21
Buffer Preparation - Proteins
Towbin Buffers
Native Gels
Towbin Buffer pH 8.3
25 mM TRIS, 192 mM glycine, 20% Methanol,
3.0 gm TRIS
14.4 gm glycine
200 ml Methanol
add ddi H2O to 1 liter
Denatured Gels
Towbin Buffer pH 8.3, no Methanol
25 mM TRIS, 192 mM glycine
3.0 gm TRIS
14.4 gm glycine
add ddi H2O to 1 liter
