flexcell FX-5000 User Manual Download Page 24

 

F

LEXCELL

®

 

I

NTERNATIONAL 

C

ORPORATION

 

 

 

18

amount (2% elongation or greater). The 
wave may also be somewhat choppy because 
of the low system volume. If the outputs were 
originally only slightly lower or higher than 
the inputs, the problem is likely not a leak 
but calibration. Call Flexcell

®

 for further 

instructions. If the output max % elongation 
increases significantly when the quick 
disconnects are connected together, the leak 
is most likely in the baseplate or gaskets. 

 

Solution 1: 

Remove gaskets and plates from 

baseplate. Check to see that each gasket is 
firmly seated in each plate without any folds 
in the thin bottom lip of the gasket and with 
each inside corner butted up against each 
outside corner of the BioFlex

®

 plate. Place 

each plate and gasket carefully into each 
baseplate well. Check to be sure that the 
gaskets are flatly seated in the wells; i.e., none 
of the sides touching the baseplate to the point 
that the gasket is not able to fully enter the 
well. Move each gasket forward and back and 
side to side within the well to be sure that they 
are somewhat loose in the plane of the 
baseplate. This will allow the vacuum 
pressure to pull the gaskets and plates firmly 
down to seal flat against the baseplate surface. 
Run a regimen. The plates and gaskets may 
require some initial hand pressure to start the 
seal. Place the Plexiglas cover and a couple of 
lead weights (approx. 10 lbs total; variable) on 
top of the plates. If there is still a noticeable 
leak, check all sides of each BioFlex

®

 plate to 

see if the membrane is protruding from any 
side. If there is any noticeable membrane 
protrusion, remove this with a razor blade. 

Problem 2: Regimen wave is flatlining 

 

Solution 2:   

Remove the 

SYSTEM

 vacuum line at the 

back of the FlexLink

®

 and check to be sure 

that there is vacuum coming through the line. 
Check the 

VENT

 port at the back of the 

FlexLink

®

 when the system is running to see 

if air is moving in or out. If not, it is possible 

that the valves are locked due to residue 
buildup. Try applying positive pressure to 
the 

VENT

 port to free the valves.  

If the regimen wave is flatlining but the 
plates are still cycling air pressure, try the 
following: 
 
Disconnect the blue 1/4" (6.4mm) 

FLEX IN

 

tubing from the back of the unit and place a 
finger over the end of the tubing while a 
regimen is running. If air is cycling at the 
end of the tubing then the problem is with 
the FX5K

 Tension FlexLink

®

. Contact 

Flexcell

®

 International for further 

instructions.  
 

Problem 3: Regimen wave is fluttering or 
not smooth 

 

Solution 3: 

This problem could be due to water in 

the valves or the system being out of 
calibration. Run the drying regimen with the 
drying filter (as described in the manual) to 
dry the system and valves. If this does not 
resolve the problem try running the drying 
regimen once more. If the problem 
continues, contact Flexcell

®

 for further 

instructions. 
 

Problem 4: Programming a long regimen 
(1 day or more) but the software will not 
allow the regimen to be saved. 

 

Solution 4:  

The software has a limit on the number 

of cycles that can be entered into any given 
step (65,535). If a regimen is programmed 
with a step that requires more than 65,535 
cycles, the software will give an "invalid 
entry" error. To run the regimen for the time 
required additional steps will need to be 
programmed. This will not create any breaks 
in the regimen. Simply divide the total time 
in half (or as many times as is necessary) and 
make additional steps with the same 
parameters. See section 3.5 of the manual for 

Summary of Contents for FX-5000

Page 1: ...21 0 Culturing Cells in a Mechanically Active Environment Flexcell International Corporation 2730 Tucker Street Suite 200 Burlington NC 27215 800 728 3714 919 732 1591 FAX 919 732 5196 www flexcellin...

Page 2: ...uctions 7 3 1 Starting the FX 5000 Program 7 3 2 Using the FX 5000 Tutorial 7 3 3 Main Display Window 7 3 4 Configuring Users 9 3 5 Creating Regimens 10 3 6 Creating a Regimen with a Custom Waveform S...

Page 3: ...than our factory or distributor Breaking the seal and opening the unit case or violating unit components will void the warranty Failure to run the drying regimen as recommended in this manual will vo...

Page 4: ...nty are listed below You will need to have the service tag number and Express Service Code number which are located on the side of the computer Dell Hardware Limited Warranty Plus Onsite Service Exten...

Page 5: ...to a properly grounded power outlet and used with the power cord supplied with the unit s unmodified 3 The unit must be operated with the factory installed cover in place 4 No modifications of the equ...

Page 6: ...seplate s in the incubator a Insert the 6 4 mm and 9 5 mm tubing into the access opening of the incubator b Connect the quick disconnects for the 6 4 mm and 9 5 mm tubing to the baseplate s c Place fo...

Page 7: ...D for vacuum source connection 16 Flexcell FX 5000 Tension System user s manual and other ancillary manuals for computer and monitor 17 Surge protected outlet strip 18 Water Trap x2 19 Vacuum source o...

Page 8: ...the system is 48 37 lbs 21 96 kg Figure 3 Dell OptiPlex rear view 2 3 SETUP OF THE COMPUTER AND FX5K TENSION FLEXLINK Setup the Dell OptiPlex desktop computer and peripherals according to the manufac...

Page 9: ...onto the fitting Tighten each nut by hand until the tubing is crimped to minimize vacuum leakage Connect the power cables 115 VAC or 230 VAC to the rear power inlet FlexLink Power of the FlexLink Fig...

Page 10: ...ure that the gaskets are fully pressed onto the culture plates and that no parts are folded under 4 Place the culture plates and gaskets into the baseplate wells Check to be sure that the gaskets are...

Page 11: ...use the auto control pump feature plug the vacuum pump power cable into the PUMP CONTROL CONNECTION outlet on the rear of the FX5K Tension FlexLink Fig 2 and 7 NOTE When using the auto pump control fe...

Page 12: ...imen Ready User name Platform Regimen Name Flexcell International Corp www flexcellint com Regimen is running or being simulated 1 Regimen Left 5 min User name Platform Regimen Name Flexcell Internati...

Page 13: ...ength as much as possible between the vacuum source and the baseplate See page 16 for more information on obtaining the optimal performance of your FX 5000 Tension System 3 1 STARTING THE FX 5000 PROG...

Page 14: ...al data patterns may be confounded with noise For the case of the pressure plot as a function of voltage from the FX 5000 Tension System pressure transducer the rough data yield a plot with a coeffici...

Page 15: ...changed by clicking on one of the two small double arrow buttons at either end of the axis The button with arrows pointing away from each other increases the axis scale while the button with arrows po...

Page 16: ...boxes under Min and Max 4 Enter the frequency for the waveform 0 01 5 Hz in the box under Freq 5 If a static regimen is required enter the desired elongation at which the membrane is to be held in the...

Page 17: ...ed regimen name in the Regimens for Selected User box and then click on Delete Regimen Confirm the deletion by clicking on Yes NOTE If the regimen to be deleted is currently assigned the regimen must...

Page 18: ...ll be MAXIMUM and the regimen cannot be saved Adjust the time and or cycle number to decrease the total duration time 7 Click on Save Regimen 8 When finished with regimen editing click on Close 3 7 AS...

Page 19: ...unusual elements is to accommodate for the lag time during sudden changes in pressure e g rising falling edge of a square wave Without these unusual elements the valve controllers may oscillate for a...

Page 20: ...l turn the pump ON The software will turn the pump OFF when a regimen is complete This option also allows the pump to be turned OFF during a regimen step when a static waveform regimen is selected tha...

Page 21: ...box can be set so that the data viewed in the plot begin at the specified time entered here Use the timeline at the top of the graph to navigate through the regimen Users can move the slider function...

Page 22: ...the incubator and stored flat to prevent warping Do not hang the red gaskets on pegs to dry They will stretch over time 3 18 EXITING THE FX 5000 PROGRAM To exit the FX 5000 software in the Controller...

Page 23: ...elongation and frequency a Shorten the FLEX IN FLEX OUT and SYSTEM tubing to their minimum possible lengths See section 2 2 for minimum lengths b Use a more powerful vacuum source if recommended spec...

Page 24: ...Regimen wave is flatlining Solution 2 Remove the SYSTEM vacuum line at the back of the FlexLink and check to be sure that there is vacuum coming through the line Check the VENT port at the back of the...

Page 25: ...of the cells in the middle of the well due to gravity or b incomplete trypsinization or over trypsinization of cells causing clumping and uneven distribution of cells Solution 8 a Use 2 3 ml of media...

Page 26: ...y dry before connecting to the FX5K Tension FlexLink Contact time time surface remains wet of the above solutions is critical to the effectiveness of disinfecting Times given are minimal and longer co...

Page 27: ...re from min to max Triangle Wave This waveform provides a linear ramped rise to the max and a linear ramped fall back to the min Square Wave This waveform provides the fastest possible rise to max fol...

Page 28: ...inusoidal wave Heart wave Electronic and Pressure Triangular wave Square wave and custom waveforms Provides equibiaxial strain or unconstrained distension to the BioFlex culture plate membrane Optiona...

Page 29: ...m design vacuum controller board o Microprocessor sampling rate for display data transducer 200 Hz o Microprocessor sampling rate for feedback error valve adjustment transducer 1 KHz 2 proportional so...

Page 30: ...equibiaxial strain Minimum achievable elongation with the FX 5000 1 2 Maximum achievable elongation with the FX 5000 8 0 FX 5000 TENSION PHYSICAL SPECIFICATIONS FX 5000 Computer Size W x H x D 3 65 x...

Page 31: ...ion 20 Frequency 1 Hz The maximum elongation achieved might only be 10 Reducing the frequency of the regimen to 0 5 Hz will produce an achievable elongation of 18 The maximum allowable elongation for...

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