Lumascope 500/400 Startup Guide
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II. Software: Getting Started with Lumaview 500/400
The following information give instructions for getting started with Lumaview 500/400 and is intended as a
quickstart guide only. Not all options are given and not all features may be explained in any particular
section. The complete User Guide for Lumaview 500/400 is contained in the Help Section after the
program is opened and is accessible by clicking on F1 or the Help dropdown menu/Contents.
Becoming
familiar with the complete User Guide is a must in order to use the LS500 properly and take advantage of
the many features of this program.
A. Starting point conditions
1.
Lumaview 500/400 has been downloaded to the computer.
2.
The LS500 (Lumascope 500) or LS400 (Lumascope 400) is connected to the computer via the UBS
power/communication cable.
3.
Lumaview 500/400 is open and the Windows described in Section I.F.2 are visible.
4.
Sample to be imaged is present on the LS500 stage (and fluorescence shroud is over sample if imaging
involves fluorescence).
B. Select LS500 or LS400
1.
Click on the File dropdown menu in the upper navigation bar and select Set Microscope Version.
2.
Click on the appropriate Lumaview version.
C. Note about cursor
1.
In older Lumaview versions, the cursor may change to a camera when passed over the Live Image
Window.
2.
If you have this version,
you should download and install the latest version of Lumaview 500/400
(see
Section I.E above).
3.
It you must use the older version, when you left click your mouse or trackpad, either a Save As dialog
box will open or you will hear a clicking sound indicating that the camera has auto-saved an image.
4.
Read Section II.G on capturing images below. You can either uncheck the AutoSave function and cancel
the Save As dialog box whenever you left click, or leave the AutoSave function checked and be careful
to left click only when you want to record an image.
D. Illuminating the sample for brightfield
CAUTION
: When any LEDs are turned on, do NOT look directly at the light. Be sure to turn off all LEDs at
the end of a viewing session so that any sample remaining on the stage is not photo-bleached.
1.
LS500: For best contrast, it is recommended to set the colors to grayscale. Right click over Live Image
Window, select Colors, and choose Green Only, Show as Gray.
LS400: Right click over Live Image Window, select Colors, and choose RGB. Right click again, select
Colors and choose R/G/B Gain Adjustment. The Red/Green/Blue Gain dialog box will open and the
default settings will be present. Place a white sheet of paper over the deck hole (with or without
objective) and adjust the color gain settings so that the Live Image Window shows a gray color.
Typically reducing the Green gain to 64 will be an acceptable adjustment.
2.
For viewing in a typically lit area, ambient light is generally adequate to see brightfield. The light should
be as uniform as possible to prevent shadows or other dark or light areas. Overhead fluorescent
