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Hanna Instruments HI 5421, Instruction Manual

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All rights are reserved. Reproduction in whole or in part is prohibited without the written consent of the 

copyright owner.

Thank you for choosing a Hanna Instruments product.
Please read this instruction manual carefully before using this instrument. This 
manual will provide you with the necessary information for correct use of this 
instrument, as well as a precise idea of its versatility.
If you need additional technical information, do not hesitate to e-mail us at 

[email protected]

 or view our worldwide contact list for a Hanna Instruments  

representative near you at 

www.hannainst.com

.

Dear 
Customer

Summary of Contents for HI 5421

Page 1: ...INSTRUCTION MANUAL HI5421 DO BOD OUR SOUR Temperature Bench Meter...

Page 2: ...manual carefully before using this instrument This manual will provide you with the necessary information for correct use of this instrument as well as a precise idea of its versatility If you need a...

Page 3: ...TION SPECIFICATIONS OPERATIONAL GUIDE DISPLAYING MODES SYSTEM SETUP DO SETUP BOD SETUP OUR SETUP SOUR SETUP DO CALIBRATION PRESSURECALIBRATION DO MEASUREMENT BOD MEASUREMENT OUR MEASUREMENT SOUR MEASU...

Page 4: ...rature sensor HI7041S Electrolyte solution 30 mL Membrane caps 2 pcs HI76404W Electrode Holder 12 Vdc Power Adapter Electrode protective cap Instruction Manual and Quick Reference Guide Certificate HI...

Page 5: ...reeze the stable reading on the LCD DO only Two selectable alarm limits for DO BOD OUR SOUR Three selectable logging modes Automatic Manual or AutoHold DO only Up to 100 logging lots for automatic or...

Page 6: ...6 HI5421 DESCRIPTION FRONT PANEL REAR PANEL 1 Liquid Crystal Display LCD 2 Capacitive touch keypad 3 ON OFF button 4 Power adapter socket 5 DO probe input 6 USB connector FUNCTIONAL DESCRIPTION...

Page 7: ...yed function depending on the current menu e g and in Measure mode LCD GENERAL DESCRIPTION To enter exit calibration mode To select the desired measurement mode DO BOD OUR SOUR To enter Setup System S...

Page 8: ...L 0 0 to 42 0 psu BOD Biochemical Oxygen Demand Yes OUR Oxigen Uptake Rate Yes SOUR Specific Oxigen Uptake Rate Yes Keyboard 8 keys capacitive touch Probe Polarographic with temperature built in PC i...

Page 9: ...T START UP Please ensure that the capacitive keypad is not covered by hand or other objects at the meter power on Turn the instrument on from the power button located on the rear panel of the instrume...

Page 10: ...de The Choose Display Configuration message will be displayed in the Reminder messages area Press The instrument will display the basic information for the selected measurement mode GLP Accessing this...

Page 11: ...be displayed in the Reminder messages area Press Press to begin online graph To Zoom Graph Press then and will appear in virtual keys Use and to move along the X Time axis of the graph Press to acces...

Page 12: ...n Measure mode The Choose Display Configuration message will be displayed in the Reminder messages area Press The instrument will display the log history regarding the selected Measure mode Notes When...

Page 13: ...a System Setup option Use or to highlight the desired option Press to access the selected option The following is a detailed description of the System Setup option screen Beeper This option allows th...

Page 14: ...name location or number Company Name used to include the Company ID to the GLP data field Additional Info two data fields are available for general notes or notations To add the GLP Data Press while i...

Page 15: ...at from 7 available options DD MM YYYY MM DD YYYY YYYY MM DD YYYY MM DD Mon DD YYYY DD Mon YYYY or YYYY Mon DD To set the Date Time Press while in Measure mode Press Use or to select the Date Time opt...

Page 16: ...desired parameter Use or toadjustthecontrast backlight or to set the desired backlight saver time Press toconfirmthemodifiedoptionsandreturn to the System Setup menu Color Palette Thisoptionallowstheu...

Page 17: ...option allows the user to choose the desired language in which all informations will be displayed To select the Language Press while in Measure mode Press Use or to select the Language option Press an...

Page 18: ...eral information about the instrument serial number each instrument has an unique identification serial number the software version and the factory calibration date and time Note All instruments are f...

Page 19: ...ss toconfirmyourselection Apop upmenu will be displayed asking for confirmation Press toconfirmyourselectionandreturntothe System Setup or press to return to the System Setup menu without restoring de...

Page 20: ...d at a moment s notice A profile is a sensor setup complete with measurement units logging and display preferences calibration standards setup of the Display screen for measurement i e graphing GLP an...

Page 21: ...name by using and to highlight the desired character and then press to add it to the text bar It is also possible to delete the last character by positioning the cursor on the Backspace character and...

Page 22: ...d press Press to return to previous menu Reading Mode This option allows the user to select between Direct or Direct AutoHold DO reading modes To set the reading mode Press while in DO mode Press Use...

Page 23: ...sius Fahrenheit or Kelvin unit Press toconfirmyourselectionorpress to cancel operation Calibration This selection allows configuration of options pertaining to calibration Standard Recognition The use...

Page 24: ...operation Set Reminder Period Daily reminder the user can set the time from the day when the reminder is to appear Periodic reminder the user can set the time from the last calibration days hours and...

Page 25: ...ess toconfirmyourselectionorpress to cancel operation Clear Calibration Accessing this option the existent DO calibration can be cleared If the calibration is cleared a default meter calibration is us...

Page 26: ...e pressure source Select or using virtual key Press to return to previous menu Note IfManualpressuresourceischosen thepressure value used to compute is set manually If Automatic pressure source is cho...

Page 27: ...n of measurement unit and value Salinity Compensation is used when using DO con centration measurements mg L or ppm Oxygen solubility decreases when water contains salt Note Selectsalinityunitbeforese...

Page 28: ...to assign an identification number name to sample logs Two Sample ID parameters are available ID Increment mode and Edit Sample ID ID Increment Choose None to identify a sample with a text tag Choose...

Page 29: ...rent value or press to cancel operation Stability criteria This parameter permits the user to select a signal stability criteria for the measurement Stable tag appears on display Fast permits quicker...

Page 30: ...y at constant time intervals Manual a snapshot of the displayed measurement data is logged with time stamp when the user manually depresses Log Auto Hold this is configured along with the Direct AutoH...

Page 31: ...le Press to enable the parameter or to disable it Press to return to previous menu Sampling Period This option allows the user to select the desired sampling period for automatic logs To set the Sampl...

Page 32: ...press to escape without saving and return to the Log options Alarm This option allows the user to select the alarm settings Alarm State and Alarm Limits If the Alarm option is enabled a continuous do...

Page 33: ...or highlightthe Alarm Limits option Press andthenuse or tosetthe desired value then press to save the modified value or press to cancel operation Press return to the Alarm options Press and use or hig...

Page 34: ...option Use or to select the desired option Press to confirm your selection The following is a description of the BOD Setup option screens Profile see DO Setup section Temperature Unit see DO Setup se...

Page 35: ...ight the desired option Press to access the selected option The following is a description of the OUR Setup option screens Profile see DO Setup section Temperature Unit see DO Setup section Measuremen...

Page 36: ...Use or to highlight the desired option Press to access the selected option The following is a description of the SOUR Setup options Profile see DO Setup section Temperature see DO Setup section Measu...

Page 37: ...the current pressure temperature and salinity Initial preparation Make sure the probe is ready for measurements i e the membrane is filled with electrolyte and the probe is connected to the meter and...

Page 38: ...tip and allow measurement to stabilize on meter Procedure Suspend probe with membrane just over beaker of water for calibration in 100 Saturation Press and wait for Stable to appear on display Display...

Page 39: ...ibration Use or to modify the pressure value Press to finish the calibration or to exit calibration Note Use a reference pressure measurement if calibrating meter s sensor When the user standard is se...

Page 40: ...easurements a water movement of 0 3 m s is required This is to ensure that the oxygen depleted membrane surface is constantly replenished A moving stream will provide adequate circulation If the readi...

Page 41: ...44 46 4 10 11 28 10 58 9 93 9 32 9 03 50 0 12 10 77 10 11 9 50 8 92 8 65 53 6 14 10 29 9 68 9 10 8 55 8 30 57 2 16 9 86 9 28 8 73 8 21 7 97 60 8 18 9 45 8 90 8 39 7 90 7 66 64 4 20 9 08 8 56 8 07 7 60...

Page 42: ...8 6 5 6 3 6 0 5 8 5 7 64 4 20 9 1 8 8 8 4 8 1 7 8 7 5 7 3 7 0 6 7 6 5 6 2 6 0 5 8 5 6 5 5 68 0 22 8 7 8 4 8 1 7 8 7 5 7 2 7 0 6 7 6 5 6 2 6 0 5 8 5 6 5 4 5 3 71 6 24 8 4 8 1 7 8 7 5 7 2 7 0 6 7 6 5 6...

Page 43: ...reading is to be used then the manual pressure feature must be enabled See DO Setup The table below contains a conversion altitude m to pressure mmHg for the altitude values from the previous table D...

Page 44: ...ave been made for the degree of dilution and seed addition is called the BOD5 Before running a BOD measurement remember to set the BOD method configuration from the BOD setup menu and make sure the pr...

Page 45: ...ted following Operational Procedures DAY 5 Final DO Remove samples and Seed samples from the incubator for analysis Press then select Calibrate the DO probe Press then select Press Press toaccesstheli...

Page 46: ...required then press otherwise press Remove probe from sample and rinse with purified water Press to analyze another prepared sample or seed The list will reflect the 5 day analysis Note No Seed correc...

Page 47: ...h 5 day BOD determinations and 5 day BOD determina tions with seed corrections The dates reflect the last date the sample was evaluated Press then to select the sample and seed bottles If more than on...

Page 48: ...ults with SEED correction will be displayed Press to save and replace the previous sample report with this bottle ID or press to keep the previous sample BOD with no seed correction To print a copy of...

Page 49: ...total volume sample volume Dilution factor of sample Before starting an OUR measurement remember to set the OUR configuration from the OUR setup menu and make sure the instrument has been calibrated i...

Page 50: ...n The SOUR value is corrected to 20 C 68 F according to the Farrel and Bhide equation SOUR20 SOURT x 20 T Where T is the measured temperature in C and is a temperature dependent variable 1 05 for T ab...

Page 51: ...w Lot is selected under Log options Note When the is pressed the lot ID along with the current record number will appear for short time on the selected channel window on the top left corner e g L001_D...

Page 52: ...d with an H 5 Direct AutoHold Reading Mode and Auto Hold Log Press and then keys to initiate and automate the capture of stable data which is stored in the Recall Manual Log file During the process Au...

Page 53: ...ther with GLP information last calibra tion date and calibrated standards if a calibration has been performed on the selected mode and the logged values measured value temperature value temperature co...

Page 54: ...the selected lot or all lots The Please wait message will be displayed on the LCD until the selected lot or all lots are deleted Press and then press to exit deleting mode and return to Log Recall vi...

Page 55: ...tandard USB cable to connect your instrument to the PC Make sure that the instrument and the HI92000 software have the same baud rate and the ap propriate communication port The PC software may also b...

Page 56: ...prior to use Fillthecapwithcleanelectrolytesolution Gentlytapthesides of the membrane cap to ensure that no air bubbles remain trapped Do not tap the bottom directly as this will damage the membrane...

Page 57: ...brate the meter Check the sample is within the measurable range The meter fails to calibrate or gives faulty readings The probe is damaged Replace the probe The instrument doesn t measure the temperat...

Page 58: ...rm to the CE European Directives Disposal of Electrical Electronic Equipment The product should not be treated as household waste Instead hand it over to the appropriate collection point for the recyc...

Page 59: ...or replacement free of charge Damage due to accidents misuse tampering or lack of prescribed maintenance is not covered If service is required contact your local Hanna Instruments Office If under war...

Page 60: ...World Headquarters Hanna Instruments Inc Highland Industrial Park 584 Park East Drive Woonsocket RI 02895 USA www hannainst com MAN5421 Printed in ROMANIA...

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