42
Absorbance Difference 2
Purity Ratio = Abs@260nm / Abs@230nm
RNA Conc ( g/ml) = (Abs@
260
x 49.1) – (Abs@
230
x 3.48)
Protein Conc ( g/ml) = (Abs@230 x 183) – (Abs@260 x
75.8)
The option to include the third wavelength as a reference is given after the measurement mode is
selected. Use the up and down arrow keys to select Yes or No and press the Enter key to confirm.
Selecting the Yes option will result in all the absorbance value measured at wavelength 3 being
subtracted from the photometric values measured at wavelengths 1 and 2.
9.3
WAVELENGTH SELECTION
The measurement wavelengths of the selected measurement mode can be adjusted by pressing the
GOTO key on the instrument keypad. The required wavelengths can then be entered using the
numerical keypad. Press the Enter key to confirm.
9.4
ADJUSTING THE CONCENTRATION CALCULATION FACTORS
The measurement mode option can be selected by
pressing the function key below the Coeff option.
The new concentration calculation factors can be
entered using the numeric keypad. Press the Enter key
to confirm.
9.5
SELECTING THE UNIT OF MEASUREMENT
The unit of measurement can be selected by pressing the function key below the Unit option. The
required unit of measurement can then be selected from the available options of IU, mM/L, M/L,
g/mL, mg/mL, mg/L, mEq, ppb, ppm, % and other. Press the Enter key to confirm.
