Esco Cytoculture LS2-4A1, User Manual

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2. Turn on the fan by pressing the FAN button. Input the Fan PIN if asked (if PIN ≠ 0000). This will start
the warm up procedure (default: 3 minutes). All buttons are disabled during warm up period.
3. The BSC is ready for work.
3.2.2 Turning off the BSC
1. Turn off the fan by pressing the FAN button. Input the Fan PIN if asked (if PIN ≠ 0000). This will start
the post purge procedure (default: 0 minute). All buttons are disabled during post purge period.
2. Lower the sash to the fully closed position (the display will show UV MODE). The sash can be lowered
immediately after turning off the fan as it will not interrupt the post purge procedure.
3. Turn on the UV lamp (when present) to decontaminate the work area by pres sing the UV button.
Leave the UV lamp on to make sure the decontamination is done effectively . The UV lamp can
only be turned on after the post purge procedure is finished.
3.3 Working in the BSC

Surface-decontaminate the work area (work surface, back and side wall, UV lamp, electrical outlet,
service fixture and the inner surface of the sash window) before and after using the BSC.

Allow the BSC to purge any contaminant by allowing the blower to operate at least 3 minutes before
and after using the BSC.

Surface-decontaminate any item (including your arms) or apparatus with appropriate disinfectant
before entering or exiting the work area.

Place the waste container (containment bag, pipette discard pans, etc.) inside the BSC work area.

Place all items and apparatus in the safe working area.

Minimize room activity (personnel movements, closing and opening of doors, etc.) since these
external airflow disturbances may adversely affect the BSC’s internal airflow, thereby possibly
impairing the containment capabilities of the BSC.

Ensure that the sash is at normal operating height (READY state) before starting any experiment.

Ensure the front and back air grilles are not obstructed by your arms or any other objects.

Work as far inside the BSC as possible - at least 150 mm (6 inches) behind the front air intake grille.

While working in the BSC, move your hands slowly and in a controlled manner. Rapid movements may
disrupt the air barrier, allowing contaminants to escape or enter the BSC.

Place aerosol-generating instruments as far inside the BSC as possible and at least 150 mm (6 inches)
from clean items/materials.

Place air turbulence generating equipment such as centrifuges, blenders or sonicators towards the
back of the BSC. Stop other work while any of this equipment is in operation.

As far as possible, it is recommended that the BSC be operated continuously in order to achieve
optimal containment and cleanliness. Airflow studies have shown that once the fan has been
switched off, air from the BSC may escape due to the thermal currents from inside the BSC.
3.4 Working Ergonomics
On most occasions, you would most likely be operating the BSC in sitting rather than standing posture. There
are some obvious advantages of the sitting posture:

The physiological energy cost and fatigue involved in sitting are relatively less

Sitting posture provides the body with a stable support
SAFE WORKING AREA FOR MULTI
TRAY BSC