14
Intended use
Backflushing of a precolumn
Basics
The precolumn is used in chromatography, for example in the
following cases:
Preseparation or separation of heavily retarding substances
Protection of the main column
Note:
Precolumn and main column are aligned in a row
behind each other.
Fig. 10
Backflushing precolumn – injection from the 6P/3C valve
on the precolumn
From the injection valve, the precolumn is filled with sample
through the channel
2->3
. From the channel
6->1
, the quickly
dissolved substances on the column are measured, the heavily
retarding substances remain on the precolumn.
Note:
The eluent flushes the precolumn after switchover
in opposite direction.
Fig. 11
Backflushing precolumn – switchover of the 6P/3C valve
After switching over the valve to the channel
2->1
, the late elut-
ing substances are flushed back in opposite direction from the
precolumn to the detector.
Injection
on precolumn
1
2
3
4
5
6
Column
Precolumn
Injection
Detector
Switchover
of the direction
of flow
1
2
3
4
5
6
Column
Precolumn
Injection
Detector
