flexcell FX-5000 User Manual Download Page 17

 

F

LEXCELL

®

 

I

NTERNATIONAL 

C

ORPORATION

 

 

 

11

9)  When the regimen step is complete, click 

on 

Add Step

 at the bottom. Every time a 

new/edited step is saved, the overall 
duration of the regimen will be calculated 
and displayed in the box labeled 

Duration

 

next to the 

Step

 number box. If the total 

duration exceeds the maximum allowable 
duration, this value will be 

MAXIMUM

and the regimen cannot be saved. Adjust 
the time and/or cycle number to decrease 
the total duration time. 

 
10) To add other steps to a regimen, click in 

the box labeled 

Step

 and type in the next 

step number.  

 
  For example, the first step will 

automatically be Step 1. To add another 
step, delete the 1 and replace it with a 2. 
After this is completed, change the 
parameters as needed for the new step 
according to steps 2 to 9 above. 

 
11) To delete a step that has already been 

added, click on the step in the window 
labeled 

Regimen Steps

, which contains 

the list of steps. This will cause the step to 
be highlighted. Then click on 

Remove 

Step

 at the bottom of the window. 

Confirm the deletion by clicking on 

Yes

 

when prompted. 

 
12) After all steps and parameters have been 

entered, click on 

Save Regimen

 
13) To delete a regimen, simply double-click 

on the desired regimen name in the 

Regimens for Selected User

 box, and 

then click on 

Delete Regimen

. Confirm 

the deletion by clicking on 

Yes

.  

 

NOTE:

 If the regimen to be deleted is currently 

assigned, the regimen must be removed from 
assignment before deleting. 

 
14) When finished with regimen editing, click 

on 

Close

3.6

 

 C

REATING A 

R

EGIMEN WITH A 

C

USTOM 

W

AVEFORM 

S

HAPE

 

 
In the top row of an Excel spreadsheet, enter 
the % elongation values for the desired 
waveform shape for one cycle. Enter one 
value per cell. Values should be chosen 
along a curve at even time intervals, i.e. 
every 100 msec (milliseconds). Save this 
spreadsheet as a .csv file (comma delimited 
file format) in the C:/FlexSyst directory. 

 

Example Custom waveform shape:  Ramp 
waveform shape 
 
Example Excel file:  Ramp waveform shape 

1 1.

5

2 2.

5

3 3.

4 4.

5 5.

5

0

 
 

Example CSV file viewed in Notepad:  Ramp 
waveform shape  

1,1.5,2,2.5,3,3.5,4,4.5,5,5.5,0 
 
In the 

Regimen

 menu of the FX-5000

 

software, select 

Create new

. A new window 

will appear entitled 

Regimens

 with a list of 

the existing users and their regimens. In the 

Existing Users

 box, click on the user name 

under which the regimen is to be created. A 
list of current regimens created under this 
user will appear in the 

Regimens for 

Selected User

 box, unless no regimens have 

been saved under this user in which case, this 
box will be empty. 
 

0

1

2

3

4

5

6

0

100

200

300

400

500

600

700

800

900

1000

Time (msec)

% Elongat

ion

Summary of Contents for FX-5000

Page 1: ...21 0 Culturing Cells in a Mechanically Active Environment Flexcell International Corporation 2730 Tucker Street Suite 200 Burlington NC 27215 800 728 3714 919 732 1591 FAX 919 732 5196 www flexcellin...

Page 2: ...uctions 7 3 1 Starting the FX 5000 Program 7 3 2 Using the FX 5000 Tutorial 7 3 3 Main Display Window 7 3 4 Configuring Users 9 3 5 Creating Regimens 10 3 6 Creating a Regimen with a Custom Waveform S...

Page 3: ...than our factory or distributor Breaking the seal and opening the unit case or violating unit components will void the warranty Failure to run the drying regimen as recommended in this manual will vo...

Page 4: ...nty are listed below You will need to have the service tag number and Express Service Code number which are located on the side of the computer Dell Hardware Limited Warranty Plus Onsite Service Exten...

Page 5: ...to a properly grounded power outlet and used with the power cord supplied with the unit s unmodified 3 The unit must be operated with the factory installed cover in place 4 No modifications of the equ...

Page 6: ...seplate s in the incubator a Insert the 6 4 mm and 9 5 mm tubing into the access opening of the incubator b Connect the quick disconnects for the 6 4 mm and 9 5 mm tubing to the baseplate s c Place fo...

Page 7: ...D for vacuum source connection 16 Flexcell FX 5000 Tension System user s manual and other ancillary manuals for computer and monitor 17 Surge protected outlet strip 18 Water Trap x2 19 Vacuum source o...

Page 8: ...the system is 48 37 lbs 21 96 kg Figure 3 Dell OptiPlex rear view 2 3 SETUP OF THE COMPUTER AND FX5K TENSION FLEXLINK Setup the Dell OptiPlex desktop computer and peripherals according to the manufac...

Page 9: ...onto the fitting Tighten each nut by hand until the tubing is crimped to minimize vacuum leakage Connect the power cables 115 VAC or 230 VAC to the rear power inlet FlexLink Power of the FlexLink Fig...

Page 10: ...ure that the gaskets are fully pressed onto the culture plates and that no parts are folded under 4 Place the culture plates and gaskets into the baseplate wells Check to be sure that the gaskets are...

Page 11: ...use the auto control pump feature plug the vacuum pump power cable into the PUMP CONTROL CONNECTION outlet on the rear of the FX5K Tension FlexLink Fig 2 and 7 NOTE When using the auto pump control fe...

Page 12: ...imen Ready User name Platform Regimen Name Flexcell International Corp www flexcellint com Regimen is running or being simulated 1 Regimen Left 5 min User name Platform Regimen Name Flexcell Internati...

Page 13: ...ength as much as possible between the vacuum source and the baseplate See page 16 for more information on obtaining the optimal performance of your FX 5000 Tension System 3 1 STARTING THE FX 5000 PROG...

Page 14: ...al data patterns may be confounded with noise For the case of the pressure plot as a function of voltage from the FX 5000 Tension System pressure transducer the rough data yield a plot with a coeffici...

Page 15: ...changed by clicking on one of the two small double arrow buttons at either end of the axis The button with arrows pointing away from each other increases the axis scale while the button with arrows po...

Page 16: ...boxes under Min and Max 4 Enter the frequency for the waveform 0 01 5 Hz in the box under Freq 5 If a static regimen is required enter the desired elongation at which the membrane is to be held in the...

Page 17: ...ed regimen name in the Regimens for Selected User box and then click on Delete Regimen Confirm the deletion by clicking on Yes NOTE If the regimen to be deleted is currently assigned the regimen must...

Page 18: ...ll be MAXIMUM and the regimen cannot be saved Adjust the time and or cycle number to decrease the total duration time 7 Click on Save Regimen 8 When finished with regimen editing click on Close 3 7 AS...

Page 19: ...unusual elements is to accommodate for the lag time during sudden changes in pressure e g rising falling edge of a square wave Without these unusual elements the valve controllers may oscillate for a...

Page 20: ...l turn the pump ON The software will turn the pump OFF when a regimen is complete This option also allows the pump to be turned OFF during a regimen step when a static waveform regimen is selected tha...

Page 21: ...box can be set so that the data viewed in the plot begin at the specified time entered here Use the timeline at the top of the graph to navigate through the regimen Users can move the slider function...

Page 22: ...the incubator and stored flat to prevent warping Do not hang the red gaskets on pegs to dry They will stretch over time 3 18 EXITING THE FX 5000 PROGRAM To exit the FX 5000 software in the Controller...

Page 23: ...elongation and frequency a Shorten the FLEX IN FLEX OUT and SYSTEM tubing to their minimum possible lengths See section 2 2 for minimum lengths b Use a more powerful vacuum source if recommended spec...

Page 24: ...Regimen wave is flatlining Solution 2 Remove the SYSTEM vacuum line at the back of the FlexLink and check to be sure that there is vacuum coming through the line Check the VENT port at the back of the...

Page 25: ...of the cells in the middle of the well due to gravity or b incomplete trypsinization or over trypsinization of cells causing clumping and uneven distribution of cells Solution 8 a Use 2 3 ml of media...

Page 26: ...y dry before connecting to the FX5K Tension FlexLink Contact time time surface remains wet of the above solutions is critical to the effectiveness of disinfecting Times given are minimal and longer co...

Page 27: ...re from min to max Triangle Wave This waveform provides a linear ramped rise to the max and a linear ramped fall back to the min Square Wave This waveform provides the fastest possible rise to max fol...

Page 28: ...inusoidal wave Heart wave Electronic and Pressure Triangular wave Square wave and custom waveforms Provides equibiaxial strain or unconstrained distension to the BioFlex culture plate membrane Optiona...

Page 29: ...m design vacuum controller board o Microprocessor sampling rate for display data transducer 200 Hz o Microprocessor sampling rate for feedback error valve adjustment transducer 1 KHz 2 proportional so...

Page 30: ...equibiaxial strain Minimum achievable elongation with the FX 5000 1 2 Maximum achievable elongation with the FX 5000 8 0 FX 5000 TENSION PHYSICAL SPECIFICATIONS FX 5000 Computer Size W x H x D 3 65 x...

Page 31: ...ion 20 Frequency 1 Hz The maximum elongation achieved might only be 10 Reducing the frequency of the regimen to 0 5 Hz will produce an achievable elongation of 18 The maximum allowable elongation for...

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