5
Contents
SECTION 1 – Introduction ....................................................................................... 8
1.1
INSTRUMENT DESCRIPTION............................................................................................. 8
1.2
INSTRUMENT SPECIFICATION ......................................................................................... 8
SECTION 2 – Installation ......................................................................................... 9
2.1
UNPACKING ........................................................................................................................ 9
2.2
INSTALLATION .................................................................................................................... 9
2.3
DISPLAY ............................................................................................................................. 10
2.4
CONTROLS ........................................................................................................................ 11
2.5
REAR PANEL ..................................................................................................................... 13
2.6
FRONT VIEW ..................................................................................................................... 13
SECTION 3 –
THEORY AND PRACTICE OF SPECTROSCOPY MEASUREMENTS
..... 14
3.1
THEORY OF SPECTROSCOPY MEASUREMENT .......................................................... 14
3.2
NUCLEIC ACID DETERMINATION ................................................................................... 15
3.3
SPECTROSCOPY MEASUREMENT ................................................................................. 15
3.4
GOOD PRACTICE GUIDELINES ....................................................................................... 16
SECTION 4 – INSTRUMENT SETUP ...................................................................... 18
4.1
INSTRUMENT NAVIGATION ............................................................................................. 18
4.2
MAIN MENU SCREEN ....................................................................................................... 18
4.2.1
Slit Width Setting ................................................................................................................ 18
4.3
SYSTEM UTILITY MENU ................................................................................................... 19
4.3.1
Wavelength Reset .............................................................................................................. 19
4.3.2
Printer Setup ....................................................................................................................... 19
4.3.3
Lamp Setup ........................................................................................................................ 20
4.3.3.1
Change the Lamp Switch Point .................................................................................. 20
4.3.4
Clock Setup ........................................................................................................................ 20
4.3.4.1
Set Time ..................................................................................................................... 20
4.3.4.2
Set Date ..................................................................................................................... 21
4.3.4.3
Display Time / Date .................................................................................................... 21
4.3.5
Refresh Dark Current .......................................................................................................... 21
4.3.6
Connect to PC .................................................................................................................... 21
4.3.7
Beeper On/Off ..................................................................................................................... 21
4.3.8
Language Selection ............................................................................................................ 21
4.3.9
Refresh System Baseline ................................................................................................... 21
4.3.10 Delete Entire Saved Files ................................................................................................... 22
4.3.11 Restore Default Settings ..................................................................................................... 22
SECTION 5 – PHOTOMETRICS.............................................................................. 23
5.1
PHOTOMETRICS MENU SCREEN ................................................................................... 23
5.2
METHOD SET UP .............................................................................................................. 23
5.2.1
Selecting a Wavelength ...................................................................................................... 23
5.2.2
Selecting the Measurement Mode ...................................................................................... 23
5.2.3
Selecting the Unit of Measurement .................................................................................... 24
5.2.4
Entering a Concentration Factor ......................................................................................... 24
5.3
CALIBRATION .................................................................................................................... 24
5.3.1
Zero Calibration .................................................................................................................. 24
5.3.2
Calibrating to a Standard .................................................................................................... 25
5.4
SAMPLE MEASUREMENT ................................................................................................ 25
