9
Connecting the Pneumatic Pump K–1900
Setting up the K-1900
Never run the Pneumatic Pump K–1900 without liquid in the pump head or
in the piston backflushing compartment. For piston backflushing, follow
the instructions below. Operation with-out liquid may lead to damage of
the pump seals in short time.
Piston Backflushing
Backflushing the piston removes traces of salt and other decontaminants from
the backside of the pistons.
Especially if you use saline solvents or buffer solution we highly recommend
that you continuously backflush in order to prevent crystallization which can
damage your piston seals.
For best protection and error free long time operation of the pump head it is
recommended always to backflush the piston with an appropriate solvent
(mostly water) according to the following SOP.
SOP 1
Preparing your K–1900 for piston backflushing
This SOP applies to the Pneumatic Pump K–1900.
1.
Push a 1/16" ID tube over both piston backflushing capillaries.
Fig. 7
Piston backflushing capillaries
2.
Place one low end of the tubing in a flask.
3.
Fill the priming syringe with the backflushing liquid.
4.
Connect the syringe with the other tubing.
5.
Press liquid through the pump head, until it flows without any air bubbles
into the container.
6.
Remove tubings from capillary opening.
If you want operation with continuous backflushing you can attach two
containers of rinsing liquid instead of the priming syringe. The containers should
be positioned that one container is located higher than the other, thus ensuring
liquid flow through the pump head without any assistance.
When not backflushing continuously, we recommend to connect both capillary
openings with a tubing to prevent vaporization of solvents and drying out of the
piston chamber.
