Instruction Manual – 13
Nominal
volume
(µL)
Preset
volume
(μL)
Permissible
values (μL)
Volume Change ΔV (μL) for a
Turn of a Calibration Key by
1 Turn
1 Increment
2
0.2
0.176 - 0.224
0.06
0.0025
10
1
0.975 - 1.025
0.33
0.0137
20
2
1.94 - 2.06
0.63
0.0262
200
20
19.76 - 20.24
6.30
0.262
1,000
100
98.4 - 101.6
25.00
1.04
• Perform 10 aspirations, and calculate the average value
in [mg].
• Calculate the volume in [μL] by multiplying the value
in [mg] by the conversion factor Z [μL/mg].
If the average aspirated volume exceeds the permissible
values of the range, the pipette should be recalibrated.
Pipette Recalibration
K
1. Remove the pipetting pushbutton.
CAUTION:
The pipetting pushbutton consists of 2 parts:
the knob and the pushbutton. After removal of the
pushbutton, both parts are separated.
2. Holding the volume setting knob to prevent rotation,
insert the calibration key into the calibration screw.
3. Turn the key clockwise to reduce the aspirated volume,
or counter-clockwise to increase the aspirated volume.
Use the values given in the above table to precisely
adjust the volume.
K
1.
K KK
2.
3.
4.
