ibidi Heating System, Universal Fit
5.4
Temperature Adjustment in the Sample
The ibidi Heating System, Universal Fit controls the temperature of the actively heated components.
The temperature in the sample results from the interaction of all heated parts. Therefore, the temper-
ature at the position of the cells must be measured and adjustments should be made to the heated
components to achieve the desired temperature. An adjustment is recommended before starting the
first experiment. The temperature adjustment should be done for each chamber type, in order to
control temperatures with an absolute accuracy of less than 1°C.
When starting the temperature adjustment in the sample in your specific setup, use the recommended
temperature settings (for samples that should be 37°C):
Table 5 – Recommended temperature settings before optimization
Device
Temperature
Heated Lid (channel 1)
42°C
Heated Plate (channel 2)
38°C
To measure and calibrate the sample temperature, use the provided Temperature Adjustment Set.
The temperature of the heated components has to be adjusted in small steps. Follow this procedure
to adjust the sample temperature:
1. Set all your peripheral experimental parameters, such as room temperature, air conditioning,
airflow, illumination, microscope settings, etc.
2. Connect the temperature sensor (thermocouple type K) to the plug on the Temperature Con-
troller’s back.
3. Fill the provided µ–Dish or µ–Slide (depending on what you want to use for your experiments)
with water. Volumes should resemble the amount you need for your experiments.
4. Put the loose end of the thermocouple through the hole in the lid of the µ–Dish/µ–Slide and
make sure it dips into the water.
5. Place the µ–Dish/µ–Slide in the Insert and put the holder on.
6. Place the Insert in the ibidi Heating System, Universal Fit and close the Heated Lid.
7. After 30 minutes, check the temperature of the external sensor on the display or in the Incu-
Control software. If the sample temperature is still too low, raise the set values for the Heated
Plate and Heated Lid for 0.5-1°C and wait until the sample temperature is stable again (at least
10-15 min).
8. When the sample temperature has reached the desired value, write down the set values for
the Heated Plate and Heated Lid and use those settings for all upcoming experiments with the
same probe holder.
Version 2.2 (May 23, 2019)
26
Instruction Manual
