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5.4.3 Measuring a Sample after Calibrating to a Standard

Remove the cuvette containing the standard sample

and place a cuvette containing the sample to be

measured in the sample chamber. Close the instrument

lid and the calculated concentration value will be shown

on the screen.

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Summary of Contents for 6850

Page 1: ...Operating Manual 685 030 REV A 03 12 6850 Spectrophotometer...

Page 2: ...ed to the appropriate servicing authority Merci de lire attentivement ces informations avant d installer ou d utiliser cet appareil 1 L appareil d crit dans ce manuel est con u pour tre utilis uniquem...

Page 3: ...n Umgang mit Chemikalien sollten eingesetzt werden 5 Besteht der Verdacht dass die Sicherheitsvorrichtungen in irgendeiner Weise besch digt wurden muss das Ger t au er Betrieb genommen und gegen weite...

Page 4: ...operarios como el personal de servicio utilicen un sistema de trabajo seguro as como las instrucciones detalladas que se especifican en este manual 3 Cualquier elemento que no se encuentre entre los...

Page 5: ...MENU 19 4 3 1 Wavelength Reset 19 4 3 2 Printer Setup 19 4 3 3 Lamp Setup 20 4 3 3 1 Change the Lamp Switch Point 20 4 3 4 Clock Setup 20 4 3 4 1 Set Time 20 4 3 4 2 Set Date 21 4 3 4 3 Display Time D...

Page 6: ...S 32 SECTION 7 SPECTRUM 33 7 1 SPECTRUM MODE SCREEN 33 7 2 METHOD SETUP 33 7 3 SELECTING THE MEASUREMENT MODE 34 7 4 SAMPLE MEASUREMENTS 34 7 5 ADJUSTING THE DISPLAYED SCAN RANGE 34 7 6 SPECTRUM SEARC...

Page 7: ...E PASSIVE AND ACTIVE ACCESSORIES 47 11 2 1 Passive Accessories 47 11 2 2 Active Accessories 48 11 2 2 1 8 Position Automatic Cell Changer 48 11 3 USING THE 8 POSITON AUTOMATIC CELL CHANGER 49 11 4 SPA...

Page 8: ...and 5nm Photometrics Transmittance 0 to 200 Absorbance 0 3 to 3 0A Accuracy 0 3 T 0 100 T 0 002A 0 0 5A Reproducibility 0 001 Abs 0 to 0 5 Abs 0 002 Abs 0 5 to 1 0 Abs Resolution 0 1 T 0 001A Stray l...

Page 9: ...35 028 6 Dust Cover 685 040 7 PC software CD and USB security dongle 685 035 2 2 INSTALLATION The 6850 is supplied ready to use The working temperature and humidity range of the 6850 spectrophotometer...

Page 10: ...The instrument has an inclusive display which enables text and graphs to be displayed clearly Following successful completion of the power on tests and the instrument warm up time the main menu scree...

Page 11: ...ment modes entering numbers saving and analysing results The keypad is displayed below Fig 2 4 1 Keypad Function Keys Allows on screen options to be selected Numeric Keys Enter numbers and letters Sel...

Page 12: ...ement GOTO Key Set the instrument wavelength using the numeric keys Press the Enter key to confirm PRINT Key Print result ENTER Key Confirm operation CELL Key Select Deselect Auto cell Holder position...

Page 13: ...3 Power in socket Connection socket for power supply unit 4 LCD contrast adjustment Connection to a PC or external serial printer 5 Parallel port Allows the accessory printer to be connected 6 USB por...

Page 14: ...is travelling through The Beer Lambert law states that A is the absorbance is the molar absorption coefficient l mol 1 cm 1 c is the concentration mol l 1 l is the path length cm This law shows that...

Page 15: ...bs 260nm x 49 1 Abs 230nm x 3 48 Referring to a blank value where no absorption should occur is commonly required On the 6850 the default reference wavelength is 320nm and the user can include the mea...

Page 16: ...uvettes must be used b Plastic disposable cuvettes should only be used ONCE c Glass cuvettes should be thoroughly cleaned after use Discard when scratches become evident on optical surfaces d Care sho...

Page 17: ...for dissolving or diluting samples chemicals and reagents 8 All measurements require calibration to a blank for maximum accuracy this should be prepared with care using the same deionised water or sol...

Page 18: ...measurement modes the system utility menu and the variable slit width setting The available measurement modes are photometrics quantitation spectrum scan kinetics DNA Protein and multi wavelength The...

Page 19: ...aseline measurement 10 Perform an instrument reset 4 3 1 Wavelength Reset Highlight the wavelength reset option and press the Enter key to confirm This will recalibrate the wavelength setting of the i...

Page 20: ...where the instrument will switch between the deuterium and tungsten lamps Enter a wavelength between 300 and 360 nm and press the Enter key to confirm Once complete the instrument will return to the L...

Page 21: ...rs dark current values 4 3 6 Connect to PC Connect the interface cable to the USB type B port on the rear of the instrument and connect to a USB port on the PC The connecting COM port should be config...

Page 22: ...ll need to confirm this command again by selecting the Yes option with the up and down arrow keys This action will delete all saved results and methods from the instrument 4 3 11 Restore Default Setti...

Page 23: ...ration factor and the calibration standard s concentration These options are selected with the corresponding function buttons 5 2 METHOD SET UP The photometrics measurement mode is very simple and the...

Page 24: ...termined by using an appropriate multiplication factor with the measured photometric reading A concentration factor can be entered by pressing the function button below the Factor option The required...

Page 25: ...ctor is then updated on the screen 5 4 SAMPLE MEASUREMENT 5 4 1 Photometric Measurements Insert the cuvette containing the blank solution into the reference position in the sample chamber and insert t...

Page 26: ...ating to a Standard Remove the cuvette containing the standard sample and place a cuvette containing the sample to be measured in the sample chamber Close the instrument lid and the calculated concent...

Page 27: ...changed The two options at the bottom of the screen allow the user to select the unit of measurement and edit the calibration standard settings These options are selected with the corresponding functi...

Page 28: ...ow the Curve Fit option in the quantitation mode screen Four new options are available in the quantitation table screen 4 Method Change the curve fit algorithm 5 Params Enter linear regression constan...

Page 29: ...nter key to confirm 6 3 2 3 Edit Calibration Table The user can edit the calibration curve data by pressing the function key below the Standard option The user will be prompted to enter the concentrat...

Page 30: ...ution into the sample position Close the instrument lid and press the start stop button Once the measurement is complete the instrument will ask for the second standard solution to be inserted Repeat...

Page 31: ...in the same manner Once the filename has been entered press the Enter key to confirm 6 6 RECALLING A STORED STANDARD CURVE A standard curve can be opened from the instrument s internal memory to allow...

Page 32: ...reen press the Save key on the instrument keypad The instrument will ask the user to enter a filename max 8 characters using the alpha numeric keypad The user can select the indicated letters numbers...

Page 33: ...tions at the bottom of the screen allow the user to edit the scan settings select the measurement mode perform a post measurement search of the spectrum or apply a smoothing algorithm to the displayed...

Page 34: ...measurement is complete the measured spectrum scan will be displayed on the screen 7 5 ADJUSTING THE DISPLAYED SCAN RANGE The instrument will display the full range scan after a measurement is comple...

Page 35: ...lue can be entered using the numeric keypad Press the Enter key to confirm 7 7 SPECTRUM SMOOTHING The measured spectrum scan can be smoothed by pressing the function key below the Smooth option 7 8 ST...

Page 36: ...e opened from the instrument s internal memory Press the Open key on the instrument keypad The user can select from the list of stored files by pressing the up and down arrow keys Once the required fi...

Page 37: ...ions at the bottom of the screen allow the user to edit the kinetics run settings select the measurement mode perform a linear regression analysis on the data and perform a post measurement search of...

Page 38: ...e keypad Once the measurement is complete the measured kinetics scan will be displayed on the screen 8 5 ADJUSTING THE DISPLAYED SCAN RANGE The instrument will display the full range scan after a meas...

Page 39: ...and a Factor pressing the Enter key to confirm each value When all values have been confirmed the instrument will calculate the concentration value according to the following equation Conc I U Abs st...

Page 40: ...atedly pressing the key until the required value is displayed after a short period the next character can be entered in the same manner Once the filename has been entered press the Enter key to confir...

Page 41: ...select the measurement mode select the units of the measurement and reset the modes settings to their default values These options are selected with the corresponding function buttons 9 2 ADJUSTING T...

Page 42: ...hs of the selected measurement mode can be adjusted by pressing the GOTO key on the instrument keypad The required wavelengths can then be entered using the numerical keypad Press the Enter key to con...

Page 43: ...etermined results will be displayed in the DNA Protein mode screen Further samples can be measured in a similar manner 9 8 STORING METHODS RESULTS A method result file can be saved to the instrument s...

Page 44: ...es by pressing the up and down arrow keys Once the required file has been located press the Enter key to confirm If more than one result is stored in a file the up and down arrow keys can be pressed t...

Page 45: ...the user to edit the measurement wavelengths and change the measurement mode These options are selected with the corresponding function buttons 10 2 WAVELENGTH SELECTION The measurement wavelengths o...

Page 46: ...ated letters numbers and symbols by repeatedly pressing the key until the required value is displayed after a short period the next character can be entered in the same manner Once the filename has be...

Page 47: ...SSIVE AND ACTIVE ACCESSORIES There are two types of accessories which can be fitted in the sample chamber passive or active accessories The range of passive accessories includes 10 x 10mm cuvette hold...

Page 48: ...to undo the passive accessory Lift out the passive accessory On the underside of the 8 cell accessory locate the accessories power supply and communication connector Take the 8 cell accessory and ali...

Page 49: ...nd corner of the screen To activate the accessory press the Cell key on the instrument keypad The indicated cell position will be highlighted on screen Each cell position is selected by pressing the c...

Page 50: ...50 11 4 SPARES Part Code Description of Spare Part 685 020 Tungsten lamp 685 021 Deuterium lamp...

Page 51: ...se the unit should be disconnected from the mains supply and covered with the optional dust cover 12 2 LAMP REPLACEMENT Warning HOT Switch off the mains power supply and wait at least 20 minutes befor...

Page 52: ...ometer 12 2 2 Deuterium Lamp Replacement 1 Unscrew the 4 screws on the spectrophotometer s top case see 12 2 1 and remove the cover of the spectrophotometer 2 Unscrew the 2 screws on the top of the la...

Page 53: ...ce Department for a reference number which you should include with your faulty equipment Please also ensure you include a clear description of the fault and a completed copy of our Decontamination Cer...

Page 54: ...ight is not transmitted through the sample Ensure the lamp is working 13 2 TECHNICAL SUPPORT Jenway have a dedicated Technical Support team made up of experienced scientists who are on hand to help wi...

Page 55: ...55 SECTION 14 DECLARATION OF CONFORMITY...

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