Lumascope 500/400 Startup Guide
6
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lighting can cause uneven brightness including a striped pattern; if observed, move the LS500 or
partially shade the light to reduce unevenness across the sample.
For the best brightfield, use of the a) previously included white LED on flexible cable, b) included (or
purchased separately) Brightfield Illuminator LS500/400, or c) light source of the (optional) Phase
Contrast Accessory LS500/400 is recommended (see Section I.C. above). Using one of the 3 light
sources also allows control through Lumaview. Note: Although the LS500 camera is full color, a green
filter is installed for green fluorescence and thus brightfield images will be in black and white
(grayscale) or the same grayscale pseudo colored with green. The LS400 does not have the green filter
installed and thus the images will be full color brightfield.
3.
To connect the white LED on flexible cable, plug the USB end into the USB port on the back on the
LS500 (see Fig. 1). To connect the Brightfield Illuminator LS500/400, first remove the LS500 back panel
using the supplied 2 mm hex wrench and replace it with the new back panel attached to the Brightfield
Illuminator. Adjust the Brightfield Illuminator height by loosening the 2 screws on the back bracket
using the 2 mm hex wrench. If using the light source of the Phase Contrast Accessory LS500/400,
remove the slider (or use a slider position with no mask) and adjust the illumination brightness by
moving the horizontal lever on the Condenser front and/or the circular knob on the phase power cable.
4.
For viewing in a dark environment such as an incubator, a light source such as one of the 3 listed in
Section D.2 above is needed. Connecting one of these 3 light sources to the LS500 allows it to be
controlled by Lumaview 500/400. To mount the light source above the Lumascope deck, see Section
D.3 above.
5.
To illuminate the sample when using ambient light, move cursor over Live Image Window, right click,
and select Exposure Settings to open its dialog box. Check Auto Gain to most easily acquire the best
grayscale (ignore Gain % and Exposure % settings).
If using the white LED on flexible cable or the Brightfield Illuminator LS500/400, right click over Live
Image Window and select Illumination Settings to open its dialog box. Flip switch labeled B on focus
knob side of LS500 to the ON position. (The LS400 does not have the 2 switches on the side.) Right click
over Live Image Window again and select Exposure Settings to open its dialog box. In Illumination box,
check White LED. In Exposure box, check Auto Gain to most easily acquire the best grayscale (ignore
Gain % and Exposure % settings).
If using the light source of the Phase Contrast Accessory LS500/400, flip the switch on top of the
condenser to REMOTE to control the light through Lumaview 500/400. Follow the same steps as listed
in the previous paragraph for the white LED on flexible cable or Brightfield Illuminator LS500/400 to
illuminate the sample.
When using the light source of the Phase Contrast Accessory LS500/400, if the switch on top of the
condenser is in the ON position, the Phase Contrast Accessory will not take instructions from Lumaview
500/400 and there is no need for the phase signal cable (see the Phase Contrast Accessory User Guide
at
http://etaluma.com/products/downloads/
for more information). To turn on the light, ensure that
the phase power cable switch is in the ON position. The light can then be controlled by adjusting the
horizontal lever on the condenser front or turning the knob on the phase power cable.
