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DO CALIBRATION

DO CALIBRATION

DO CALIBRATION

DO CALIBRATION

DO CALIBRATION

It is recommended to calibrate the instrument frequently, especially if high accuracy is required.
The DO range should be recalibrated:
• Whenever the DO probe is replaced.
• At least once a week.
• Before BOD, OUR, SOUR measurements.
• After testing aggressive chemicals.
• When calibration reminder is activated (“DO Cal Expired”).
• If the readings are far from the calibration point.

Note:

BOD, OUR and SOUR readings are automatically derived from the DO readings and no specific

calibration is needed.

The following options are available for the Dissolved Oxygen calibration:

• 

one point automatic zero calibration at 0% saturation or 0 mg/L

• 

one point automatic slope calibration at 100% saturation or 8.26 mg/L

• 

1 point manual calibration using a standard value set by the user in % saturation or mg/L

When automatic calibrations are performed it is assumed that the standard value is the saturated DO value at 25 °C,
0 g/L salinity and 760 mmHg.
When manual calibrations are performed it is assumed that the standard value is the DO value at the current
pressure, temperature and salinity.

Initial preparation

Make sure the probe is ready for measurements, i.e. the membrane is filled with electrolyte and the probe is
connected to the meter.
For an accurate calibration, it is recommended to wait for at least 15 minutes to ensure precise conditioning of
the probe.
Remove the protective cap from the DO probe.
Make sure the salinity value has been set to the salinity of the standard.

Probe conditioning

At

 startup, the probe is under polarization with a fixed voltage of approximately 800 mV for 1 minute.

Probe polarization is essential for stable measurements with the same recurring degree of accuracy.
With the probe properly polarized, oxygen is continually consumed when it passes through the sensitive
diaphragm and dissolves in the electrolyte solution contained in the probe.
Whenever measurements are taken with a non-polarized probe, the oxygen level revealed is both that of the
tested solution, as well as that present in the electrolyte solution. This measurement is 

incorrect

.

Summary of Contents for HI 4421

Page 1: ...1 www hannainst com HI 4421 DO BOD OUR SOUR Temperature Bench Meter Instruction Manual...

Page 2: ...Hanna Instruments first obtain a Returned Goods Authorization number from the Technical Service Department and then send it with shipping costs prepaid When shipping any instrument make sure it is pro...

Page 3: ...AL DESCRIPTION 5 SPECIFICATIONS 7 OPERATIONAL GUIDE 8 DISPLAYING MODES 9 SYSTEM SETUP 12 DO SETUP 18 BOD SETUP 29 OUR SETUP 31 SOUR SETUP 33 DO CALIBRATION 35 PRESSURE CALIBRATION 37 DO MEASUREMENT 38...

Page 4: ...modes are Basic information only GLP information Graph and Log History mode The main features of the instruments are One input channel Six measurement parameters DO BOD OUR SOUR pressure and temperat...

Page 5: ...tal Display LCD 2 Main Keyboard 3 USB connector 4 ON OFF switch 5 Power adapter socket 6 RS232 serial communication connector 7 DO probe input FUNCTIONAL DESCRIPTION FUNCTIONAL DESCRIPTION FUNCTIONAL...

Page 6: ...the displayed function depending on the current menu e g and in Measure mode LCD GENERAL DESCRIPTION To enter exit calibration mode To select the desired measurement mode DO BOD OUR SOUR To enter Set...

Page 7: ...U n e g i x O c i f i c e p S R U O S s e Y d r a o b y e K s y e k l a u t r i v 4 s y e k 8 e b o r P n i t l i u b e r u t a r e p m e t h t i w c i h p a r g o r a l o P e c a f r e t n i C P d e...

Page 8: ...of the instrument INSTRUMENT START UP Turn the instrument on from the power switch located on the rear panel of the instrument Please wait until the instrument finishes the initialization process Not...

Page 9: ...will be displayed in the Reminder messages area Press The instrument will display the basic informa tion for the selected measurement mode GLP Accessing this option a detailed GLP data will be display...

Page 10: ...y Configuration message will be displayed in the Reminder messages area Press When the online graph is displayed Use and to move the graph along the horizontal Time axis Press to access the zoom menu...

Page 11: ...e Dis play Configuration message will be displayed in the Reminder messages area Press The instrument will display the log history regarding the selected measure mode Notes When an alarm condition is...

Page 12: ...beeper is enabled a specific beep will be heard when the reading becomes stable when an alarm condition is reached when pressing a key or if a wrong key is pressed Stability Indicator When the reading...

Page 13: ...celingthesavingoperation and return to the editing mode by pressing If disabled the modified values will be saved automatically To enabled disabled the saving confirmation Use or toselecttheSavingConf...

Page 14: ...Date Time This option allows the user to set the current date time and the format in which they appear These parameters will be displayed on the Measure screens and also when storing measured data Set...

Page 15: ...notifying the user that a date time conflict has occured and some time dependent features could work improperly e g GLP Log LCD Setup This option allows the user to set the Contrast the Backlight of t...

Page 16: ...m Setup menu without changing Serial Communication This option allows the user to set the desired speed for the serial communication baud rate between the instrument and PC from 1200 2400 4800 or 9600...

Page 17: ...to inform the user that a new factory calibration is required To view the meter information Use or toselecttheMeterInformationoption Press to confirm and to view the meter information or press to ret...

Page 18: ...cess a DO setup options Use or to highlight the desired option Press to access the selected option or to exit setup The following is a detailed description of the DO Setup option screens Profile Choos...

Page 19: ...rent Profile To save the current profile Use or to select the Profile option Press and then select Save Current Profile option The text editor box will be displayed on the LCD Enterthedesiredprofilena...

Page 20: ...o highlight the desired option Press to confirm your selection or press to cancel operation Delete Profile To delete one of the existing profiles Use or to select the Profile option Press and use or t...

Page 21: ...option Use or to highlight the Barometer option from the DO Setup menu Press to access the Barometer option Pressure Source The user can choose between Manual and Automatic pressure source To set the...

Page 22: ...re units To set the pressure unit Use or to highlight the Pressure Units option Press and then use or to select between mmHg and mbar units Press to confirm your selection or press to cancel operation...

Page 23: ...or User Standard Use or to highlight the Standard Recognition option Use or to choose from Automatic or User Standard option Press to confirm your selection or press to cancel operation Calibration R...

Page 24: ...previous entry to be edited Press and use or to set the desired value then press to save the modified value Press to return to the previous menu Clear Calibration Accessing this option the existent D...

Page 25: ...mented at every new log lot notification To select the ID increment mode Use or to highlight the ID Increment option Press and then use or to highlight the desired option Press to confirm your selecti...

Page 26: ...e settings related to the logging feature as following Logging Type Three logging types are available Automatic Manual and AutoHold Automatic logging the readings are logged automatically at constant...

Page 27: ...ng Period This option allows the user to select the desired sampling period for automatic logging To set the sampling period Use or tohighlighttheSamplingPeriodoption Press and use or to select the de...

Page 28: ...t limits To set the alarm state Use or to highlight the Alarm State option Press and use or to highlight the desired option Press to confirm your selection or press to cancel operation Alarm Limits Th...

Page 29: ...The following is a description of the Resistivity Setup option screens Profile see DO Setup section Temperature Units see DO Setup section Measurement Unit see DO Setup section Method Configuration T...

Page 30: ...30 Barometer see DO Setup section Salinity see DO Setup section Sample ID see DO Setup section Log see DO Setup section Alarm see DO Setup section...

Page 31: ...Setup option Use or to highlight the desired option Press to access the selected option The following is a description of the OUR Setup option screens Profile see DO Setup section Temperature Units s...

Page 32: ...32 Barometer see DO Setup section Salinity see DO Setup section Sample ID see DO Setup section Log see DO Setup section Alarm see DO Setup section...

Page 33: ...o access the selected option The following is a description of the SOUR Setup options Profile see DO Setup section Temperature see DO Setup section Method Configuration This option allows the user to...

Page 34: ...34 Barometer see DO Setup section Salinity see DO Setup section Sample ID see DO Setup section Log see DO Setup section Alarm see DO Setup section...

Page 35: ...C 0 g L salinity and 760 mmHg When manual calibrations are performed it is assumed that the standard value is the DO value at the current pressure temperature and salinity Initial preparation Make sur...

Page 36: ...lize Youcandeleteapreviouslyperformedcalibrationbypressing When the automatic standard recognition is selected The calibration point will be automatically selected from the two standards available Pre...

Page 37: ...ATION If Automatic pressure source is chosen from the Pressure Source menu see DO Setup a pressure calibration in one point can be performed To perform pressure calibration Press to clear the current...

Page 38: ...curate DO measurements a water movement of 0 3 m s is required This is to ensure that the oxygen depleted membrane surface is constantly replenished A moving stream will provide adequate circulation I...

Page 39: ...1 7 7 0 1 1 1 0 1 0 5 9 2 9 8 5 6 8 6 3 5 4 1 9 2 0 1 8 6 9 0 1 9 5 5 8 0 3 8 2 7 5 6 1 6 8 9 8 2 9 3 7 8 1 2 8 7 9 7 8 0 6 8 1 5 4 9 0 9 8 9 3 8 0 9 7 6 6 7 4 4 6 0 2 8 0 9 6 5 8 7 0 8 0 6 7 8 3 7 0...

Page 40: ...8 0 6 8 1 5 9 1 9 8 8 5 8 1 8 8 7 6 7 3 7 0 7 8 6 5 6 3 6 0 6 8 5 7 5 4 4 6 0 2 1 9 8 8 4 8 1 8 8 7 5 7 3 7 0 7 7 6 5 6 2 6 0 6 8 5 6 5 5 5 0 8 6 2 2 7 8 4 8 1 8 8 7 5 7 2 7 0 7 7 6 5 6 2 6 0 6 8 5 6...

Page 41: ...al pressure feature must be enabled See DO Setup The table below contains a conversion altitude m to pressure mmHg for the altitude values from the previous table e d u t i t l A m 0 0 0 3 0 0 6 0 0 9...

Page 42: ...at 20 C in the dark and tested for dissolved oxygen DO after five days The loss of dissolved oxygen in the sample once correction have been made for the degree of dilution is called the BOD5 Before r...

Page 43: ...t screen If the time difference between the current reading and the selected reading is more than 1 day appears in stead and the BOD value can be calculated Press to display the BOD value If there are...

Page 44: ...lected Bottle ID list Press to delete a selected method from the Selected Bottle ID list Press to switch between the two lists When in Selected Bottle ID list press to access additionals options corre...

Page 45: ...sample volume Dilution factor of sample Before starting an OUR measurement remember to set the OUR configuration from the OUR setupmenu and make sure the instrument has been calibrated on DO To measur...

Page 46: ...he SOUR value is corrected to 20 C 68 F according to the Farrel and Bhide equation SOUR20 SOURT x 20 T Where T is the measured temperature in C and is a temperature dependent variable 1 05 for T above...

Page 47: ...be stored at the set sampling period Note While automatic logging is running the measured pa rameter setup is not available A warning message will be displayed if the setup is accessed If accessing G...

Page 48: ...2 DO only This logging mode can be used for multiple samples measurement By choosing this logging mode and will be available in Measure mode To log data using this mode Press while in Measure mode to...

Page 49: ...riterion is reached the logged value is the one that has been frozen on the LCD To store another frozen value press to return to normal logging mode and then again The records will be stored in one lo...

Page 50: ...he Choose Log Report Type message will be displayed in the Reminder messages area Press or to select the desired Log Report type All logged lots for the selected Log Report type will be displayed on t...

Page 51: ...d then press or to delete the selected lot or all lots The Please wait message will be displayed on the LCD until the selected lot or all lots are deleted Press and then press to exit deleting mode an...

Page 52: ...replace the membrane or refill with electrolyte proceed as follows Remove the protective cap by gently twisting and pulling and pulling it off the body of the probe see fig 1 Unscrew the membrane cap...

Page 53: ...o r p e h T s i r o s n e s e r u t a r e p m e t n e k o r b e b o r p e h t e c a l p e R e r a s g n i n r a w t i c i l p x E g n i r u d d e y a l p s i d n o i t a r b i l a c d e g a m a d y t...

Page 54: ...bottle HI 8061L General Cleaning Solution 500 mL FDA approved bottle OTHER ACCESSORIES OTHER ACCESSORIES OTHER ACCESSORIES OTHER ACCESSORIES OTHER ACCESSORIES HI 76408 DO probe for laboratory use wit...

Page 55: ...or to follow all necessary steps to correct interferences During operation ESD wrist straps should be worn to avoid possible damage to the probe by electrostatic discharges Any variation introduced by...

Page 56: ...Fax 210 884 0210 Indonesia Tel 21 4584 2941 Fax 21 4584 2942 Japan Tel 03 3258 9565 Fax 03 3258 9567 Korea Tel 02 2278 5147 Fax 02 2264 1729 Malaysia Tel 603 5638 9940 Fax 603 5638 9829 Singapore Tel...

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