13
6-4. Setting the sampling amount
Put the sample in the sample cell selected in section 6-1 and measure the absorbance to determine
the sampling amount.
* If the sampling amount (sample height) is not consistent, it may affect the absorbance.
* When not using the light shielding cover, determine the sampling amount under the same condition of
external light as measurement.
* Use the same amount in making calibration curve and measurement.
①
Set the absorbance measurement mode.
Enter the absorbance measurement mode by pressing
the [MODE] key. (→ 8. Measurement (P.16))
Immediately before performing this operation, it is
recommended to perform zero-adjustment. (→ 9. Zero-
adjustment (P.18))
②
Measure the absorbance.
Use the sample cell selected in section 6-1. Set the
sample to cover up the measurement window and
measure the absorbance.
③
Observe the change of absorbance.
As the sample is gradually raised, the fluctuation of displayed absorbance, X1 to X6, will become stable
at a point where the near-infrared pass-through is stopped. Consider the sampling amount at this point
as "absorbance saturated sampling amount". If the sampling amount is sufficiently prepared, set the
sampling amount more than the "absorbance saturated sampling amount".
* Refer to P.30 <Remarks>, if the absorbance saturated sampling amount cannot be prepared.
0 1
X 1
0 . 0 0 0 0
X 2
− 0 . 0 0 0 2
X 3
0 . 0 0 0 1
X 4
0 . 0 0 0 2
X 5
− 0 . 0 0 0 1
X 6
0 . 0 0 0 3
0 1
X 1
0 . 1 9 2 7
X 2
0 . 0 2 0 1
X 3
0 . 2 8 0 4
X 4
0 . 1 9 2 6
X 5
0 . 2 6 2 2
X 6
0 . 3 1 2 0
Sampling
amount
Sampling
amount
Sampling
amount
Near-infrared
Near-infrared
Sampling
amount
Absorbance
While the near-infrared from the bottom
passes through the sample, the displayed
value will continue to fluctuate.
Ex.)
Ex.)
When the sample height is raised, there will be a point
where the near-infrared pass-through is stopped.
Then, the value will become stable.
Consider the point
where only the last 2
digits of absorbance
f l u c t u a t e a s t h e
absorbance saturated
sampling amount.
X 1
0 . 2 9
8 5 . . . . .
0 . 2 9
9 4
X 2
0 . 0 3
5 7 . . . . .
0 . 0 3
6 2
X 3
0 . 3 9
8 2 . . . . .
0 . 4 0
0 6
X 4
0 . 2 8
8 7 . . . . .
0 . 2 9
0 7
X 5
0 . 3 7
5 2 . . . . .
0 . 3 7
7 4
X 6
0 . 4 3
2 0 . . . . .
0 . 4 3
4 9
No more change in raising the height of sample
X 1 0 . 1 9 2 7 . . . . . 0 . 2 6 9 5 . . . . . 0 . 2 8 9 7
X 2 0 . 0 2 0 1 . . . . . 0 . 0 2 8 6 . . . . . 0 . 0 3 3 4
X 3 0 . 2 8 0 4 . . . . . 0 . 3 6 8 7 . . . . . 0 . 3 9 1 4
X 4 0 . 1 9 2 6 . . . . . 0 . 2 6 1 4 . . . . . 0 . 2 8 2 0
X 5 0 . 2 6 2 2 . . . . . 0 . 3 4 6 0 . . . . . 0 . 3 6 8 5
X 6 0 . 3 1 2 0 . . . . . 0 . 4 0 2 1 . . . . . 0 . 4 2 5 0
Ex.)
