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GE TYPHOON FLA 7000, Getting Started

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GE TYPHOON FLA 7000 Getting Started Download Page 74

5

Reading fluorescent samples

Í þessum kafla

Þessi kafli inniheldur eftirfarandi hluta:

Sjá bls.

hluti

75

5.1 Set the reading mode and reading conditions

77

5.2 Place the sample on the stage

80

5.3 Place the stage in Typhoon FLA 7000

81

5.4 Read the sample

74

Getting Started with Typhoon FLA 7000 28-9607-64 AD

5 Reading fluorescent samples

Summary of Contents for TYPHOON FLA 7000

Page 1: ...Getting Started with Typhoon FLA 7000 tt r ensku...

Page 2: ...00 Control Software for Windows 7 56 3 5 4 Installing 64 bit Typhoon FLA 7000 control software for Windows 7 68 3 6 Preparations before starting Typhoon FLA 7000 69 4 Notkun 70 4 1 Yfirlit um notkun 7...

Page 3: ...8 3 Place the stage in the Typhoon FLA 7000 101 8 4 Read the sample 102 bls A Other settings 104 bls B Daily maintenance 105 bls C Reglulegt vi hald 106 bls D Installing and replacing filters 111 bls...

Page 4: ...Typhoon FLA 7000 system in a safe way Forkr fur Til a starfr kja Typhoon FLA 7000 til tla an h tt ver ur a uppfylla eftirfarandi skilyr i You have read and understood the safety instructions outlined...

Page 5: ...ingar VI V RUN ATHUGI og TIL KYNNING var andi rugga notkun v runnar Sj skilgreiningar a ne an VI V RUN VI V RUN varar vi h ttu standi sem getur leitt til dau a e a alvarlegs slyss ef ekki er fari a me...

Page 6: ...era sem best r gar i bending Feitletrun og tv punktar hugb na aratri i eru au kennd me texta me sk letrun Tv punktur greinir milli repa valmynd annig v sar File Open til Open skipunarinnar File valmyn...

Page 7: ...ts a ila Samr mi vi tilskipanir Evr pusambandsins Vara essi samr mist eim Evr putilskipunum sem eru taldar upp t flunni me v a uppfylla samsvarandi samh f a sta la Titill Tilskipun V latilskipun 2006...

Page 8: ...samsvarandi samr misyfirl sing Evr pusambandsins gilda um t ki egar a er nota sem sj lfst eining e a tengt rum v rum sem er m lt me e a l st notendag gnunum og nota sama r ki og GE afhenti a a undans...

Page 9: ...gin kostna Environmental requirement Titill Krafa Tilskipun um takmarkanir h ttulegum efnum e Restriction of Hazardous Substances RoHS 2011 65 ESB Tilskipun um rgang raf og rafeindab na ar e Waste Ele...

Page 10: ...mW CW 650 nm LD laser class 3B The beam divergence of all laser modules is collimated Efnisyfirlit Samr mi tengdra t kja vi reglur Allur b na ur sem er tengdur Typhoon FLA 7000 skal uppfylla ryggiskr...

Page 11: ...sphor screens Four lasers and multiple filters enable the use of a variety of fluorescent dyes and detection methods Data is aquired through high resolution sensitivity and wide range linearity Compat...

Page 12: ...E F G H I J K L M N O P 1 2 3 4 5 6 7 8 9 Virkni Hluti Power switch located between the AC connector and the USB connector 1 Indicator lamps 2 Knob 3 Lid 4 Setting block 5 Filter change door 6 Filter...

Page 13: ...11 Protection cover for storage phosphor screen 12 Fluor Stage Fluor stage 13 Digitizing fluorescent plate 14 Membrane weight included in Fluor stage 15 Getting Started with Typhoon FLA 7000 28 9607 6...

Page 14: ...Business SP2 32 bit e a Microsoft Windows 7 Professional 32 bit e a Microsoft Windows 7 Professional 64 bit 1 GB Internal memory Intel Core 2 Duo processor Processor 80 GB Hard drive 1280 1024 pixels...

Page 15: ...k to perform digitization 3 Method button click to register or erase a combination of laser and filter 4 Filter Module button click to change or register a filter 5 Preferences button click to set the...

Page 16: ...e file where the image is saved and can be viewed with the analyzing software 3 Method set the method to use in the scan Up to 4 scans can be performed in a row all with individual methods 4 PMT set t...

Page 17: ...he mode for the reading area borders 9 Select Grid to limit the borders to the grid lines Select Free for an arbitrary area Select All to use the entire Fluor stage as the reading area Save Condition...

Page 18: ...kafli inniheldur eftirfarandi hluta Sj bls hluti 19 2 1 ryggisr stafanir 25 2 2 Merkingar 30 2 3 Verklagsreglur ney artilvikum 31 2 4 Uppl singar um endurvinnslu 18 Getting Started with Typhoon FLA 70...

Page 19: ...fire or electric shock Stop using the equipment immediately turn off the power switch and unplug the equipment from the power outlet Contact your local GE representative to request repair VI V RUN Do...

Page 20: ...h as lenses filters or lights Laser safety VI V RUN Never detach the inner cover screwed to this instrument If it is detached laser beam may leak with a risk of loss of vision A V RUN Never cancel the...

Page 21: ...nstructions of your radiation admin istrator Var arr stafanir gegn slysum f lki A V RUN Always wear gloves protective glasses and a lab coat or similar when handling samples A V RUN Always wear cotton...

Page 22: ...oving the unit A V RUN Do not connect any USB devices other than the Typhoon FLA 7000 to the computer in which the Typhoon FLA 7000 Control Software is installed Use only the Typhoon FLA 7000 Control...

Page 23: ...storage phosphor screens in places where the en vironmental radiation may be increased for example rooms with concrete walls or in basements Vi hald VI V RUN Do not attempt to modify the equipment or...

Page 24: ...herwise the equipment may be influenced by electrical nosie A V RUN Turn off the power switch and remove connecting cables before moving the equipment A V RUN Turn the power switch off before cleaning...

Page 25: ...king ar Vi v run Lesi notendauppl singarnar ur en kerfi er nota Fjarl g i ekki hl far af t kinu n skipti um hluti nema notendauppl singarnar heimili a s rstaklega Kerfi samr mist kr fum um rafsegulsvi...

Page 26: ...rk kve inna h ttulegra efna rafeindab na i Labels concerning laser light Hva t kni merkir Merkingar CAUTION Avoid exposure to the laser beam when the lid is open CAUTION Class 3B Laser product when op...

Page 27: ...Labels at the front of Typhoon FLA 7000 Getting Started with Typhoon FLA 7000 28 9607 64 AD 27 2 ryggislei beiningar 2 2 Merkingar...

Page 28: ...Labels at rear of Typhoon FLA 7000 28 Getting Started with Typhoon FLA 7000 28 9607 64 AD 2 ryggislei beiningar 2 2 Merkingar...

Page 29: ...Labels on left side of Typhoon FLA 7000 I Power ON O Power OFF USB I Power ON O Power OFF Getting Started with Typhoon FLA 7000 28 9607 64 AD 29 2 ryggislei beiningar 2 2 Merkingar...

Page 30: ...from the power outlet 3 Rafmagnsleysi The results of a power failure depends on the unit or units affected Results Unit affected by power failure The reading is interrupted immediately The instrument...

Page 31: ...d prevention section of the User Manual If the pollution level exceeds the limit dispose of the instrument body as radioactive waste Otherwise dispose of the materials according to applicable laws and...

Page 32: ...3 3 1 Kr fur um sta setningu og umhverfi t kisins 34 3 2 Transporting Typhoon FLA 7000 35 3 3 Delivery inspection 36 3 4 Tengingar 37 3 5 Uppsetning hugb na ar 68 3 6 Preparations before starting Typh...

Page 33: ...1 5 A Aflgjafi 50 til 60 Hz Line frequency Stable horizontal surface Sta setning Do not place in direct sunlight or in brightly lit places 10 C to 30 C Umhverfishiti 20 to 75 non condensing Raki 2000...

Page 34: ...The Typhoon FLA 7000 weighs approximately 62 kg and requires at least two persons to lift and move Precautions before moving the instrument A ger rep Turn off the Typhoon FLA 7000 1 Turn off the comp...

Page 35: ...g Typhoon FLA 7000 inspect the package for external damages Should you find any external damages notify the delivery company and contact GE for further advice Getting Started with Typhoon FLA 7000 28...

Page 36: ...he USB cable to a USB port on the computer 2 A V RUN Do not connect any USB devices other than the Typhoon FLA 7000 to the computer in which the Typhoon FLA 7000 Control Software is installed Use only...

Page 37: ...ion is performed in the following sequence 1 Install the USB control driver 2 Install the USB function driver Windows XP only 3 Install theTyphoon FLA 7000 control software Getting Started with Typhoo...

Page 38: ...wizard 3 Click the Next button in Add hardware wizard 4 Select Yes I have already connected the hardware and click Next 5 Select Add a new hardware device and click the Next button 6 Select Install t...

Page 39: ...click the Open button 13 Click the OK button in the Install from disk dialog 14 Click the Next button in the Add hardware wizard 15 Click the Next button again 16 Click the Continue Anyway button in...

Page 40: ...d New Hardware Wizard dialog 3 Insert the installation DVD 4 Select Install the software automatically Recommended 5 Click the Next button in the Found New Hardware Wizard dialog 6 Click the Finish bu...

Page 41: ...ation folder in the dialog 5 Click the Next button to install the software at the default folder C Program Files Click the Change button to install to a different folder Click the Install button in th...

Page 42: ...isyfirlit A ger rep Disconnect Typhoon FLA 7000 from the computer 1 Open the control panel and click Classic View in the upper left corner 2 Open Add Hardware 3 In the Add Hardware dialog click the Ne...

Page 43: ...Click the Next button in the wizard Add hardware 13 Click the Next button once again 14 The following warning is displayed Proceed by clicking Install this driver software anyway 15 Click the Finish...

Page 44: ...ent is not acceptable please contact a GE representative See the back cover of this manual for contact information 4 Select I accept the terms in the license agreement and click the Next button Select...

Page 45: ...ish button 9 The installation of Typhoon FLA 7000 is now completed Connect the computer and the Typhoon FLA 7000 with the USB cable 10 Getting Started with Typhoon FLA 7000 28 9607 64 AD 45 3 Uppsetni...

Page 46: ...A ger rep Click Start at the bottom left corner on the screen 1 Click Control Panel System and Security then click Change when the computer sleeps under Power Options 2 In the Put the computer to slee...

Page 47: ...tart at the bottom left corner on the screen 1 Click Control Panel System and Security System to bring up View basic information about your computer window 2 Note the system type displayed in the wind...

Page 48: ...w If the following message is displayed Correction Cause Restart the installation using the installer for Windows 7 with 64 bit operating system The computer is running on Windows 7 with 64 bit operat...

Page 49: ...onfirm your actions in a dialog with the text Windows needs your permission to continue Enter an administrator password if prompted then click Continue to proceed with the installation Efnisyfirlit A...

Page 50: ...dware dialog click Next 5 Select Install the hardware that I manually select from a list Advanced and click Next 6 50 Getting Started with Typhoon FLA 7000 28 9607 64 AD 3 Uppsetning 3 5 Uppsetning hu...

Page 51: ...VD and click Browse 9 Select to install the driver from the Typhoon FLA 7000 DVD 10 Select the USB Control folder and click Open 11 Getting Started with Typhoon FLA 7000 28 9607 64 AD 51 3 Uppsetning...

Page 52: ...dd Hardware wizard 14 Click the Next button once again 15 The following warning is displayed Proceed by clicking Install this driver software anyway 16 52 Getting Started with Typhoon FLA 7000 28 9607...

Page 53: ...on to continue Enter an administrator password if prompted then click Continue to proceed with the installation Efnisyfirlit A ger rep Insert the Typhoon FLA 7000 DVD 1 Locate and double click the fil...

Page 54: ...reement and click the Next button Select the destination folder in the dialog 5 Click the Next button to install the software at the default folder C Program Files Click the Change button to install t...

Page 55: ...ick Install this driver software anyway in the Windows Security dialog 8 Click the Finish button to complete the installation 9 Connect the computer and the Typhoon FLA 7000 with the USB cable 10 Gett...

Page 56: ...as described below A ger rep Click Control Panel System and Security then click Change when the computer sleeps under Power Options 1 Select Never in the drop down menu by Put the computer to sleep th...

Page 57: ...he system type displayed in the window Use the information to choose the appropriate installer 3 Choose the appropriate installer to prevent failure If the installer does not match with the type of th...

Page 58: ...7 with 32 bit operating system You have chosen the installer for Windows 7 with 64 bit operating system Install USB control driver During software installation you may be asked to confirm your actions...

Page 59: ...er Devices and Printers 3 On the Action menu click Add legacy hardware 4 In the Add Hardware dialog click Next 5 Getting Started with Typhoon FLA 7000 28 9607 64 AD 59 3 Uppsetning 3 5 Uppsetning hugb...

Page 60: ...rom a list Advanced and click Next 6 Select Show All Devices and click Next 7 Click Have Disk 8 60 Getting Started with Typhoon FLA 7000 28 9607 64 AD 3 Uppsetning 3 5 Uppsetning hugb na ar 3 5 4 Inst...

Page 61: ...are DVD 2 Click Open 3 10 2 1 3 In the Installer for 64bit USB Control 64 folder click Open 11 Select DevMng64 inf and click Open 12 In Install from disk dialog click OK 13 Getting Started with Typhoo...

Page 62: ...r hardware is displayed click Next 15 When the message Would you like to install this device software is displayed click Install 16 62 Getting Started with Typhoon FLA 7000 28 9607 64 AD 3 Uppsetning...

Page 63: ...dministrator password when prompted then click Continue Efnisyfirlit A ger rep Insert the Typhoon FLA 7000 DVD 1 In the Installer for 64 bit folder locate and double click the file Typhoon FLA 7000 fo...

Page 64: ...licence agreement and click Next 5 Define the software destination folder in the Destination Folder dialog as described below 6 If the default folder C Program Files x86 is acceptable click Next If yo...

Page 65: ...programs wants access to your computer Click Allow 8 When the message Would you like to install this device software is displayed click Install 9 Getting Started with Typhoon FLA 7000 28 9607 64 AD 65...

Page 66: ...Typhoon FLA 7000 A ger rep Click Start at the bottom left corner on the screen 1 Click Control Panel Programs then click Uninstall a program under Programs and Features 2 66 Getting Started with Typho...

Page 67: ...he software Follow the instructions below to upgrade the Typhoon FLA 7000 A ger rep Uninstall the current version of the Typhoon FLA 7000 as described in the section Uninstall the software bls 66 abov...

Page 68: ...puter with a USB cable The air intake fan on the rear side of Typhoon FLA 7000 is unobstructed The air exhaust fan on the rear side of Typhoon FLA 7000 is unobstructed Typhoon FLA 7000 is not placed i...

Page 69: ...la essi kafli inniheldur eftirfarandi hluta Sj bls hluti 70 4 1 Yfirlit um notkun 71 4 2 Starting the instrument and the Typhoon FLA 7000 control software Getting Started with Typhoon FLA 7000 28 9607...

Page 70: ...nd the sample in the Typhoon FLA 7000 Read the sample Phosphorimaging samples Expose the storage phosphor screen Turn on the Typhoon FLA 7000 Start the computer and launch the Typhoon FLA 7000 Control...

Page 71: ...ch on the left side of the body to the I position 1 When the power switch is turned on the indicator lamps change as shown below 2 After turning on the power swich During self diagnosis or startup adj...

Page 72: ...ay deteriorate The scanned data can then not be guaranteed A ger rep Turn on the computer 1 Make sure that Typhoon FLA 7000 has completed the warm up after which only the power lamp on the upper left...

Page 73: ...us messages are as follows Explanation Skilabo Cannot recognize Typhoon FLA 7000 Please check connection and power Disconnected Typhoon FLA 7000 is in self diagnosis Please wait Warm up The unit is re...

Page 74: ...irfarandi hluta Sj bls hluti 75 5 1 Set the reading mode and reading conditions 77 5 2 Place the sample on the stage 80 5 3 Place the stage in Typhoon FLA 7000 81 5 4 Read the sample 74 Getting Starte...

Page 75: ...the fluorescence mode Set the reading conditions by following the steps in the table below All parts in the window are described in Overview of the main window bls 15 Efnisyfirlit 1 2 3 4 7 6 10 11 8...

Page 76: ...pixel size for high quality images Note that a small pixel size setting increases the reading time and the size of the image file Latitude Specify the dynamic range 8 L5 provides a larger detectable...

Page 77: ...e on the Fluor stage Efnisyfirlit The sample must not be thicker than 30 mm For solutions the maxim um height is 4 mm Make sure there are no bubbles or gaps on the contact surface A ger rep Place the...

Page 78: ...ace the titer plate in the desired position in the titer plate frame 2 Placing a gel sample with glass on the Multi stage A ger rep Place a gel sample with glass on the Multi stage 1 78 Getting Starte...

Page 79: ...down the spring loaded glass holders on the glass 2 Tighten the screws on the glass holders as necessary 3 Getting Started with Typhoon FLA 7000 28 9607 64 AD 79 5 Reading fluorescent samples 5 2 Plac...

Page 80: ...e lid 1 Position the stage so that the black triangle mark is aligned with the cor responding mark on the setting block Fit the setting block pin in the hole of the stage 2 Pull the knob up and close...

Page 81: ...rom the location where the reading was stopped Efnisyfirlit Adjust the display parameters Explanation ttur Adjust the gradations in the image Curve Exponential gradation adjustment Exponential Linear...

Page 82: ...fli inniheldur eftirfarandi hluta Sj bls hluti 83 6 1 Handling precautions 85 6 2 Preparing the Storage phosphor screen 87 6 3 Exposing the Storage phosphor screen 82 Getting Started with Typhoon FLA...

Page 83: ...hosphor screen A V RUN Always wear cotton gloves when handling Storage phosphor screens TILKYNNING Use a suction rod when taking the Storage phosphor screen out of the cassette Prying the Storage phos...

Page 84: ...he Storage phosphor screen is reduced Volatile solvents may deform the protective film on the Storage phosphor screen TILKYNNING Protect exposed Storage phosphor screens from light until the reading i...

Page 85: ...the Storage phosphor screen The FLA Image Eraser can erase the Storage phosphor screen in around 15 minutes if it is not overly exposed For details refer to the FLA Image Eraser User Manual Dim the l...

Page 86: ...the time required for X ray film Take this into consideration when setting the initial exposure time Efnisyfirlit For increased image quality minimize the time between exposing the Storage phosphor s...

Page 87: ...screen A tritium storage phosphor screen can be used only once 2 Open the top cover of the cassette Unlock the cassette by sliding the buttons on both sides up 3 Place the sample on the cassette Make...

Page 88: ...le Make sure that the notch of the Storage phosphor screen is in the front left corner of the cassette as shown below 5 Close the cover of the cassette Press on the cover until it clicks into place 6...

Page 89: ...luta Sj bls hluti 90 7 1 Set the reading conditions 93 7 2 Place the storage phosphor screen on the phosphor stage 94 7 3 Place the phosphor stage in the Typhoon FLA 7000 95 7 4 Read the sample Gettin...

Page 90: ...rimaging mode Set the reading conditions by following the steps in the table below All parts in the window are described in Overview of the main window bls 15 Efnisyfirlit 1 2 3 4 5 6 7 8 9 10 11 L si...

Page 91: ...ning area 2 Drag the sides of the red square as needed to adjust the size of the scanning area Pixel Size set the pixel size 7 Choose a small pixel size for high quality images Note that a small pixel...

Page 92: ...ick this button to load previously saved reading conditions if desired For details refer to the User Manual 11 92 Getting Started with Typhoon FLA 7000 28 9607 64 AD 7 Reading phosphorimaging samples...

Page 93: ...phosphor stage 2 A B C D E F G H I J K L M N O P A B C D E F G H I J K L M N O P Pick up the storage phosphor screen and move it to the phosphor stage 3 Place the storage phosphor screen on the back o...

Page 94: ...hat the black triangle mark is aligned with the cor responding mark on the setting block 2 Fit the setting block pin in the hole of the stage 3 Pull the knob up and close the lid 4 94 Getting Started...

Page 95: ...cannot be resurmed from the location where the reading was stopped Efnisyfirlit Adjust the display parameters Explanation ttur Adjust the gradations in the image Curve Exponential gradation adjustmen...

Page 96: ...arandi hluta Sj bls hluti 97 8 1 Set the reading mode and reading conditions 99 8 2 Place the sample on the stage 100 8 3 Place the stage in the Typhoon FLA 7000 101 8 4 Read the sample 96 Getting Sta...

Page 97: ...the digitization mode Set the reading conditions by following the steps in the table below All parts in the window are described in Overview of the main window bls 15 Efnisyfirlit 1 2 3 4 5 6 7 8 9 10...

Page 98: ...increases the reading time and the size of the image file Latitude Specify the dynamic range 8 L5 provides a larger detectable range than L4 L4 provides a finer density gradation if the signals of the...

Page 99: ...olutions the maxim um height is 4 mm Make sure there are no bubbles or gaps on the contact surface A ger rep Place the sample on the Fluor stage 1 Place the fluorescent plate for digitization on top o...

Page 100: ...ith the cor responding mark on the setting block Fit the setting block pin in the hole of the stage 2 Gently place the fluorescent plate for digitizing on top of the digitized sample 3 Pull the knob u...

Page 101: ...d cannot be resurmed from the location where the reading was stopped Efnisyfirlit Adjust the display parameters Explanation ttur Adjust the gradations in the image Curve Exponential gradation adjustme...

Page 102: ...laser Correcti on Mode Manual The correction method must be selected individually in the Reader Settings window Efnisyfirlit The correction mode settings list is registered by a serviceman Please cont...

Page 103: ...l file a read image can also be saved in TIFF file format For TIFF files image data type is always set to Linear format Contains the path to the application used to analyze the data Launch Applicati o...

Page 104: ...the stage from the main body of the instrument 1 Wipe the stage with a sponge moistened with a fluorescence free neutral detergent 2 Thoroughly rinse the stage with water and dry with a lint free clot...

Page 105: ...at least once every 30 days to ex ecute calibration This calibration makes sure that the SHG laser operates properly Failure to calibrate the Typhoon FLA 7000 every 30 days reduces the lifespan of the...

Page 106: ...om the filter module 3 Install the new filter in the filter module 4 Place the filter module in the scanner 5 Register the filter change in the Typhoon FLA 7000 Remove the filter module from the scann...

Page 107: ...r module straight out 3 Remove and install filters in the filter module A ger rep Press the notch on the bottom of the filter module and pull out the filter 1 Getting Started with Typhoon FLA 7000 28...

Page 108: ...he filter module 2 Push the new filter in until it locks in place Place the filter module in the scanner A ger rep Open the filter change door 1 108 Getting Started with Typhoon FLA 7000 28 9607 64 AD...

Page 109: ...filter change door 3 Register the filter change in Typhoon FLA 7000 A ger rep Click the Filter Module button in the main window 1 Click the desired filter position on the filter tray 2 Getting Started...

Page 110: ...t 3 Click the Insert button to register the filter 4 bending You can also click and drag the filter from Filter List to the desired filter position Click the OK button 5 110 Getting Started with Typho...

Page 111: ...0 SYPRO Ruby Y520 SYPRO Orange Y520 FAM Y520 Alexa Fluor 488 Y520 Digitize473 532 nm laser Filter type Method name O580 Cy3 O580 EtBR O580 ROX O580 Pro Q Diamond O580 Digitize532 635 nm laser Filter t...

Page 112: ...r type Method name R670 Alexa Fluor 633 R670 DDAO 650 nm laser Filter type Method name IP Phosphorimaging R710 CBB 112 Getting Started with Typhoon FLA 7000 28 9607 64 AD E Default sample detection me...

Page 113: ...image size Reading mode 20 cm x 40 cm maximum Storage phosphor screen 18 cm x 36 cm valid field angle 24 cm x 40 cm maximum Fluorescent 22 cm x 36 cm valid field angle 238 mm x 398 mm setting size 24...

Page 114: ...7 5 x 15 Storage phosphor screen cassette Approx 2 429 x 283 x 35 Phosphor stage Approx 1 429 x 283 x 35 Fluor stage Approx 1 3 429 x 283 x 33 Multi stage 14 5 603 x 512 x 163 FLA Image Eraser The Rea...

Page 115: ...ghting level to about 20 lux when moving a sample from the cassette onto the Phosphor stage after exposure 20 lux is the brightness level at which most people can read newspaper sized text after he sh...

Page 116: ...sta er seld samr mi vi s luskilm la fyrirt kisins innan GE Healthcare sem selur v runa veitir j nustuna Eintak af skilm lunum er f anlegt samkv mt bei ni Hafi samband vi fulltr a GE Healthcare ykkar s...

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