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MICROMAN E®

 

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USER’S GUIDE

FITTING THE CAPILLARY PISTON

 

MICROMAN E®

Top

1
2

Chapter 3

pIpETTING

Setting the Volume

The volume of liquid to be aspirated is set using 
the volumeter. The volumeter consists of three 
number dials, which are read from top (most 
significant digit) to bottom (least significant digit). 
A marker is used to set exact or intermediate 
volumes using the scale on the bottom dial.
The dials are colored either black or red to indicate 
the position of the decimal point, according to the 
model (see examples).

The volume is set by turning the thumbwheel
slowly to reach the required setting.

For small volume changes

, directly and slowly 

turn the thumbwhell.

To obtain maximum accuracy when 
setting the volume, proceed as 
follows:

 

when 

decreasing

 the volume 

setting, slowly reach the 

required setting, making sure 

not to overshoot the mark.

 

when 

increasin

g the volume 

setting, pass the required value 

by 1/3 of a turn and then slowly 

decrease to reach the volume, 

making sure not to pass the 

setting. 

8

3

6

8

36.8 µL

M50

0

6

6.8 µL

M25

1

6

8

168 µL

M250

0

7

5

0.75 mL

M1000

E

E

E

E

0

6

8

6.8 µL

M10

0

6

8

68 µL

M100

E

E

0

6

8

6.8 µL

M10

0

6

8

68 µL

M100

E

E

8

3

6

8

36.8 µL

M50

0

6

6.8 µL

M25

1

6

8

168 µL

M250

0

7

5

0.75 mL

M1000

E

E

E

E

COLOR OF VOLUMETER NUMBERS

MODEL

BLACK

RED

INCREMENT

M10E, M25E, M50E µL

0.1 µL

0.02 µL

M100E, M250E

µL

none

0.2 µL

M1000E

0.1 and 0.01 mL

mL

2 µL

Summary of Contents for microman e

Page 1: ...User s Guide EN E...

Page 2: ...3 Description 4 Operating Ranges and Material 5 Specifications 6 FITTING THE CAPILLARY PISTON 8 PIPETTING 10 Setting the Volume 10 Aspirating 11 Dispensing 11 Ejecting the Capillary Piston 12 TROUBLE...

Page 3: ...mechanism isolating the aspirated liquid from the pipette body The positive displacement mechanism prevents the sample to sample contamination that can result from the aerosol effect MICROMAN E uses...

Page 4: ...arcode sticker Good Laboratory Practice GLP Features No need to touch disposables capillary piston Serial Number engraved on pipette body Barcode on the box and with the certificate can be transferred...

Page 5: ...icating the volume range for aspirating and dispensing Body or handle Capillary Piston Shaft Thumbwheel for setting the volume Volumeter Volume unlock button Data matrix code 1 2 3 4 5 6 7 8 9 1 2 3 4...

Page 6: ...PP M25E SS Be Alloy PVDF PVDF PP M50E SS Be Alloy PVDF PVDF PP M100E SS Be Alloy PBT PVDF PP M250E SS Be Alloy PVDF PVDF PP M1000E SS Be Alloy PVDF PVDF PP CP CAPILLARY C PISTON P CP10 Polypropylene P...

Page 7: ...tored conditions ISO 8655 6 Basis of adjustment Ex Reference temperature 20 C Relative humidity 50 Barometric pressure 101 kPa Use of distilled water grade 3 ISO 3696 Ten measurements for each test vo...

Page 8: ...3 Max 25 0 30 0 10 0 7 0 3 M50E FD10003 Min 20 0 34 0 20 0 7 0 3 Max 50 0 70 0 30 0 7 0 3 M100E FD10004 Min 10 0 50 0 20 1 5 0 6 50 0 75 0 30 1 5 0 6 Max 100 1 00 0 40 1 5 0 6 M250E FD10005 Min 50 1...

Page 9: ...it a capillary piston 1 Take care not to damage the sealing tip when handling the piston 2 Select a piston and slide it into the capillary 3 Gently push the capillary until it snaps onto the capillary...

Page 10: ...fit a capillary piston 1 Press the MICROMAN E onto the capillary piston until it is firmly seated 2 Pick up the CP from the rack 3 Slowly press the push button until you feel and hear a slight click a...

Page 11: ...setting For small volume changes directly and slowly turn the thumbwhell To obtain maximum accuracy when setting the volume proceed as follows when decreasing the volume setting slowly reach the requ...

Page 12: ...g 1 Press the push button to the first stop 2 Immerse the capillary 2 mm into the liquid 3 Slowly release the push button to draw up the liquid top position 4 Wipe any liquid from the outside of the c...

Page 13: ...apillary against the inside wall of the recipient vessel 1 Press the push button slowly to the first stop 2 Keeping the push button depressed move the capillary away from the sidewall Withdraw MICROMA...

Page 14: ...press the push button to the first stop and then press down harder to the second stop Difficult to fit a capillary Clean the capillary holder with ethanol Without any capillary piston press the push...

Page 15: ...es may be used 2 Wipe the entire pipette with a soft cloth or lint free tissue soaked with distilled water 3 Leave the parts to dry by evaporation or wipe them down with a clean soft cloth or lint fre...

Page 16: ...contaminant you use check with the supplier that the product is compatible with the materials used for the component parts of the pipette and that does not attack any of the following plastics and met...

Page 17: ...or wipe them down according to the instructions provided by the manufacturer or supplier of the decontaminant 5 Rinse the individual components with distilled or sterilized water 6 Leave the parts to...

Page 18: ...t This warranty shall also be void in the event pipettes are altered or modified by any party other than Gilson or its designates Gilson s sole liability under this warranty shall be limited to at Gil...

Page 19: ...Notes 18 MICROMAN E USER S GUIDE NOTES...

Page 20: ...t PO Box 620027 Middleton WI 53562 USA T 608 836 1551 or 800 445 7661 F 608 831 4451 Gilson S A S 19 avenue des Entrepreneurs BP 145 F 95400 Villiers le Bel France T 33 0 1 34 29 50 00 F 33 0 1 34 29...

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