Connecting the Pneumatic Pump K–1900
10
L
iq
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id
P
re
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[
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]
SOP 2
Start of Solvent Delivery
This SOP applies to the Pneumatic Pump K–1900.
1.
Screw a blind fitting to the ”Eluent outlet to column”, Pos. {2.4} in Fig. 2
and open the “De-aeration screw”, Pos. {2.3} for opening flushing valve.
2.
Connect the Luer-Lock syringe with the “De-aeration capillary”, Pos. {2.2}
in Fig. 2 with the help of a short PTFE tube (ID 1,5 mm/ OD 2,1 mm). The
pump head needs to be filled with liquid by pulling eluent into the Luer-
Lock syringe until liquid enters the syringe.
3.
Close the “De-aeration screw” and exchange the blind fitting with a
regular 1/8” fittings to connect this outlet with an injection system, a
column packing device, a chemical reactor or something else.
4.
Pull the Regulator for compressed air, see Fig. 2 for loosening up. The
pneumatic can be set in operation by turning the Regulator for
compressed air to the right (required air pressure increases). The eluent
will be transported to flushing valve.
5.
The pump should be stopped by turning the Regulator for compressed air
to the left (required air pressure decreases towards 0 bar) and any unit
(e.g. the column filling device) is connected to the solvent outlet.
Air Pressure Influences
The resulting eluent pressure increases or decreases as a function of
compressed air inlet pressure. Eluent pressure will nearly be constant, if the
compressed air inlet pressure remains constant. The
Regulator for compressed
air
can be locked by pressing it to wards the instrument.
The delivery volume depends on the required air pressure and the loss of
pressure along the column or any device that generates back pressure. You
can reach the maximum delivery volume at minimum eluent pressure below 1
bar and the maximum eluent pressure at the minimum flow rate. Fig. 8 up to
Fig. 10 show the delivery volume as a function of the eluent pressure for the
100, 250 and 1000 ml pump head, respectively. In those figures you can see
the limit values for the liquid pressure and the flow rate corresponding to Table
2, “Technical data of individual pump heads,” on page 6 and the maximum
allowed air pressure for each pump head. By the constant required air pressure
the flow rate increases if the liquid pressure decreases. The Figures should help
to choose the pump head for your application.
1200
800
400
0
Required Compressed Air
4,2 bar
4 bar
100 ml
3 bar
2 bar
1 bar
0
100
200
300
Flow Rate [ml/min]
Fig. 8
Interdependence of delivery volume and eluent pressure for the 100 ml pump
head
