ibidi Universal Fit 10918 Instruction Manual Download Page 5

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ibidi Heating System, Universal Fit

Operation

5.1

Installation and Connection of the Parts

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5.2

Start the ibidi Heating System, Universal Fit

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5.3

Setting Parameters in the Front Display

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5.3.1

Run/Stop Mode

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5.3.2

Incubation Parameters

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5.3.3

Setup Menu

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5.4

Temperature Adjustment in the Sample

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5.5

Sample Preparation

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IncuControl Software

Maintenance

7.1

Disinfection and Cleaning

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7.2

Influence of Ambient Temperature and Ventilation

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Troubleshooting

8.1

Focus not Stable

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8.2

Evaporation Is too High

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8.3

Condensation Inside the Stage Top Incubator

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Instruction Manual

Version 2.2 (May 23, 2019)

Summary of Contents for Universal Fit 10918

Page 1: ...10918 ibidi Heating System Universal Fit for 1 Chamber Version 2 2 Instruction Manual ibidi Heating System Universal Fit Lid I 42 1 S 42 0 Run Plate Glass 37 0 37 0 N C 37 0 Setup N C ...

Page 2: ...ibidi Heating System Universal Fit Version 2 2 May 23 2019 2 Instruction Manual ...

Page 3: ...ystem Universal Fit Contact ibidi GmbH Lochhamer Schlag 11 82166 Gra felfing Germany Phone 49 89 520 46 17 0 Fax 49 89 520 46 17 59 E mail info ibidi de Internet ibidi com Instruction Manual 3 Version 2 2 May 23 2019 ...

Page 4: ... Heating System Universal Fit 13 1 11 Waste Disposal WEEE RoHS Compliance Statement 13 1 11 1 EU Directive WEEE 13 1 11 2 EU Directive RoHS 13 2 Intended Use 14 3 Principle 14 4 Equipment 15 4 1 Components of the ibidi Heating System Universal Fit 15 4 2 Combination Options 16 4 3 Temperature Controller 17 4 4 Heated Lid 18 4 5 Heated Plate 19 4 6 Heating Inserts 20 4 6 1 Heating Insert µ Slide 20...

Page 5: ... 3 2 Incubation Parameters 24 5 3 3 Setup Menu 24 5 4 Temperature Adjustment in the Sample 26 5 5 Sample Preparation 27 6 IncuControl Software 28 7 Maintenance 29 7 1 Disinfection and Cleaning 29 7 2 Influence of Ambient Temperature and Ventilation 29 8 Troubleshooting 30 8 1 Focus not Stable 30 8 2 Evaporation Is too High 30 8 3 Condensation Inside the Stage Top Incubator 30 Instruction Manual 5 ...

Page 6: ...N and NOTE have specific meanings in this man ual Do not proceed beyond a signal word until you have performed the indicated actions WARNING A potentially hazardous situation which if not avoided could result in se rious injury or even death Warning messages in the text are displayed in a gray shaded box CAUTION A potentially hazardous situation which if not avoided could result in minor or modera...

Page 7: ... to channel 1 Heated Lid Universal Fit DC 10 V max 5 A Output voltage to channel 2 Heated Plate Universal Fit for 1 Chamber DC 12 V max 5 A Operating and Storage Conditions Operating site Indoor use only Operating temperature 18 30 C 64 86 F min 5 C 9 F less than set temperature Operating humidity max 80 relative humidity RH Operating altitude max 2000 m atmospheric pressure 800 1060 hPa 11 6 15 4...

Page 8: ...g 0 11 lbs Weight blank holder 47 g 0 11 lbs Heating Insert Slides Height without blank holder 8 mm Height with blank holder 13 15 mm Observation area 49 mm 22 mm Weight insert 42 5 g 0 94 lbs Weight blank holder 38 5 g 0 85 lbs USB cable Length 1 8 m Power supply cable Length 2 0 m power supply to wall Length 1 2 m power supply to device Temperature Control Range Heated Lid Ambient temperature mi...

Page 9: ...der for standard multi well plates Optical Properties Glass Lid Glass Selected HQ Floatglass Thickness of the glass plate 1 1 mm Refractive index glass nD 1 520 588 nm ITO coating Thickness 100 nm Passivation layer Thickness 20 25 nm Refractive index ITO with passiva tion layer 1 95 Instruction Manual 9 Version 2 2 May 23 2019 ...

Page 10: ...able power cables by power cables with inadequate specifications By violating these instructions you risk electric shock and fire Only use extension cables that have a protective ground wire Do not operate the ibidi Heating System Universal Fit under conditions that pose a risk of explosion implosion or the release of gases Only operate the ibidi Heating System Universal Fit with aqueous solutions...

Page 11: ...nables easy access for mainte nance Do not place heavy objects on the instrument The heated glass plates of the incubation chamber can break on mechanical impact If so the glass shards can lead to injuries if handled Be aware that when switched on a 10 V DC voltage is applied to the underside of the glass on the Heated Lid Do not touch the underside or put it in contact with anything conductive Th...

Page 12: ...urrounding gases 7 Accidents beyond ibidi s control including natural disasters This warranty does not cover consumables such as cell culture chambers and dishes tubes fluidic connectors reagents etc The warranty for all parts supplied and repairs provided under this warranty expires on the warranty expiration date of the original product 1 9 Transporting the ibidi Heating System Universal Fit The...

Page 13: ...bidi Heating System Universal Fit must be disposed of in compliance with the WEEE Directive 2012 19 EC This symbol on the product is in accordance with the European Union s Waste Electrical and Elec tronic Equipment WEEE Directive The symbol indicates that this product must be recycled dis posed of separately from other household waste It is the end user s responsibility to dispose of this product...

Page 14: ...of a variety of microscopy labware rang ing from 35 mm dishes to regular slides 3 Principle Physiological Conditions in Live Cell Imaging The Heated Lid and Heated Plate are designed to keep cells on stage at 37 C in microscopy slides and dishes Figure 1 To achieve this the glass top of the Heated Lid and the Heated Plate are actively heated by the Temperature Controller The Heated Lid prevents co...

Page 15: ...em Universal Fit The components of the ibidi Heating System Universal Fit are listed below Description Drawing Temperature Controller Heated Lid with electrical cable and D sub connector to connect to the Temperature Controller Heated Plate with electrical cable and D sub connector to connect to the Temperature Controller Heating Insert µ Dish 35mm high Heating Insert µ Dish 35mm low Instruction M...

Page 16: ... in Figure 3 The Heated Lid and Heated Plate fit on an inverted microscope stage equipped with a universal mounting frame for multiwell plates They are connected to the Temperature Controller The ibidi Heating System Universal Fit can be combined with the Gas Mixer unit that provides CO2 and O2 optional control and a defined humidified atmosphere Detailed information on the Gas Mixer unit is given...

Page 17: ...rature Controller is designed to control the different heated components four channels available The front display shows the set values S and the current values I The settings can be adjusted via the control buttons Section 5 3 If you wish to control the Temperature Controller via PC use the IncuControl software Section 6 Instruction Manual 17 Version 2 2 May 23 2019 ...

Page 18: ... switch Temp Sensor Power Ch 1 Ch 2 Ch 3 Ch 4 24 V max 6 7 A USB ibidi Temperature Controller Type Serial number Production date ibidi Temperature Controller ibiTC2 001 11 2018 ibidi GmbH Am Klopferspitz 19 D 82152 Martinsried Germany 49 0 89520 46 17 0 www ibidi com Heated Lid Heated Plate Heated Glass Bottom Heated Plate 4 Slides 4 control channels Figure 5 Rear view of the Temperature Controlle...

Page 19: ...nd the humidity sensor Detailed information on the Gas Incubation System is given in its instruction manual Figure 6 Heated Lid 4 5 Heated Plate The Heated Plate provides the base for the ibidi Heating System Universal Fit The lower part of the Heated Plate fits into any microscope stage with a universal mounting frame for multiwell plates The electrical cable 1 5 m is connected to the Temperature...

Page 20: ...is to create a tight contact between the Slides or Dishes and the metallic insert to maximize the heat transfer and ensure stable heating of the sample The Heated Plate passively heats the inserts WARNING Heated Plate Heated Lid and Heating Inserts all contain strong neodymium magnets Please contact us for a non magnetic system if permanent magnetic fields are detrimental to your ex periment 4 6 1...

Page 21: ...e in order to snap the dishes into a fixed position This will leave an inclined gap between the holder and the insert as shown in Figure 10 but this is intentional and helps to keep the dishes in place Figure 10 Schematic Drawing of µ Dish in the Heating Insert To ensure a tight fit the lid of the Insert is tilted when closed Instruction Manual 21 Version 2 2 May 23 2019 ...

Page 22: ...Follow the instructions in Section 5 4 5 1 Installation and Connection of the Parts The components of the ibidi Heating System Universal Fit are connected as shown in Figure 11 Figure 11 Installation of the components of the ibidi Heating System Universal Fit NOTE Before shipment all controllers are run through an in house calibration with the correspond ing Heated Lid and Heated Plate Only use th...

Page 23: ...ee Section 4 3 The system immediately starts heating up the heated components WARNING Be aware that when the system is switched on 10V DC voltage is applied to the underside of the glass plate Do not touch the underside or contact it with anything conductive This could cause a short circuit that may destroy the controller and or the lid If you are not sure which temperature is set e g when operati...

Page 24: ... Universal Fit for 1 Chamber 10918 or Heated Plate Multi Well Plates K Frame 10929 Channel 3 Glass Heated Glass Bottom Multi Well Plates K Frame 10929 Channel 4 Ch4 Heated Plate Universal Fit for 4 µ Slides 10927 10928 5 3 1 Run Stop Mode Set the whole system to run or stop mode by manipulating the setting in the display s bottom line to Run system is running or Stop system is not running Figure 1...

Page 25: ...menu to the alarm menu by pressing the right button The maximum and minimum limits of the alarm can be set for each individual channel If the alarm is activated which happens when the current value goes under over the low high limits the current value I blinks showing alternately the value and low or high respectively To stop the display blinking navigate to the blinking channel and press the roun...

Page 26: ...low this procedure to adjust the sample temperature 1 Set all your peripheral experimental parameters such as room temperature air conditioning airflow illumination microscope settings etc 2 Connect the temperature sensor thermocouple type K to the plug on the Temperature Con troller s back 3 Fill the provided µ Dish or µ Slide depending on what you want to use for your experiments with water Volu...

Page 27: ...ubator Use of an Objective Heater 5 5 Sample Preparation Check that the cell cultureware you intend to use fits into the Insert of the ibidi Heating System Universal Fit Prepare the cells according to your protocol and place the culture vessel in the Heating Insert The Heating Insert can be removed from the ibidi Heating System Universal Fit even with the holder in place Then put the whole assembl...

Page 28: ...be downloaded from the ibidi website For more details please refer to the IncuControl instructions Figure 18 Temperature control window in IncuControl Prohibitions on the use of ibidi software Copying software for other than backup purposes Transfering or licensing of the right to use software to a third party Disclosure of confidential information regarding software Modification of software Versi...

Page 29: ...may remove the instrument s paint To clean the heated components and or the Temperature Controller switch off the Temperature Con troller and disconnect all electrical cables Leave the instrument to cool down for approx 5 minutes WARNING Hot surface max 55 C Do not touch Heated Lid Heated Plate and Heated Glass Bottom when hot 7 2 Influence of Ambient Temperature and Ventilation The ambient temper...

Page 30: ...t using silicone oil e g Anti Evaporation Oil ibidi 50051 to decrease evaporation Covering the medium with sterile silicone oil prevents all evaporation effects and is compatible with cell culture Please do not use mineral oil as this can be harmful to your cultureware Equilibrate oil and medium inside the incubator overnight This step helps to avoid the formation of air bubbles and pre warms the ...

Page 31: ...ibidi Heating System Universal Fit Instruction Manual 31 Version 2 2 May 23 2019 ...

Page 32: ...chhamer Schlag 11 82166 Gräfelfing Germany Toll free within Germany Phone 0800 00 11 11 28 Fax 0800 00 11 11 29 International calls Phone 49 89 520 46 17 0 Fax 49 89 520 46 17 59 E Mail info ibidi com www ibidi com ...

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