13
Intended use
Backflushing of a column
Basics
The backflushing of a column is used in chromatography,
for example in the following cases:
Analysis of heavily retarding substances
Optimization of analysis times
Flushing of a column
Note:
The eluent flushes the column after switchover in
opposite direction (reduction of the band broadening).
From the injection valve, the column is filled with sample
through the channel
2->3
. The quicker part of the substances
is separated through the channels
6->1
and
5->4
and flushed
to the detector.
Fig. 8
Backflushing – injection from the 6P/3C valve on
the column
Note:
Make sure there is no pressure when switching
between the columns so that the columns are not
damaged.
After switching over the valve to the channel
2->1
, heavily
retarding substances are eluted in opposite direction fluid from
the column and flushed back to the detector.
Fig. 9
Backflushing – switchover of the 6P/3C valve
Injection
1
2
3
4
5
6
Column
Injection
Detector
Switchover
of the direction
of flow
1
2
3
4
5
6
Column
Injection
Detector
