Page 36: ...from each of multiple images captured within the same observation field but not at the same focus planes and merges them into a new image which has clearly focused areas throughout the whole image Im...

Page 37: ...alue of the pixels on a grayscale image to either 0 or 255 based on a user defined threshold This process renders the whole image in a distinct black and white format a binary image which is a digital...

Page 38: ...olor of the picture Automatic Color Scale Click Auto to set the brightest and darkest pixel in each channel as white and black respectively and then proportionally redistribute the pixel values betwee...

Page 39: ...nnel and combines complementary hues of mixed colors with the base color The combined color is always the brightest color Extract Color Extract the selected color from the RGB color group Inverse Colo...

Page 40: ...ck to enter the counting process All The counting area is the full image Rectangle Left click to diagonally opposite corners of a rectangular area The counting area is the selected rectangle Polygon L...

Page 41: ...ape of cells in the image For example if the image is of a bright cell on a dark background this function will smooth the cell boundary separate connected cells and remove small black holes in the cel...

Page 42: ...ess Next Go to next step Add Adds the selected calculation type to the statistical table in the lower pane Delete Deletes the selected calculation type from the lower pane Minimum Sets the minimum val...

Page 43: ...o 5 Precision Sets the number of significant decimal places for maximum and minimum values By default 3 decimal places are reserved Setting ranges from 0 to 6 5 Measure PixelPro provides tools for mea...

Page 44: ...ots Line Segment Draws three dots connected by line segments Third click completes the drawing Multiple Dots Line Segment Draws a line segment defined by multiple dots Single click adds a new point on...

Page 45: ...ting the two endpoints of its diameter Left click completes the drawing 3 Point Circle Draws a circle by selecting any three points on its circumference Left click completes the drawing Concentric Cir...

Page 46: ...ng data type such as length angle slope diameter perimeter area etc Graphics drawn after checking and their associated information will appear on pictures at the same time Right click to toggle betwee...

Page 47: ...on ruler The software default calibration ruler should be applied to all measurement projects Selected calibration rulers can be edited and added on the right side Right click to bring up a context me...

Page 48: ...urements and display the new measurements on Layer 01 You can choose to display the measurement data from different layers or choose to view the different layers The user can choose which measurement...

Page 49: ...gallery 2 Select the image of the standardized sample the reference image to calibrate and set the tolerances for later measurements and detections 3 Check Start Building a Metrics Flow to create a ne...

Page 50: ...the template Click Delete to delete the template if it is no longer needed 7 Select the first image to be detected and compared to the template 8 After image detection and comparison against the sele...

Page 51: ...Default value is 3 Setting ranges from 0 to 6 Line Width Sets the display line width of the measurement tool currently applied on the image Default value is 1 Setting ranges from 1 to 5 Line Style Sel...

Page 52: ...all parameters set above to measurement graphics displayed on the image Default Revert and apply the default parameter settings to measurement graphics displayed on the image 5 6 Manual Class Countin...

Page 53: ...le properties Show Scale Displays the scale on the measured image when checked Default setting is off Scale will automatically be positioned at the top left of the image and can be dragged to any desi...

Page 54: ...e font name to choose the desired font and size Border Color Sets the border color of the current scale Default color is red Click the color swatch to open the color selection palette Border Width Set...

Page 55: ...ult color is black Click the color swatch to open the color selection palette Ruler Font Sets font and size of crosshair labels No Background True is checked by default to set the label background as...

Page 56: ...Sets the line style for the grid applied to the image The default style is solid Line style options are solid line dashed line dotted line dash dot line and two dot line Line Color Sets the color for...

Page 57: ...hods Operating Procedure 1 Switch from the preview module to the measurement module or open an image and check Start to enable the function The measurement tool does not display but functions as expec...

Page 58: ...th Sets the width and height of the crosshair center currently displayed on the image Default value is 20 Color Sets the crosshair line color Click the color swatch to open the color selection palette...

Page 59: ...the software installation path Use the identifier to indicate content that needs to be displayed Where the identifier appears it means that the header of the data table is hidden Delete Deletes selec...

Page 60: ...ntifies samples or parts This custom name will show up on the working report User Name The name of the user director worker or reporter Notes Records any notes supplemental information or comments rel...

Page 61: ...m Out Reduces the current image and displays it smaller than its original size 1 1 Sets the currently displayed image to its original size Fit Adjusts the image zoom level to fit the preview window Bl...

Page 62: ...splay it in the software interface Unchecked modules will not be displayed in the interface All modules are checked by default The program remembers this setting between sessions Up Moves the selected...

Page 63: ...to the following specifications Percentage pixel Adjusts image size to the specified percentage pixel value Settings for percentage range from 1 to 200 Settings for pixel range from 4 to maximum reso...

Page 64: ...nitial selection at program start up The program must be restarted before this setting takes effect 9 2 Help View the program operating instructions for reference by the user 9 3 About Click More info...

Page 65: ...we will fulfill repairs for any damages corresponding with the warranty policies free of charge 2 The warranty policies are limited to defects in manufacturing and materials Any damage caused by the u...

Page 66: ...al 65 To save time please have the following information ready 1 Camera model and S N product serial number 2 Software version number and computer system configuration information 3 As many details as...

Page 67: ...D and Pixel Pro are registered trademarks of Labo America Inc With a policy of continuous development Labo America Inc reserves the right to change design and specifications without prior notice 2011...

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