flexcell FX-5000 User Manual Download Page 19

 

F

LEXCELL

®

 

I

NTERNATIONAL 

C

ORPORATION

 

 

 

13

not be able to run. In which case, click 

Reset 

in the FlexLink

®

 window, and then assign the 

desired regimen again. 
 

3.8

 

 S

TARTING 

R

EGIMENS

 

 
To run an assigned regimen, click on 

Start

 in 

the main display window (Fig. 10) after the 
regimen has finished downloading. Be sure 
that the vacuum pump is ON and all other 
connections are made. The membranes should 
begin flexing and the assigned regimen will be 
in progress. 
 
The progress of the regimen for the currently 
selected FlexLink

®

 will be monitored 

continuously. The displays for Run Time, 
Step #, Cycle #, Jump #, and Min and Max % 
will update, as will the 

actual waveform data

 

returned by the FlexLink

®

. Additionally, a 

progress bar below the graph will display the 
percentage of the regimen that has been 
completed. 
 

3.9  S

IMULATING 

R

EGIMENS

 

 
The 

Simulate

 button is used to simulate a 

regimen graphically without actually running 
it. Simulating a regimen will give the user an 
idea of the waveform output before actually 
running the regimen. To start a simulation, 
click on 

Simulate

 in the main display 

window. A waveform will begin moving 
across the graphic display. This waveform 
corresponds to the ideal waveform as 
generated by the FX-5000

 software, and is 

the actual waveform pattern sent to the 
FlexLink

®

 during the download process. For 

certain waveforms (e.g. square waves), the 
idealized pattern may appear to have some 
unusual elements (e.g. rounded corners). The 
purpose of these unusual elements is to 
accommodate for the lag time during sudden 
changes in pressure (e.g. rising/falling edge of 
a square wave). Without these unusual 
elements, the valve controllers may oscillate 

for a period of time (e.g. dampened sine 
wave during beginning of plateau in a square 
waveform). 
 

3.10

 

 P

AUSING 

R

EGIMENS

 

 
To pause a simulated or running regimen, 
click on 

Pause

 in the main display window. 

Click on 

Yes

 when the program asks for 

confirmation. Pausing will cause the current 
simulation or actual running of the regimen 
to stop at the next cycle of the current 
regimen step. Pausing is different from 
stopping, in that pausing leaves the regimen 
at its current position, whereas stopping 
causes the regimen position to be set to the 
beginning of the first regimen step. 
 

3.11

 

 R

ESUMING 

R

EGIMENS

 

 
To resume a paused regimen, click on 

Resume

 in the main display window. This 

function is available only when a simulated 
or running regimen has been paused. The 
regimen will restart its prior activity 
(simulation or actual run) from its last point. 
 

3.12

 

 S

TOPPING 

R

EGIMENS

 

 
To stop a simulated or running regimen, 
click on 

Stop

 in the main display window. 

Click on 

Yes

 when the program asks for 

confirmation. Stopping causes the current 
activity to cease immediately, and sets the 
regimen position back to the beginning of 
the first regimen step. Stopping is different 
from pausing, in that pausing leaves the 
regimen at its current position so that it can 
be resumed from where it stopped. 
 

3.13

 

 D

ETERMINE THE 

P

ROGRESS OF 

T

HE 

R

EGIMEN

 

 
The progress of the regimen for the currently 
selected FlexLink

®

 will be monitored 

continuously. The displays for 

Run Time

Summary of Contents for FX-5000

Page 1: ...21 0 Culturing Cells in a Mechanically Active Environment Flexcell International Corporation 2730 Tucker Street Suite 200 Burlington NC 27215 800 728 3714 919 732 1591 FAX 919 732 5196 www flexcellin...

Page 2: ...uctions 7 3 1 Starting the FX 5000 Program 7 3 2 Using the FX 5000 Tutorial 7 3 3 Main Display Window 7 3 4 Configuring Users 9 3 5 Creating Regimens 10 3 6 Creating a Regimen with a Custom Waveform S...

Page 3: ...than our factory or distributor Breaking the seal and opening the unit case or violating unit components will void the warranty Failure to run the drying regimen as recommended in this manual will vo...

Page 4: ...nty are listed below You will need to have the service tag number and Express Service Code number which are located on the side of the computer Dell Hardware Limited Warranty Plus Onsite Service Exten...

Page 5: ...to a properly grounded power outlet and used with the power cord supplied with the unit s unmodified 3 The unit must be operated with the factory installed cover in place 4 No modifications of the equ...

Page 6: ...seplate s in the incubator a Insert the 6 4 mm and 9 5 mm tubing into the access opening of the incubator b Connect the quick disconnects for the 6 4 mm and 9 5 mm tubing to the baseplate s c Place fo...

Page 7: ...D for vacuum source connection 16 Flexcell FX 5000 Tension System user s manual and other ancillary manuals for computer and monitor 17 Surge protected outlet strip 18 Water Trap x2 19 Vacuum source o...

Page 8: ...the system is 48 37 lbs 21 96 kg Figure 3 Dell OptiPlex rear view 2 3 SETUP OF THE COMPUTER AND FX5K TENSION FLEXLINK Setup the Dell OptiPlex desktop computer and peripherals according to the manufac...

Page 9: ...onto the fitting Tighten each nut by hand until the tubing is crimped to minimize vacuum leakage Connect the power cables 115 VAC or 230 VAC to the rear power inlet FlexLink Power of the FlexLink Fig...

Page 10: ...ure that the gaskets are fully pressed onto the culture plates and that no parts are folded under 4 Place the culture plates and gaskets into the baseplate wells Check to be sure that the gaskets are...

Page 11: ...use the auto control pump feature plug the vacuum pump power cable into the PUMP CONTROL CONNECTION outlet on the rear of the FX5K Tension FlexLink Fig 2 and 7 NOTE When using the auto pump control fe...

Page 12: ...imen Ready User name Platform Regimen Name Flexcell International Corp www flexcellint com Regimen is running or being simulated 1 Regimen Left 5 min User name Platform Regimen Name Flexcell Internati...

Page 13: ...ength as much as possible between the vacuum source and the baseplate See page 16 for more information on obtaining the optimal performance of your FX 5000 Tension System 3 1 STARTING THE FX 5000 PROG...

Page 14: ...al data patterns may be confounded with noise For the case of the pressure plot as a function of voltage from the FX 5000 Tension System pressure transducer the rough data yield a plot with a coeffici...

Page 15: ...changed by clicking on one of the two small double arrow buttons at either end of the axis The button with arrows pointing away from each other increases the axis scale while the button with arrows po...

Page 16: ...boxes under Min and Max 4 Enter the frequency for the waveform 0 01 5 Hz in the box under Freq 5 If a static regimen is required enter the desired elongation at which the membrane is to be held in the...

Page 17: ...ed regimen name in the Regimens for Selected User box and then click on Delete Regimen Confirm the deletion by clicking on Yes NOTE If the regimen to be deleted is currently assigned the regimen must...

Page 18: ...ll be MAXIMUM and the regimen cannot be saved Adjust the time and or cycle number to decrease the total duration time 7 Click on Save Regimen 8 When finished with regimen editing click on Close 3 7 AS...

Page 19: ...unusual elements is to accommodate for the lag time during sudden changes in pressure e g rising falling edge of a square wave Without these unusual elements the valve controllers may oscillate for a...

Page 20: ...l turn the pump ON The software will turn the pump OFF when a regimen is complete This option also allows the pump to be turned OFF during a regimen step when a static waveform regimen is selected tha...

Page 21: ...box can be set so that the data viewed in the plot begin at the specified time entered here Use the timeline at the top of the graph to navigate through the regimen Users can move the slider function...

Page 22: ...the incubator and stored flat to prevent warping Do not hang the red gaskets on pegs to dry They will stretch over time 3 18 EXITING THE FX 5000 PROGRAM To exit the FX 5000 software in the Controller...

Page 23: ...elongation and frequency a Shorten the FLEX IN FLEX OUT and SYSTEM tubing to their minimum possible lengths See section 2 2 for minimum lengths b Use a more powerful vacuum source if recommended spec...

Page 24: ...Regimen wave is flatlining Solution 2 Remove the SYSTEM vacuum line at the back of the FlexLink and check to be sure that there is vacuum coming through the line Check the VENT port at the back of the...

Page 25: ...of the cells in the middle of the well due to gravity or b incomplete trypsinization or over trypsinization of cells causing clumping and uneven distribution of cells Solution 8 a Use 2 3 ml of media...

Page 26: ...y dry before connecting to the FX5K Tension FlexLink Contact time time surface remains wet of the above solutions is critical to the effectiveness of disinfecting Times given are minimal and longer co...

Page 27: ...re from min to max Triangle Wave This waveform provides a linear ramped rise to the max and a linear ramped fall back to the min Square Wave This waveform provides the fastest possible rise to max fol...

Page 28: ...inusoidal wave Heart wave Electronic and Pressure Triangular wave Square wave and custom waveforms Provides equibiaxial strain or unconstrained distension to the BioFlex culture plate membrane Optiona...

Page 29: ...m design vacuum controller board o Microprocessor sampling rate for display data transducer 200 Hz o Microprocessor sampling rate for feedback error valve adjustment transducer 1 KHz 2 proportional so...

Page 30: ...equibiaxial strain Minimum achievable elongation with the FX 5000 1 2 Maximum achievable elongation with the FX 5000 8 0 FX 5000 TENSION PHYSICAL SPECIFICATIONS FX 5000 Computer Size W x H x D 3 65 x...

Page 31: ...ion 20 Frequency 1 Hz The maximum elongation achieved might only be 10 Reducing the frequency of the regimen to 0 5 Hz will produce an achievable elongation of 18 The maximum allowable elongation for...

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