Lumascope 500/400 Startup Guide
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E. Illuminating the sample for fluorescence (not applicable to the LS400)
CAUTION
: When any LEDs are turned on, do NOT look directly at the light. Be sure to turn off all LEDs at
the end of a viewing session so any sample remaining on the deck is not photo-bleached.
1.
Choosing between green pseudo coloring and gray scale is a matter of choice. For green pseudo
coloring, right click over Live Image Window, select Colors and choose Green Only. For grayscale, right
click over Live Image Window, select Colors, and choose Green Only, Show as Gray.
2.
With cursor over Live Image Window, right click and select Illumination Settings to open its dialog box.
Right click again and select Exposure Settings to open its dialog box. Settings in these two dialog boxes
are typically adjusted together. Flip switch labeled F on focus knob side of Lumascope to the ON
position.
3.
In Illumination box, check FL LED for green fluorescence. Start with Gain as low as possible and
Exposure at maximum. Adjust Illumination to desired brightness. For dim samples, if Illumination is at
maximum level (> 50%), increasing the Gain can be used to increase the signal (but Gain will also
increase background as well).
F. Focusing
1.
Unless approximate sample focus level is known, it is best to initially raise the objective to maximum
height (without hitting sample) and then turn the focus knob counter-clockwise until sample comes
into focus.
2.
Ensure you are focusing on correct sample plane and not another surface, for example, back side of a
microscope slide. If uncertain, place a similar but easy to focus sample on stage, focus, and then switch
to new sample. Clicking on magnifier glass icon on top bar of Live Image Window zooms in on image so
it fits the monitor screen horizontally and should help you to focus. If necessary, increase or decrease
LED brightness and/or Gain.
G. Capturing images
1.
With cursor over Live Image Window, right click and select Image Parameters to open its dialog box.
Click on File Type dropdown menu and select format type desired. The TIFF format is recommended
due to several features as explained in Help/Image Types. Select or enter the Filename Root and Saving
Destination (e.g., a new folder set up on the desktop). Choose Image Rotation if desired.
Checking Auto Save Demand Capture will automatically save the images to your Destination Folder with
your Filename Root and a number based on time (incorporates day of the year down to milliseconds).
If not checked, a Save As dialog box will open every time an image is taken that allows naming and
choosing the destination for that image. Click Save to save your image settings.
2.
When desired field has been illuminated and focused in Live Image Window, hover the cursor over the
camera icon on top tool bar until it is outlined in blue. Left click the camera icon to capture the image.
3.
For quick review of your last image, click the folder icon on the top tool bar to launch Windows
Explorer, which will open the most recent Destination Folder.
