ibidi 12130 Instruction Manual Download Page 3

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ibidi Heating System 4 Slides – Silver Line

Contact

ibidi GmbH

Lochhamer Schlag 11

82166 Gr¨afelfing

Germany

Phone: +49 89 / 520 46 17 - 0

Fax: +49 89 / 520 46 17 - 59

E-mail:

[email protected]

Internet:

ibidi.com

Instruction Manual

Version 1.0 (August 1, 2022)

Summary of Contents for 12130

Page 1: ...ibidi Heating System 4 Slides Silver Line Instruction Manual ibidi Heating System 4 Slides Silver Line Version 1 0 1 3 4 6 2 5 ibidiTemperature Controller 1 Plate I 38 0 S 38 0 Run Lid 42 1 42 0 Setup N C com ...

Page 2: ...ibidi Heating System 4 Slides Silver Line Version 1 0 August 1 2022 2 Instruction Manual ...

Page 3: ...m 4 Slides Silver Line Contact ibidi GmbH Lochhamer Schlag 11 82166 Gra felfing Germany Phone 49 89 520 46 17 0 Fax 49 89 520 46 17 59 E mail info ibidi de Internet ibidi com Instruction Manual 3 Version 1 0 August 1 2022 ...

Page 4: ...Heating System 4 Slides 12 1 10 Waste Disposal WEEE RoHS Compliance Statement 12 1 10 1 EU Directive WEEE 12 1 10 2 EU Directive RoHS 12 1 11 Regulatory Statement 13 2 Intended Use 14 3 Principle 14 4 Equipment 16 4 1 Components of the ibidi Heating System 4 Slides 16 4 2 Stage Top Incubator Overview 17 4 3 ibidi Temperature Controller Silver Line 18 4 4 Heated Plate and Magnetic Holder 19 4 5 Hea...

Page 5: ...cubation Parameters 24 5 3 3 Select Temperature Sensor 25 5 3 4 Setup Menu 25 5 4 Temperature Adjustment in the Sample 26 5 5 Sample Preparation 28 6 IncuControl Software 29 7 Maintenance 30 7 1 Disinfection and Cleaning 30 7 2 Influence of Ambient Temperature and Ventilation 30 8 Troubleshooting 31 8 1 Focus not Stable 31 8 2 Evaporation too High 31 8 3 Condensation Inside the Stage Top Incubator...

Page 6: ...Note that the signal words WARNING CAUTION and NOTE have specific meanings in this man ual Do not proceed beyond a signal word until you have performed the indicated actions Warning messages in the text are displayed in a gray shaded box Please see section 1 6 for general safety considerations WARNING A potentially hazardous situation which if not avoided could result in serious injury or even dea...

Page 7: ...ble 1 Specifications of the ibidi Heating System 4 Slides Electrical Specifications Power Supply Protection class I International protection marking IEC 60529 IP 20 Overvoltage category II Pollution degree 2 Input voltage and current AC 100 240 V 50 60 Hz 2 A 36 40 W Output voltage and current DC 24 V 6 67 A 160 W Electrical Specifications ibidi Temperature Controller Silver Line Protection class ...

Page 8: ...th Heated Lid Height 25 5 mm Weight 330 g 0 46 lbs USB cable Length 1 8 m Power supply cable Length 2 0 m power supply to wall Length 1 2 m power supply to device Temperature Control Range Heated Lid 5 degree above ambient temperature min 18 C to 45 C Accuracy 0 2 C at sensor location 5 C entire heated glass Heated Plate for 4 µ Slides 5 degree above ambient temperature min 18 C to 45 C Accuracy 0...

Page 9: ...e action by uninstalling the conflicting product s ibidi is a registered trademark of ibidi GmbH in Germany and other countries 1 6 Safety Considerations WARNING Only operate the ibidi Heating System 4 Slides with the supplied components Only use the cables and plugs delivered with the system The power plug of the control unit must be inserted in an outlet with a ground earth contact Do not replac...

Page 10: ...ed the external power supply should not be used Avoid strong magnetic fields and sources of high frequency The ibidi Heating System 4 Slides might not function properly when located near a strong magnetic field or high frequency source Avoid vibrations from vacuum pumps centrifuges electric motors processing equipment and machine tools Avoid dust and corrosive gas Do not install the ibidi Heating ...

Page 11: ...idents beyond ibidi s control including natural disasters This warranty does not cover consumables such as cell culture chambers and dishes tubes fluidic connectors reagents etc The warranty for all parts supplied and repairs provided under this warranty expires on the warranty expiration date of the original product 1 8 Transporting the ibidi Heating System 4 Slides The weight of the ibidi Temper...

Page 12: ...nces RoHS 1 10 1 EU Directive WEEE The ibidi Heating System 4 Slides must be disposed of in compliance with the WEEE Directive 2012 19 EC This symbol on the product is in accordance with the European Union s Waste Electrical and Elec tronic Equipment WEEE Directive The symbol indicates that this product must be recycled dis posed of separately from other household waste It is the end user s respon...

Page 13: ...ation Laborgerät laboratory equipment ibidi Temperature Controller ibiTC3 XXX with ibidi accessories Der oben beschriebene Gegenstand erfüllt die Vorschriften der Richtlinie 2011 65 EU vom 08 Juni 2011 zur Beschränkung der Verwendung bestimmter gefährlicher Stoffe in Elektro und Elektronikgeräten The object of the declaration described above is in conformity with Directive 2011 65 EU of 8 June 201...

Page 14: ...l Imaging Cells react sensitively to changes in their environment Factors such as temperature humidity and CO2 O2 levels significantly influence the outcome of cell culture assays In order to achieve biologi cally relevant and reproducible results it is crucial to maintain optimal conditions on the microscope stage during live cell imaging experiments To enable high quality live cell imaging under...

Page 15: ...cell culture vessels If these surfaces are within the optical pathway small water droplets will cause light scat tering This diminishes the optical quality of transmitted light microscopy i e phase contrast To ensure the highest image quality condensation on any surface should be prevented during live cell imaging experiments The independently controlled Heated Lid of the ibidi Heating System prev...

Page 16: ... 1 42 0 Setup E N C Heated Lid with electrical cable to the converter box Heated Plate for 4 µ Slides with electrical cable to the converter box Converter box connected to the cables of the Heated Plate and Heated Lid with multipole connector to the ibidi Tem perature Controller Silver Line Magnetic Holder to fix the slides on the Heated Plate Temperature Adjustment Set 1 temperature sensor 1 perf...

Page 17: ...ormation on the ibidi Gas Incubation System Silver Line is given in the Gas Incubation System instructions Heated Plate Magnetic Holder Your inverted microscope Existing stage or frame for multiwell plates on your microscope Heated Lid ibidi µ Slide ibidi Gas Mixer Flow I 9 9 S 10 0 Run CO2 Hum 5 1 5 0 75 2 80 0 Setup 1 3 4 6 2 5 ibidi Temperature Controller 1 Plate I 38 0 S 38 0 Run Lid 42 1 42 0...

Page 18: ...ver Line In total 6 channels are available each channel accommodating one heated component e g the Heated Lid and Heated Plate of the Incubation Chamber 4 Slides In addition an external Temperature Sensor can be connected The front panel enables access to the Temperature Controller s settings The front panel display shows the values of set S set and actually measured I is temperatures The temperat...

Page 19: ...1 2021 ibidi GmbH Am Klopferspitz 19 D 82152 Martinsried Germany 49 0 89520 46 17 0 www ibidi com Temp Sensor Power Signal Out 1 2 3 6 4 5 Output Channels 24 V max 6 7 A USB Electrical cable to Gas Mixer Ports to heating devices On Off Figure 6 Rear view of the ibidi Temperature Controller Silver Line 4 4 Heated Plate and Magnetic Holder The Heated Plate provides the base for the ibidi Heating Sys...

Page 20: ...ers with a working distance of 26 mm The upper glass part of the Heated Lid is heated The electrical cable is connected to the ibidi Tem perature Controller Silver Line The Heated Lid fits exactly and securely onto the Heated Plate For gas incubation the Heated Lid is equipped with inlets for the gas flow and the humidity sensor Detailed information on the Gas Incubation System is given in its ins...

Page 21: ... the clip define the optimal position of the clip on your stage before removing the protective foil from the self adhesive tape and mounting the clip to the stage by slight pressure It takes about one hour until full adhesion strength of the tape has been reached Open the clamp and introduce all cables and tubing leading from the Incubation Chamber to the controller s If there is no more need for ...

Page 22: ...idi Temp Controller Heated Tubing Package Electrical cable Gas tubing Computer optional USB cable Temperature Controller cable Humidity sensor cable Incubation Chamber Figure 11 Installation of the components of the ibidi Heating System 4 Slides 1 Place the ibidi Temperature Controller Silver Line next to the microscope and connect the power supply 2 Confirm that the power switch of the Temperatur...

Page 23: ...side of the glass plate Do not touch the underside or contact it with anything conductive This could cause a short circuit that may destroy the controller and or the lid If you are not sure which temperature is set e g when operating the system the first time it is recommended to check the settings without your sample 5 3 Setting Parameters in the Front Display All control parameters can be manual...

Page 24: ... tion Chamber Dish Slide Channel 4 5 and 6 Plate Lid Glass or Incubation Chamber Multiwell Plate 4 Channel 6 Obj Objective Heater Universal The first three lines of the display show the respective set S set and actual I is temper ature values of the corresponding components Figure 14 If a component of a heating unit is not connected properly N C will be shown in the I value line The bottom line sh...

Page 25: ...t temperature sensor A No temperature sensor is selected B Select the thermo couple type K TC K as the temperature sensor by using the set buttons 5 3 4 Setup Menu The Setup menu offers the possibility to change the settings of the individual channels and the dis play For standard operation no changes need to be made Enter the setup menu by navigating to the Setup entry and pressing the round butt...

Page 26: ...the preferences menu Preferences Setup Backlight 65 Contrast 15 Return Figure 18 Preferences dialog Info Information about the serial number and firmware version is shown in the info dialog Info Setup 2 SN ibiTC30018 FW 3 1 1 TC Figure 19 Info display 5 4 Temperature Adjustment in the Sample The ibidi Temperature Controller Silver Line controls the temperature of the actively heated com ponents He...

Page 27: ...he lid of the culture vessel and make sure it dips into the liquid 5 Place the µ Slide with the temperature sensor and three further slides into the Heated Plate 6 Close the Incubation Chamber with the Heated Lid 7 After 30 minutes check the temperature of the external sensor on the display or in the Incu Control software If the sample temperature is still too low raise the set values for the Heat...

Page 28: ... Heated Plate mounted on the microscope stage Close the Heated Lid NOTE Let the temperature of the system equilibrate for a minimum of 30 min before you start your experiments NOTE Working with high aperture objectives might limit the observation area due to their large diameter In this case ibidi recommends leaving out the outermost rows of wells to avoid the risk of objective collision with the ...

Page 29: ...oaded from the ibidi website For more details please refer to the IncuControl instructions Figure 20 Temperature control window in IncuControl Prohibitions on the use of ibidi software The following actions are prohibited Copying software for other than backup purposes Transferring or licensing of the right to use software to a third party Disclosure of confidential information regarding software ...

Page 30: ...may remove the instrument s paint To clean the heated components and or the Temperature Controller switch off the ibidi Temperature Controller Silver Line and disconnect all electrical cables Leave the instrument to cool down for approx 5 minutes WARNING Hot surface max 55 C Do not touch Heated Lid Heated Plate and Heated Glass Bottom when hot 7 2 Influence of Ambient Temperature and Ventilation T...

Page 31: ...dditionally we suggest using silicone oil e g Anti Evaporation Oil ibidi 50051 to decrease evaporation Covering the medium with sterile silicone oil prevents all evaporation effects and is compatible with cell culture Please do not use mineral oil as this can be harmful to your cultureware Equilibrate oil and medium inside the incubator overnight This step helps to avoid the formation of air bubbl...

Page 32: ...chhamer Schlag 11 82166 Gräfelfing Germany Toll free within Germany Phone 0800 00 11 11 28 Fax 0800 00 11 11 29 International calls Phone 49 89 520 46 17 0 Fax 49 89 520 46 17 59 E Mail info ibidi com ibidi com com ...

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