12
on for over 60 minutes or even overnight is not recommended because it shortens the lifespan of the
lamp. The UV lamps used in Esco BSC have a lifespan of 2,000 hours.
The UV lamp should be cleaned of any dust and dirt weekly and changed annually to ensure its
effectiveness.
The use of UV lamp in BSC has been explicitly discouraged in all major international standards and
recommendations.
3.6 Decontamination and Disinfecting Agents
For stainless steel surfaces, all common disinfecting agents except chlorine-based ones are suitable.
For powder coated surfaces, all common disinfecting agents are suitable. However, the BSC has been
specifically evaluated for use with the following:
o
1N hydrochloric acid
o
1N sodium hydroxide
o
1% quaternary ammonium compound
o
5% formaldehyde
o
5,000 ppm hypochlorite
o
2% iodophor
o
5% phenol
o
70% ethyl alcohol
Depending on the contaminant involved at the time of operating the BSC, there are various other
types of disinfecting agents that may be used. The following table outlines the effectiveness of various
disinfecting agents against the different types of contaminants.
Decontaminant
Glutaral-
dehyde
Peroxide/
Paracetic
acid/
Acetic acid
Chlorine
Dioxide
Chlorine
Iodophor
Alcohol
Phenolic
Quaternary
Ammonium
Compounds
Classification
Sterilant
Sterilant
Sterilant
High Level
Inter-
mediate
Inter-
mediate
Inter-
mediate
Low Level
Parameters for use:
Concentration
2%
1%
0.01-0.1%
0.01-5%
0.5-2.5%
70-85%
0.2-3%
0.1-2%
Contact time (min.)
10-600
10-720
10-600
10-30
10-30
10-30
10-30
10-30
Stability > 1 week
(1)
+
+
+
+
+
+
Agents:
Bacterial Endospores
+
+
+
+/-
Naked Viruses
+
+
+
+
+/-
+/-
+/-
Mycobacterium
+
+
+
+
+
+
+
Vegetative Bacteria
+
+
+
+
+
+
+
+
Enveloped Viruses
+
+
+
+
+
+
+
+
Characteristics:
Inactivated by
Organics
+
+
+
+
+/-
+
Residual
+
+
+
+/-
+
+
Corrosive
+
+
+
+
Flammable
+
Skin Irritant
+
+
+
+
+
+
Eye Irritant
+
+
+
+
+
+
+
Respiratory Irritant
+
+
+
+
+
+
+/-
Toxic
+
+
+
+
+
+
+
+
Use in BSCs:
Routine Surface
Decon
+/-
+
+
+
Biohazardous Spill
+/-
+
+/-
+
+
+/-
(1) protected from light and air
+ = effective, +/- = results may vary, blank = not effective
Summary of Contents for Cytoculture LS2-4A1
Page 7: ...Cytotoxic Lead Shielded Class II Biological Safety Cabinets v...
Page 9: ...Cytotoxic Lead Shielded Class II Biological Safety Cabinets vii...
Page 17: ...8...
Page 23: ...14...
Page 27: ...18...
Page 28: ...APPENDIX...
Page 29: ......
Page 31: ......
