F
LEXCELL
®
I
NTERNATIONAL
C
ORPORATION
v
FX-5000
Q
UICK
S
TART
I
NSTRUCTIONS
1.
Choose a location for the FX-5000
™
Tension
system that is near the incubator.
2.
Place the computer, monitor, keyboard,
mouse and FX5K
™
Tension FlexLink
®
on a
sturdy, flat surface.
3.
Connect the keyboard, mouse and monitor to
the computer according to the manufacturer’s
instructions.
4.
Connect the FlexLink
®
to the Ethernet port
on the back of the computer using the
Ethernet cable supplied (see Figs. 2-3, p.2).
5.
Connect the computer, monitor and
FlexLink
®
to a power source using a surge
protector.
6.
Remove the white plugs from the four ports
on the back of the FlexLink
®
.
7.
Connect the system vacuum to the
SYSTEM
port on the back of the FlexLink
®
and check
the system vacuum supply (see manual
section 2.6 for pump specifications
recommendations).
8.
Cut two lengths of tubing from those
supplied: cut a 10' (3 m) length of the ¼” (6.4
mm) and cut a 10’ length of the
⅜
” (9.5 mm)
tubing. Connect each length of tubing to the
rear connection of the FlexLink
®
in the
FLEX IN
and
FLEX OUT
ports,
accordingly. Attach provided quick
disconnects to the other end of each piece of
tubing.
9.
Place the
Water Traps (see Fig. 1, p. 2)
in line
with the
FLEX IN
and
FLEX OUT
tubing:
a.
Make cuts in the tubing for the Water
Traps to be positioned as near as possible
to the baseplate, outside of the incubator,
so that the water will be trapped away
from the FlexLink
®
.
b.
Place the Water Traps at a position below
the FlexLink
®
unit.
10.
Place the baseplate(s) in the incubator:
a.
Insert the ¼” (6.4 mm) and
⅜
” (9.5 mm)
tubing into the access opening of the
incubator.
b.
Connect the quick disconnects for the ¼”
(6.4 mm) and
⅜
” (9.5 mm) tubing to the
baseplate(s).
c.
Place four Loading Stations
™
into the
four baseplate wells (optional) and apply
lubricant to the tops of the white buttons
of the loading posts.
d.
Place four gaskets on four BioFlex
plates and place a BioFlex
®
plate and
gasket in each baseplate well.
11.
Turn the FlexLink
®
and computer system
ON. The Windows
desktop should appear
on the monitor. Double click on the FX-
5000
™
icon on the left-hand side of the screen
to start the software.
12.
In the
Configure
menu, select
User Editor
and register as a user.
13.
In the
Regimen
menu, select
Create new
and
create a regimen by entering values into the
appropriate spaces or click on the
Run
Wizard
button in the bottom left-hand corner
for a step-by-step procedure. When
completed, click on
Save Regimen
. Exit the
regimen editor.
14.
In
Controllers
menu, select the appropriate
FlexLink
®
number to which the regimen will
be downloaded.
15.
In the
Regimen
menu, select
Assign.
Choose
the proper platform, user, and regimen
(see
section 3.7 for assignment instructions)
from the drop-down menus. Confirm with a
Yes
to download the regimen.
16.
Once the program is downloaded, click on
Simulate
in the main window display. Once
satisfied that the regimen is correct, click on
Stop
.
17.
Turn ON the vacuum system. Click on
Start
to run the regimen. The plates should begin
flexing. Make a visual check to verify
membrane movement.
18.
To pause or stop the regimen, click on
Pause
or
Stop
at any time. Otherwise, the program
will stop when the regimen is completed.
