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SECTION 1 - Introduction 

1.1 

INSTRUMENT DESCRIPTION 

The  6305  spectrophotometer  is  suited  to  a  wide  range  of  applications  in  education,  quality  control, 

environmental  and  clinical  analysis.  This  model  is  a  UV/visible  spectrophotometer  covering  a 

wavelength  range  from  198nm  to  1000nm,  with  measurement  modes  for  absorbance,  % 

transmittance  and  concentration.  It  has  full  interfacing  capability  for  Analogue  output  and  serial 

(RS232)  interfacing.  The  optical  system  is  independently  housed  and  isolated  with  lenses  to  give 

maximum  protection  from  environmental  contamination.  Combined  with  a  mechanically  rigid 

structure, this model provides a system with fast warm-up, low drift and high reliability.  

1.2 

INSTRUMENT SPECIFICATION 

 

6305 

Wavelength

 

Range 

198 to 1000nm 

Resolution 

1nm 

Accuracy 

± 2nm 

Spectral bandwidth 

8nm, 6nm over the UV range 

Photometrics

 

Transmittance 

0 to 199.9% 

Absorbance 

-0.300 to 1.999A 

Accuracy 

±1%T, ±0.01Abs at 1.000 Absorbance 

Resolution 

0.1%T, 0.001A 

Stability 

1%/hr after 20 minute warm up 

Concentration

 

Range 

-300 to 1999 

Resolution 

1/0.1 

Calibration 

Blank with a single standard or factor 

Units 

no units, %, ppm, M, g/l, mg/l 

Factor 

0.1 to 9999 

Standard 

0.1 to 1999 

Other

 

Beam height 

15mm 

Light source 

Xenon flash lamp module 

Outputs 

Analogue, RS232 

Power 

<50W 

Size (w x d x h) 

365 x 272 x 160mm 

Weight 

6kg 

Summary of Contents for 6305

Page 1: ...Operating Manual 635 015 REV B 06 14 Model 6305 Spectrophotometer...

Page 2: ...2...

Page 3: ...val of covers and attempted adjustment or service by unqualified personnel will invalidate any warranty and incur additional charges for repair 7 Reference should always be made to the Health and Safe...

Page 4: ...n de ces produits chimiques doivent tre respect s soigneusement Lorsqu elles ne sont pas disponibles ces donn es doivent tre demand es dans tous les cas au fournisseur avant de poursuivre 8 Il est imp...

Page 5: ...eine geeignete Sicherheitsausr stung und pers nliche Schutzausr stung zu verwenden 9 Falls vermutet wird dass der Sicherheitsschutz auf irgendeine Weise beeintr chtigt wurde ist das Spektrophotometer...

Page 6: ...are o utilizzare lo strumento 1 Gli spettrofotometri della serie 63 sono progettati per essere utilizzati da personale qualificato che conosce i principi e le applicazioni ad essi collegati Per ulteri...

Page 7: ...tiche di laboratorio quando si manipolano campioni sostanze chimiche reagenti e apparecchiature accessorie per eseguire misurazioni e analisi con questo strumento Attrezzature idonee per la protezione...

Page 8: ...RICS MEASUREMENTS 18 4 1 TRANSMISSION MEASUREMENT MODE 18 4 2 ABSORBANCE MEASUREMENT MODE 18 SECTION 5 CONCENTRATION MEASUREMENTS 20 5 1 USING A KNOWN STANDARD 20 5 2 USING A KNOWN FACTOR 20 SECTION 6...

Page 9: ...ontamination Combined with a mechanically rigid structure this model provides a system with fast warm up low drift and high reliability 1 2 INSTRUMENT SPECIFICATION 6305 Wavelength Range 198 to 1000nm...

Page 10: ...he mains fuse is housed within the POWER IN socket When replacing the fuse the unit must be disconnected from the mains supply The fuse rating is 2A F fast blow type In the event of the fuse failing a...

Page 11: ...g 2 3 1 Display 1 Primary display area Transmission Absorbance Concentration 2 Primary display adjust annunciator 3 Secondary display area Wavelength Factor 4 Primary display units 5 Secondary display...

Page 12: ...u option 4 CAL Key Initiates a calibration routine 5 Print Key Provides a printout of the current reading with an incremental sample number When pressed for the first time after a calibration the prin...

Page 13: ...ess panel and all ventilation slots must NOT be covered or obstructed at any time 2 Power switch On off switch for the unit 3 Power in socket IEC type connection socket for mains cable 4 Output socket...

Page 14: ...is travelling through The Beer Lambert law states that A is the absorbance is the molar absorption coefficient l mol 1 cm 1 c is the concentration mol l 1 l is the path length cm This law shows that...

Page 15: ...enon lamp is focused onto the grating with 1200 lines per millimeter which separates the light into discreet wavelengths The diffracted spectrum of light then passes through a further slit and lens ar...

Page 16: ...to 260nm Below this level quartz cuvettes must be used NOTE the plastic cuvettes supplied with the unit are not suitable for use below a wavelength of 320nm b Plastic disposable cuvettes should only b...

Page 17: ...In the preparation of samples and standards high grade borosilicate glass and AR grade chemicals and reagents must be used Good quality deionised water or other suitable solvents must be used for diss...

Page 18: ...screen Subsequent samples can be measured in the same way If the wavelength is adjusted between sample measurements then the instrument must be calibrated again before more samples can be measured 4 2...

Page 19: ...19 measured...

Page 20: ...ously Use the arrow keys to change the concentration to the required value for the standard Press the CAL or RETURN key to calibrate the instrument to the standard concentration The instrument will ca...

Page 21: ...also be sent to the 6305 via the serial interface using Windows HyperTerminal for example ASCII D or d Same as pressing the PRINT key ASCII T CR Outputs transmission and wavelength separated by an AS...

Page 22: ...readily incorporated into most spreadsheet software packages 6 1 RS232 Output The bi directional RS232 interface is available on the rear panel 25 way D type connector The connections are as follows T...

Page 23: ...PC GND 7 5 GND Note the interface cable kit 542 009 can be used to implement the above interconnections 6 2 Recorder Output This is available via the 4mm rear panel sockets The level is proportional...

Page 24: ...or use with computer without a serial port 543 001 40 Column printer supplied with interface cable paper roll and ribbon and universal power supply 060 287 Paper roll for printer quantity 1 060 288 Pr...

Page 25: ...or commercial use and will give rise to errors if used 8 2 LAMP REPLACEMENT 8 2 1 Xenon Flash Lamp Module Replacement Before replacing the lamp disconnect the unit from the mains supply Access to the...

Page 26: ...26...

Page 27: ...e Department for a reference number which you should include with your faulty equipment Please also ensure you include a clear description of the fault and a completed copy of our Decontamination Cert...

Page 28: ...is outside the instrument range Recalibrate the instrument using a standard of lower concentration Err 5 Lamp Failure The instrument is unable to detect a peak signal for wavelength calibration Possi...

Page 29: ...29 SECTION 10 Declaration of Conformity...

Page 30: ...748 Fax 33 1 64 45 13 00 Fax 44 0 1785 810405 e mail info bibby scientific com e mail bsf bibby scientific fr e mail bibby bibby scientificasia com www bibby scientific com www bibby scientific com ww...

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