Etaluma LS400 Startup Manual Download Page 9

Lumascope 500/400 Startup Guide 

 

 

 

 

 

Etaluma, Inc.  |  3129 Tiger Run Court, Suite 112  | Carlsbad, CA 92010  | 760-298-2355  |  www.etaluma.com 

 

 

2.

 

At the end of the AviSynth Script line near the top, click browse (“…”).  A MeGUI window named Open 
AviSynth script will open.  Depending on the Windows version used, the Destination Folder from the 
most recent time-lapse capture may be listed.  Or it may be necessary to browse to your Destination 
Folder.  If your time-lapse included both fluorescent and brightfield images, your Destination Folder will 
contain 2 subfolders, each with the images and corresponding .avs file for that channel.  Open the 
desired folder or subfolder and click on the Avisynth (.avs) file. 

 
3.

 

A preview video window will open; click Play to see a short segment of your video. 

 
4.

 

Return to the MeGUI window and select File Format (MP4 recommended).  Click Queue button for 
Video encoding on right (button about halfway down the window). 

 
5.

 

An encoding window will open and you will see progress in a green horizontal bar until video is 
completed and window closes.  Close MeGUI window. 

 
6.

 

Video will be saved the Destination Folder or the channel subfolder within the Destination Folder 
chosen in Section II.H.6 above. 

 
J.  Recording live videos 

 

 

1.

 

First download and install the AviSynth and MeGUI programs as described in Section II.H.1 above.  

 
2.

 

Click red circle icon in top tool bar of Live Image Window to open Video Configuration dialog box.   

 
3.

 

Choose Filename Root, File Format (TIFF recommended), Destination Folder and if time-stamp is 
desired.  Click OK and images will start to record.  

  
4.

 

Click red circle icon again to stop.  A dialog box will open stating that your images have been saved to 
the Destination Folder (and how to proceed as also listed below).  The Destination folder will also 
contain an Avisynth (.avs) file containing a compiled script of the captured images. 

 
5.

 

To convert the captured images to video, open the MeGUI program (may take several minutes). 

 
6.

 

At the end of the AviSynth Script line, click browse (“…”).  A MeGUI window named Open AviSynth 
script will open.  Browse to your Destination Folder and select the Avisynth (.avs) file. 

 

7.

 

Click Open.  A preview video window will open; click Play to see a short segment of your video. 

 
8.

 

Return to the MeGUI window and select File Format (MP4 recommended).  Click Queue button for 
Video encoding on right (button about halfway down the window). 

 
9.

 

An encoding window will open and you will see progress in a green horizontal bar until video is 
completed and window closes.  Close MeGUI window. 

 
10.

 

Video will be saved in the Destination Folder chosen in Section II.J.3 above. 
 

 

Summary of Contents for LS400

Page 1: ...r assistance please call 760 298 2355 or email support etaluma com Etaluma Inc 3129 Tiger Run Court Suite 112 info etaluma com Carlsbad CA 92010 www etaluma com 760 298 2355 Lumascope and Lumaview are trademarks of Etaluma Inc 2009 2015 Etaluma Inc All rights reserved Lumascope 500 with optional Manual XY Stage Lumascope 500 400 Startup Guide For Use with LS500 LS400 ...

Page 2: ...ectives D First step Installing Lumaview 500 400 software on your computer 1 LS500 and LS400 are controlled by the Lumaview 500 400 software program The latest Lumaview version is downloadable from Etaluma s website and must be installed prior to connecting your computer for the first time to the Lumascope due to the need for a Driver that is installed at the same time For a listing of versions an...

Page 3: ...move the LS500 from its packaging Insert the standard USB A end of the supplied USB power communication cable into a USB port on your computer and the other square USB B end into the square port middle of the three ports on the back of the LS500 see Fig 1 We highly recommend that you use the same USB port on your computer every time you connect to the LS500 It is also best to connect the LS500 dir...

Page 4: ...ctives the optional Objective Removal Tool can also be used to change objectives To use the Objective Removal Tool lower it grip end up through the center hole on the LS500 deck and match the inner notches of the Tool with the notched pattern on the installed Meiji objective body Unscrew the objective and then lower and rotate the Removal Tool just slightly so the notches no longer line up This wi...

Page 5: ...have this version you should download and install the latest version of Lumaview 500 400 see Section I E above 3 It you must use the older version when you left click your mouse or trackpad either a Save As dialog box will open or you will hear a clicking sound indicating that the camera has auto saved an image 4 Read Section II G on capturing images below You can either uncheck the AutoSave funct...

Page 6: ...as one of the 3 listed in Section D 2 above is needed Connecting one of these 3 light sources to the LS500 allows it to be controlled by Lumaview 500 400 To mount the light source above the Lumascope deck see Section D 3 above 5 To illuminate the sample when using ambient light move cursor over Live Image Window right click and select Exposure Settings to open its dialog box Check Auto Gain to mos...

Page 7: ...en turn the focus knob counter clockwise until sample comes into focus 2 Ensure you are focusing on correct sample plane and not another surface for example back side of a microscope slide If uncertain place a similar but easy to focus sample on stage focus and then switch to new sample Clicking on magnifier glass icon on top bar of Live Image Window zooms in on image so it fits the monitor screen...

Page 8: ...select Time Lapse Settings to open its dialog box 4 For brightfield images check White in the Activate column be sure FL is not checked Check Auto Gain ignore Gain and Exposure Do not click on Load Present Values 5 For fluorescent images not applicable to the LS400 check FL in the Activate column To set LED Gain and Exposure values use desired levels determined in Section II E above or determine n...

Page 9: ...ove J Recording live videos 1 First download and install the AviSynth and MeGUI programs as described in Section II H 1 above 2 Click red circle icon in top tool bar of Live Image Window to open Video Configuration dialog box 3 Choose Filename Root File Format TIFF recommended Destination Folder and if time stamp is desired Click OK and images will start to record 4 Click red circle icon again to ...

Page 10: ...4 9 3 Scale bar bug fix The scale was incorrect 14 7 9 Video capture at full frame rate using freeware the recommended freeware is used after the images are captured the same freeware could be used to convert time lapse images to video too optional scale bar on image optional date time stamp on image improved help finer LED brightness granularity robust behavior for plugging in and out of the USB ...

Page 11: ...iger Run Court Suite 112 Carlsbad CA 92010 760 298 2355 www etaluma com Driver Package Bitwise Systems QuickUSB has remained on your computer We recommend leaving the drivers installed if you may be going back and forth between different Lumascope models ...

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