Invitrogen EVOS XL User Manual Download Page 7

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EVOS

™

XL Imaging System User Guide

Instrument exterior components and mechanical controls

Power switch

Objective selection wheel

Power input jack

Focus knobs

USB and DVI ports

Stage Y-axis knob

Coarse stage positioning knobs

Phase annuli selector

Stage X-axis knob

Condenser slider slot

Summary of Contents for EVOS XL

Page 1: ...For Research Use Only Not for use in diagnostic procedures EVOS XL Imaging System For Transmitted Light Applications Catalog Number AME3300 Publication NumberMAN0007987 Revision A 0...

Page 2: ...ng legal regulatorylanguage reorganizecontent 1 0 8 April 2013 Basis for thisrevision Important Licensing Information These productsmay be covered by one or more LimitedUse Label Licenses By use ofthe...

Page 3: ...8 Connect power supply 8 USB and DVI output ports 8 Condenser shield 9 Install the EVOS XL Imaging System in a cell culture hood 10 3 Basic operation 11 4 Advanced operation 14 Manage users 14 Save i...

Page 4: ...n controls 38 Mechanical controls 42 Appendix D Safety 44 Safety conventions used in this document 45 Symbols on instruments 46 Safety labels on instruments 48 General instrument safety 49 Chemical sa...

Page 5: ...nstrument operation accurate installation or safe use of a chemical CAUTION Indicates a potentially hazardous situation that if not avoided may result in minor or moderate injury It may also be used t...

Page 6: ...all parts are present Contact your distributor if anything is missing Damage claims must be filed with the carrier the warranty does not cover in transit damage Note If you do not have your distribut...

Page 7: ...nt exterior components and mechanical controls Power switch Objective selection wheel Power input jack Focus knobs USB and DVI ports Stage Y axis knob Coarse stage positioning knobs Phase annuli selec...

Page 8: ...oolbar options Light ON OFF button Settings control button Illumination slider Save image button Capture image button QuickSave option Color adjustment button Selected objective Note See Appendix C Co...

Page 9: ...ld not be subjected to UV sterilization UV degrades many materials including plastic Damage from UV exposure is not covered under the manufacturer s warranty Transport the EVOS XL Imaging System to th...

Page 10: ...r jack on the right side of the instrument base attach the cord to the adapter and plug the cord into an outlet IMPORTANT Always use the correct power supply The power adapter specifications appear on...

Page 11: ...EVOS XL Imaging System User Guide 9 Condenser shield This shield can help reduce the effects that overhead lighting might have on your image...

Page 12: ...strument Note See the illustrations on page 7 for details about moving the EVOS XL Imaging System 1 Secure the stage with the stage lock pin switch the EVOS XL Imaging System off and disconnect the po...

Page 13: ...gin button displays the current user ID See Instrument exterior components and mechanical controls page 5 as needed See also Appendix C Controls glossary page 38 for more details 1 Turn on the instrum...

Page 14: ...ation using the Light ON OFF toggle in the left control bar 8 Focus the sample using the focus knobs 9 Adjust the illumination intensity if necessary using the Illumination slider on the left control...

Page 15: ...er the file name click in the Save File Name field The virtual keyboard opens 13 Enter the file name then click the Accept button 14 Choose the file type and click the Save button Note See Save images...

Page 16: ...ns Select the desired user profile then click OK Note No password is necessary to log in Add or remove a user profile 1 Click the Login button 2 To copy an existing profile highlight the profile from...

Page 17: ...e default user login is Guest to set the default login as the last active user go to the Basic tab of the Settings dialog and uncheck the Default to Guest on startup option Note For multiple users we...

Page 18: ...iles list selected items appear blue Note If a USB keyboard is installed the virtual keyboard does not appear Pressing the Enter key on a physical keyboard is equivalent to clicking the Save button in...

Page 19: ...the parent folder and then click the New Folder button This activates the virtual keyboard if it is needed so you can enter a folder name and date Note Click the Date button anywhere within a field to...

Page 20: ...t describes the capture session The blue Next file name reflects the information entered 3 Click in the Count field to enter the starting number if you do not want to start at 1 The blue Next file nam...

Page 21: ...tomatically insert the current date MM DD YYYY wherever the cursor is in that field 7 Click OK to accept QuickSave settings 8 In the right control bar select the QuickSave option 9 After acquiring an...

Page 22: ...own 4 Click the Duration field and enter a value Choose a unit of measure for the duration of the capture session from the Duration Unit dropdown Note The Images field shows the total number of images...

Page 23: ...ng the current session Click Play to view all images in sequence Pause and restart a time lapse session 1 In the Time Lapse Progress dialog click Pause to suspend the time lapse session The progress d...

Page 24: ...bort a time lapse session 1 In the time lapseprogress dialog click Abort A new dialog opens and gives you the option to delete or keep the files already saved 2 To resume the session clickCancel to re...

Page 25: ...s desired the EVOS XL Imaging System will tag for the selected label 6 To move a tag select and drag it Left click anywhere else onscreen to deselect 7 To deletea tag right clickthetag Alternatively c...

Page 26: ...d and open the desired directory 4 Use the scroll bar as needed to search the preview list for the desired image file Click the image to select it The selected file name appears blue 5 Click View File...

Page 27: ...in to 2 To zoom in to an image at higher levels you must first capture it 1 Double click the area of interest in the image onscreen EVOS imaging system displays a view zoomed in at 2 centered on the...

Page 28: ...n The screen displays the zoomed in at 2 and centered on the clicked point Captured image at 40 magnification with scale bar Captured 40 image zoomed 2 2 In the enlarged image double click again on an...

Page 29: ...ssible to zoom in to the pixel level of the digital image Captured 40 image zoomed 8x 4 Right click to restore the view to unzoomed magnification Note Capturing and saving a zoomed image results in a...

Page 30: ...into the correct jack and connected via the adapter to the USB port 2 Be sure you are logged in under your own EVOS user ID The current user ID is displayed above the Invitrogen logo in the bottom lef...

Page 31: ...ed file appears on the file tree without a triangle icon and you are not able to expand or open it your permission to access that item is restricted 5 Enter your network domain user name and password...

Page 32: ...or more information on EVOS user IDs Your EVOS user ID remembers your network login it is encrypted in the instrument s non volatile memory All your connections and login information are hidden from o...

Page 33: ...ot open or rename the file on your computer EVOS XL Imaging System will verify and install it during the update procedure 2 Download the current user guide for the EVOS XL Imaging System from our webs...

Page 34: ...any devices during the update Set date and time To ensure accurate date and time information on saved image files verify your date time settings on the EVOS XL Imaging System before your capture sessi...

Page 35: ...vice the instrument yourself Unauthorized repairs may damage the instrument or alter its functionality which may void your warranty Contact your local EVOS distributor to arrange for service Objective...

Page 36: ...n peroxide H2O2 IMPORTANT Do not allow decontamination solution to get into lubricated areas such as the stage roller bearings or any points of rotation such as axles for the stage knobs condenser whe...

Page 37: ...stage be sure the sample s thickness is even Difficulty focusing on coverslipped sample on standard slide Place the slide so the coverslip is facing up long working distance objectives require a thic...

Page 38: ...power on the instrument Verify that the external display accepts DVI D input i e digital input A monitor with analog input only DVI A will not display the EVOS digital output Unable to connect to net...

Page 39: ...3 pos with brightfield and phasecontrast annuli Condenser working distance 60 mm Mechanical glide stage X Y axis fine positioning controls 28 3 mm 1 11 in per rotation 110 mm 110 mm 4 3 in 4 3 in ran...

Page 40: ...Left control bar The following functions are available in the left control bar bottom left LIGHT ON button Illumination slider Image capture button Color Adjustment button Adjust color dialog The Col...

Page 41: ...p the interval duration and the file name destination for time lapsesessions See Record time lapse images page 20 Count tool The Count tool allows you to tag cells and other features using up to 6 lab...

Page 42: ...me LCD Backlight controls the LCD lighting File Save section allows you to specify 8 bit as the default format for TIFF files Settings QuickSave tab Allows you to set up a file name count file type an...

Page 43: ...o include the current date in the field Click the Accept button when you have finished entering the text Note You can connect a USB keyboard to the EVOS XL Imaging System for text input When a keyboar...

Page 44: ...nstrument on or O to turn it off USB and DVI ports Plug the mouse and flash drive into the USB ports Use the DVI port for digital output to a projector or other display Coarse stage positioning knobs...

Page 45: ...Set the phase annuli selector to the position that corresponds with your selected objective for transmitted light observations The selector clicks into place for each of the following positions BF use...

Page 46: ...fety conventions used in this document Symbols on instruments Safety labels on instruments General instrument safety Chemical safety Chemical waste safety Electrical safety Physical hazard safety Biol...

Page 47: ...zardous situation that if not avoided may result in minor or moderate injury It may also be used to alert against unsafe practices WARNING Indicates a potentially hazardous situation that if not avoid...

Page 48: ...the Standby condition Hazardous voltage may be present if this switch is on standby Indicates the On Off position of a push push main power switch Indicates a terminal that may be connected to the si...

Page 49: ...e caution Indicates the presence of a laser inside the instrument and to proceed with appropriate caution Indicates the presence of moving parts and to proceed with appropriate caution Indicates the p...

Page 50: ...nation des d chets DANGER High voltage DANGER Haute tension WARNING To reduce the chance of electrical shock do not remove covers that require tool access No user serviceable parts are inside Refer se...

Page 51: ...t of the computer and or the monitor moving them may require two or more people Things to consider before lifting the computer and or the monitor Make sure that you have a secure comfortable grip on t...

Page 52: ...handles locked in the upright position Wear appropriate eyewear clothing and gloves when handlingreagent and waste bottles General safety guidelines To minimize the hazards of chemicals Read and under...

Page 53: ...othing For additional safety guidelines consult the SDS Minimize the inhalation of chemicals Do not leave chemical containers open Use only with adequate ventilation for example fume hood For addition...

Page 54: ...ace fuses only with fuses of the type and rating specified for the instrument Power DANGER ELECTRICAL HAZARD Grounding circuit continuity is vital for the safe operation of equipment Never operate equ...

Page 55: ...e U S U S Department of Health and Human Services guidelines published in Biosafety in Microbiological and Biomedical Laboratories stock no 017 040 00547 4 www cdc gov OD ohs biosfty bmbl4 bmbl4toc ht...

Page 56: ...A CSA America ANSI ASME ASSE ASTM NSF and UL European Safety and EMC Standards The CE Mark symbolizes that the product conforms to all applicable European Community provisions for which this marking i...

Page 57: ...information about customer training Download software updates and patches Safety Data Sheets SDS Safety Data Sheets SDSs are available at thermofisher com sds IMPORTANT For the SDSs of chemicals not d...

Page 58: ...thermofisher com support thermofisher com askaquestion thermofisher com 09 October 2017...

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