34
Optimizing the detector
AZURA® Detector DAD 6.1L, DAD 2.1L, MWD 2.1L Instructions, V6700
6. Optimizing the detector
In this chapter, you find information on how to optimize your detector.
6.1 General
Performance
The performance of the detector is largely dependent on the perfor-
mance of the HPLC system.
Noise can be related to pump stability, the flow cell cleanliness, lamp
quality, mobile phase composition and other factors.
Drift is usually related to long-term changes in the environment, such
as detector warm-up or fluctuations in temperature and mobile phase
composition.
Location
In order to ensure thermo stability and to prevent drift effects, note the
aspects listed in the section „4.2 Ambient conditions“ on page 11.
Warmup time
The recommended warmup time for the detectors is 30 minutes. This re-
commendation is applicable for the detector on as well as for the lamp/s.
Fig. 24:
Cold start temperature profile
6.2 Selecting the flow cell
Several different flow cells are available for the detector. Note that the de-
tector is shipped with a test cell. A flow cell must be ordered separately.
Signal sensitivity, peak broadening and response can all be affected
by the choice of flow cell. Volume, path length as well as wetted parts,
requi red pressure range, flow cell connection, and remote operation are
further factors that need considering when selecting a flow cell.
Flow cell volume
Depending on your instrument setup, column and sample(s), one flow
cell volume may be more appropriate that another. If the volume is too
large, two adjacent peaks may get mixed in the cell. If the volume is too
