ibidi Heating System 4 Slides – Silver Line
ibidi
Gas Mixer
Gas Mixer
Humidification
Stage Top Incubator
Humidity
sensor
Valve
regulation
Gas mixture
(humidified)
Dry gas
Gas mixture
(dry)
Super-humidified gas
“100%+” RH
Feedback control
Air
CO
2
N
2
Dry gas mix
Humidified gas mix
Humidified and warm
gas mixture
ibidi
Temperature
Controller
Figure 2: Schematic view of the ibidi Stage Top Incubation System, combining the Heating System
with the Gas Incubation System. RH: relative humidity.
In combination with the Heating System, it supplies complete incubator conditions for your live cell
imaging directly on the microscope stage.
Humidity in ambient air can lead to condensation on all surfaces, especially on the lids of cell culture
vessels. If these surfaces are within the optical pathway, small water droplets will cause light scat-
tering. This diminishes the optical quality of transmitted light microscopy (i.e., phase contrast). To
ensure the highest image quality, condensation on any surface should be prevented during live cell
imaging experiments.
The independently controlled Heated Lid of the ibidi Heating System prevents condensation during
live cell imaging. By heating the lid to a temperature higher than the plate, a vertical temperature
gradient is created. This gradient and active humidity control prevent the formation of condensation
on the lid of the cell culture vessel.
34 °C
Unheated Lid
Heated Plate
37
°C
ibidi solution:
Heated Lid
40 °C
Heated Plate
37
°C
Figure 3: The Heating System prevents condensation on the cell culture vessel lid, as well as on the
Heated Lid itself.
Instruction Manual
15
Version 1.0 (August 1, 2022)
